Colonization pattern of C. jejuni isolates of human and avian origin and differences in the induction of immune responses in chicken

Pielsticker, Colin; Glünder, G; Aung, Ye Htut; Rautenschlein, Silke

doi:10.1016/j.vetimm.2015.11.005

Cited by 18 publications

(36 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other side host factors, feed or other so far undefined factors may contribute to the evasion of C. jejuni from the gut. C. jejuni strain Lior6 was not detected in the liver of BT and LT birds in previous studies [26]. Interestingly, in this study C. jejuni invaded the liver of LT-bf birds which also showed the highest level of CFU of C. jejuni in the caecum.…”

Section: Discussioncontrasting

confidence: 56%

“…The C. jejuni strain of serogroup Lior6 had been isolated from a chicken at the Clinic of Poultry, University of Veterinary Medicine Hannover, Germany and was stored in skim milk at −70 °C [26]. …”

Section: Methodsmentioning

confidence: 99%

“…The role of T cells in the control of C. jejuni in mice and human beings was demonstrated, but little is known about T cell responses in chickens [15, 25, 26]. It has been suggested that C. jejuni infections in avian species are associated with Th1 polarization of the immune response [27, 28].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken

Han¹,

Willer²,

Pielsticker³

et al. 2016

Gut Pathog

Self Cite

View full text Add to dashboard Cite

BackgroundChickens are regarded as the main reservoir for human campylobacteriosis. Little is known about the interaction between Campylobacter jejuni (C. jejuni) and chickens. This interaction may be influenced by the stage of maturation of the immune system, developing gut microbiota composition and other factors including breed and diet. Our aim was to investigate the impact of breed, and diet on C. jejuni colonization and host immune responses in chickens. Birds were inoculated with 104 colony forming units (CFU) of C. jejuni or diluent at one (Exp. 1) or 22 (Exp. 2) days post hatch. We compared local immune cell subpopulations, cytokine expression levels, and gut microbiota composition between broiler-type (BT) and layer-type (LT) birds fed with either commercial broiler feed (bf) or layer feed (lf).ResultsLower colonization rates were observed in the older age group independent of breed and diet. Independent of breed, birds fed with bf showed higher CFU of C. jejuni compared to lf-fed groups. Campylobacter jejuni-inoculation had a significant effect on lymphocyte numbers and cytokine expression levels in BT birds independent of feeding strategy (p < 0.05). These effects were not detected in LT birds, only LT birds fed with bf showed a significant increase in IL-8-expression at 7 days post C. jejuni inoculation compared to LT-control birds (p < 0.05). Diet influenced gut microbiota composition in a comparable manner between BT and LT birds, but changes in microbiota composition associated with C. jejuni inoculation varied between breeds.ConclusionsDiet and breed influenced C. jejuni colonization, immune responses and microbiota composition to a different extent comparing between LT and BT birds. The mechanisms behind these differences have to be elucidated further. Our results suggest that selection for more resistant breeds in combination with adapted feeding strategies may help to reduce Campylobacter colonization levels in commercial poultry in the future.

show abstract

Section: Discussioncontrasting

confidence: 56%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken

Han¹,

Willer²,

Pielsticker³

et al. 2016

Gut Pathog

Self Cite

View full text Add to dashboard Cite

show abstract

“…The immune system plays an important role in the mucosal defense against bacterial infections. T-cell-mediated immunity was demonstrated in the control of C. jejuni colonization in mice and humans, but little is known about the role of T cells in the control of C. jejuni infection in chickens ( 10 – 12 ). It has been suggested that C. jejuni infections in avian species are associated with Th1 polarization of the immune response ( 3 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Gut Microbiota Composition on Campylobacter jejuni Colonization in Chickens

Han¹,

Willer²,

Li³

et al. 2017

Infect Immun

Self Cite

View full text Add to dashboard Cite

The Campylobacter jejuni-host interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites, or modification of the immune response. To understand this interaction, C. jejuni colonization and local immune responses were compared in chickens with different gut microbiota compositions. Birds were treated with an antibiotic cocktail (AT) (experiments 1 and 2) or raised under germfree (GF) conditions (experiment 3). At 18 days posthatch (dph), they were orally inoculated either with 104 CFU of C. jejuni or with diluent. Cecal as well as systemic C. jejuni colonization, T- and B-cell numbers in the gut, and gut-associated tissue were compared between the different groups. Significantly higher numbers of CFU of C. jejuni were detected in the cecal contents of AT and GF birds, with higher colonization rates in spleen, liver, and ileum, than in birds with a conventional gut microbiota (P < 0.05). Significant upregulation of T and B lymphocyte numbers was detected in cecum, cecal tonsils, and bursa of Fabricius of AT or GF birds after C. jejuni inoculation compared to the respective controls (P < 0.05). This difference was less clear in birds with a conventional gut microbiota. Histopathological gut lesions were observed only in C. jejuni-inoculated AT and GF birds but not in microbiota-colonized C. jejuni-inoculated hatchmates. These results demonstrate that the gut microbiota may contribute to the control of C. jejuni colonization and prevent lesion development. Further studies are needed to identify key players of the gut microbiota and the mechanisms behind their protective role.

show abstract

“…Thus, due to certain surface structures (pathogen-associated molecular patterns) of corresponding receptors (pattern recognition receptors) of the intestinal immune system of the broiler, C. jejuni is recognized as potentially pathogenic. Furthermore, it induces an increase in proinflammatory cytokines such as IL-1 and IL-6, which function as messenger substances in the organism ( 59 – 62 ). In addition to many other functions, these messenger substances also activate mucin release ( 56 – 58 ).…”

Section: Discussionmentioning

confidence: 99%

Feed Choice Led to Higher Protein Intake in Broiler Chickens Experimentally Infected With Campylobacter jejuni

et al. 2018

View full text Add to dashboard Cite

In 2016, Campylobacter was the most commonly reported gastrointestinal bacterial pathogen in humans in the European Union with 246,307 reported cases. Of these cases, 83.6% were Campylobacter jejuni. The objective of the present study was to investigate to what extent an infection with C. jejuni alters the feed intake behavior of broiler chicks in terms of protein intake. This was done to see if, conversely, measures of control could be derived. In total, 300 commercial broilers of the Ross 308 line were allocated to four different groups, including five replications of 15 chickens each. In two groups, a conventional diet [216 g CP/kg dry matter (DM)] and in the two choice diet groups, diets with different levels of crude protein (286 and 109 g CP/kg DM, respectively) were fed between day 14 and day 42. An intake of both choice diets at a ratio of 3:2 resulted in a composition of consumed feed identical to that of the control concerning composition, energy and nutrient content. One group of each feeding concept was infected artificially with C. jejuni at day 21 by applying an oral C. jejuni-suspension containing 5.26 ± 0.08 log10 colony forming units of C. jejuni to three out of 15 chickens. No significant differences concerning C. jejuni prevalence and excretion could be seen. Broilers infected with C. jejuni chose a higher amount of the high protein choice diet in comparison to C. jejuni negative broilers. This resulted in a significantly (p < 0.0001) higher content of crude protein in the consumed diet (198 ± 3.09 g CP/kg DM and 208 ± 8.57 g CP/kg DM, respectively). Due to C. jejuni infection, a significant increase in crude mucin in excreta at day 42 was seen in experimentally infected groups (62.6 ± 4.62 g/kg DM vs. 59.6 ± 6.21 g/kg DM, respectively; p = 0.0396). There were significantly deeper crypts in infected birds (256 ± 71.6 vs. 234 ± 61.3 μm). In summary, C. jejuni infections significantly alter the feed intake behavior of broiler chickens regarding higher protein intake. Therefore, targeted manipulation of protein supply could be tested for limiting the spread of infection.

show abstract

Colonization pattern of C. jejuni isolates of human and avian origin and differences in the induction of immune responses in chicken

Cited by 18 publications

References 51 publications

Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken

Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chicken

Influence of the Gut Microbiota Composition on Campylobacter jejuni Colonization in Chickens

Feed Choice Led to Higher Protein Intake in Broiler Chickens Experimentally Infected With Campylobacter jejuni

Contact Info

Product

Resources

About