Colony Characteristics, Nucleoside Analog Profiles, and Genetic Variations of Medicinal Fungus Polycephalomyces nipponicus (Ascomycetes) Isolates from Northeast Thailand

Sangdee, Aphidech; Sangdee, Kusavadee; Seephonkai, Prapairat; Jaihan, Piyanoot; Kanyaphum, Tewika

doi:10.1615/intjmedmushrooms.v19.i5.60

Cited by 9 publications

(3 citation statements)

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“…Among the fungal isolates, the Cod-KK1643 isolate had the most potent antifungal activity, as evidenced by large inhibition zones, relative to the antibiotic amphotericin B, and it had the lowest of MIC value. This result is in agreement with the finding that antifungal activity depends on the fungal strains [20]. Therefore, Cod-KK1643 was chosen for further studies on elucidation of antifungal activity with time kill assay and SEM.…”

Section: Discussionsupporting

confidence: 88%

Effects of mycelial extract and crude protein of the medicinal mushroom, Ophiocordyceps sobolifera, on the pathogenic fungus, Candida albicans

Sangdee¹,

Sangdee²,

Buranrat³

et al. 2019

Trop. J. Pharm Res

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Purpose: To investigate the antifungal effect of the mycelial extracts and crude proteins of the medicinal mushroom, Ophiocordyceps sobolifera on Candida albicans. Methods: The antifungal activities of the mycelial extracts and crude proteins of seven strains of the entomopathogenic fungus, Ophiocordyceps sobolifera, were screened against Candida albicans strain NCYC854 using an agar well diffusion method. Minimum inhibitory concentrations (MIC) and minimum fungicidal concentration (MFC) were determined using broth microdilution method. The kinetics of fungal death was elucidated via time-kill assays, and while ultrastructural alteration changes to fungal cells were investigated by scanning electron microscopy (SEM). Results: The antifungal activities of the mycelial extracts were superior to those of the crude proteins. Among the isolates, Cod-KK1643 exhibited the highest activity with the lowest MIC against the test strains. Therefore, it was chosen for further investigations. The isolate Cod-KK1643 exhibited concentration-and time-dependent fungistatic activity in the time kill assay. However, these activities were absent in the crude protein of the isolate. Moreover, Cod-KK1643 mycelial extract induced morphological alterations in fungal cells, such as decreased cell size, and crushed or cracked appearance. Slight alterations in cell morphology (decreased cell size or crushed appearance) were observed in the crude protein treatment. Conclusion: The mycelial extracts of the fungus, O. sobolifera (especially isolate Cod-KK1643) exert potent antifungal activity against human pathogenic fungus, C. albicans.

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Section: Discussionsupporting

confidence: 88%

Effects of mycelial extract and crude protein of the medicinal mushroom, Ophiocordyceps sobolifera, on the pathogenic fungus, Candida albicans

Sangdee¹,

Sangdee²,

Buranrat³

et al. 2019

Trop. J. Pharm Res

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“…Nhưng sự biến đổi đặc điểm hình thái chỉ có thể quan sát ở một số trường hợp (A. Sangdee, Sangdee, Seephonkai, Jaihan, & Kanyaphum, 2017). Kết quả nghiên cứu phát sinh loài phân tử của Sung, Sung, Jones, và Spatafora (2007) Mặt khác, nhiều loài nấm kí sinh trên ấu trùng ve sầu có sự nhầm lẫn về hình dạng dẫn đến việc đặt tên không đúng khi quan sát hình thái đại thể của chúng (Jaihan & ctg., 2021).…”

Section: Giới Thiệuunclassified

Phát hiện loài nấm polycephalomyces nipponicus ký sinh trên ấu trùng ve sầu tại thị trấn Ea Knốp, Tỉnh Đăk Lăk, Việt Nam

Hạnh¹,

Lao²,

Le³

et al. 2022

HCMCOUJS - KỸ THUẬT VÀ CÔNG NGHỆ

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Mẫu nấm H022, ký sinh trên ấu trùng ve sầu, được phát hiện ở khu vực thị trấn Ea Knốp - Tỉnh Đăk Lăk. Mẫu nấm được thu nhận, đưa về phòng thí nghiệm để giải phẫu hình thái nhằm xác định tên loài. Đồng thời, DNA bộ gene được thu nhận bằng phương pháp phenol/chloroform để tiến hành khuếch đại các gene mục tiêu nrSSU, ITS, nrLSU, Rpb1, Tef1 bằng kỹ thuật PCR. Sản phẩm khuếch đại được giải trình tự và tiến hành phân tích phát sinh loài phân tử để hỗ trợ việc định danh. Kết quả phân tích hình thái khẳng định rằng mẫu H022 chính là loài Polycephalomyces nipponicus (Kobayasi) (Kepler & ctg., 2013). Kết quả phân tích phát sinh loài phân tử cho thấy mẫu H022 hình thành nhóm đơn huyết thống với trình tự loài Polycephalomyces nipponicus với giá trị bootstrap lần lượt là 100/100/100 (Neighbor-Joining (NJ), Maximum Parsimony (MP), and Maximum Likelihood (ML)). Kết quả giải phẫu hình thái và phát sinh loài phân tử đều khẳng định mẫu nấm H022 là loài Polycephalomyces nipponicus (Kobayasi) (Kepler & ctg., 2013). Ngoài ra, đây là ghi nhận đầu tiên loài Polycephalomyces nipponicus tại Đăk Lăk.

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“…exhibit significant potential for producing a diverse range of secondary metabolites. For instance, Pleurocordyceps nipponicus and P. phaothaiensis contain natural antioxidant, antibacterial, antitumorigenic, anti-inflammatory, and antimicrobial compounds (Sangdee et al, 2017 ; Somsila et al, 2018 ; Sonyot et al, 2020 ). Gokhale et al ( 2020 ) reported that the secondary metabolites of P. sinensis have antibacterial potential.…”

Section: Introductionmentioning

confidence: 99%

Four novel Pleurocordyceps (Polycephalomycetaceae) species from China

Xiao,

Yang,

Jayawardena

et al. 2024

Front. Microbiol.

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Entomopathogenic fungi comprise an ecologically important group of specialized pathogens infecting other fungi, invertebrates, and plants. These fungi are species-rich with high diversity and broad distribution worldwide. The majority of entomopathogenic fungi belong to clavicipitoids, which consist of the hypocrealean families, Clavicipitaceae, Cordycipitaceae, Ophiocordycipitaceae, and Polycephalomycetaceae. The latter is a newly established entomopathogenic family that recently separated from the family Ophiocordycipitaceae to accommodate the genera, Perennicordyceps, Pleurocordyceps, and Polycephalomyces. In recent years, Polycephalomycetaceae has been enriched with parasitic and hyperparasitic fungi. With 16 species spread across China, Ecuador, Japan, and Thailand, Pleurocordyceps is the most speciose genus in the family. In this study, we expand the number of taxa in the genus by introducing four new Pleurocordyceps species from China, namely, P. clavisynnema, P. multisynnema, P. neoagarica, and P. sanduensis. We provide detailed descriptions and illustrations and infer genus-level phylogenies based on a combined 6-loci gene sequence dataset comprising the internal transcribed spacer gene region (ITS), small subunit ribosomal RNA gene region (SSU), large subunit rRNA gene region (LSU), translation elongation factor 1-alpha gene region (TEF-1α), RNA polymerase II largest subunit gene region (RPB1), and RNA polymerase II second largest subunit (RPB2). This study contributes to knowledge with regard to the diversity of Pleurocordyceps specifically and entomopathogenic Hypocreales more broadly.

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Colony Characteristics, Nucleoside Analog Profiles, and Genetic Variations of Medicinal Fungus Polycephalomyces nipponicus (Ascomycetes) Isolates from Northeast Thailand

Cited by 9 publications

References 0 publications

Effects of mycelial extract and crude protein of the medicinal mushroom, <i>Ophiocordyceps sobolifera</i>, on the pathogenic fungus, <i>Candida albicans</i>

Effects of mycelial extract and crude protein of the medicinal mushroom, <i>Ophiocordyceps sobolifera</i>, on the pathogenic fungus, <i>Candida albicans</i>

Phát hiện loài nấm polycephalomyces nipponicus ký sinh trên ấu trùng ve sầu tại thị trấn Ea Knốp, Tỉnh Đăk Lăk, Việt Nam

Four novel Pleurocordyceps (Polycephalomycetaceae) species from China

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