Colony formation by normal and malignant human B-lymphocytes

Izaguirre, Carlos A.; Minden, Mark D.; Howatson, A.F.; McCulloch, E. A.

doi:10.1038/bjc.1980.255

Cited by 67 publications

(12 citation statements)

References 13 publications

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“…When 4 x 105 cells of the 10 marrow preparations of 9 patients were plated, a sufficiently high plating efficiency (0-15-1.0%) was obtained to allow a reliable comparison between test and control assays. This plating efficiency is similar to that reported by Izaguirre et al (1980) but much higher than that reported by Hamburger and Salmon (1977).…”

Section: Discussionsupporting

confidence: 91%

“…Using the modified method developed by Izaguirre et al (1980) human myeloma stem cells could be detected by their ability to form colonies. When 4 x 105 cells of the 10 marrow preparations of 9 patients were plated, a sufficiently high plating efficiency (0-15-1.0%) was obtained to allow a reliable comparison between test and control assays.…”

Section: Discussionmentioning

confidence: 99%

“…The mononuclear cell fraction from our 9 patients contained 85-95% myeloma cells. These Culture method.-Culture methods for myeloma colony formation described by Izaguirre et al (1980) were used, with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

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Proliferative state and radiosensitivity of human myeloma stem cells

Shimizu¹,

Motoji²,

Oshimi³

et al. 1982

Br J Cancer

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Summary.-Human myeloma stem cells were detected by their capacity to form colonies in culture. Cells separated from aspirated marrow were cultured for 10 days in semi-solid methylcellulose with medium conditioned by T lymphocytes stimulated by phytohaemagglutinin (PHA-TCM). The colonies formed consisted mostly of lymphoplasmacytoid cells or plasma cells, and the immunoglobulins in the patients' myeloma cells were demonstrated also in the cytoplasm of the colony cells. The number of colonies were proportional to the number of cells plated and to the concentration of PHA-TCM. When the proportion of proliferating colonyforming units of multiple myeloma (CFU-MM) was studied using the (3H)-dT-suicide technique, the high-specific-activity (3H)-dT killed 21-45% of the CFU-MM in 7 myeloma patients. According to a single dose of 60Co-y-irradiation, the mean doses for impairment of regeneration (Do) were 1-00 and 1.63 Gy in 2 cases, the extrapolation numbers being 1.6 and 2.0.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Proliferative state and radiosensitivity of human myeloma stem cells

Shimizu¹,

Motoji²,

Oshimi³

et al. 1982

Br J Cancer

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“…The excess of OKT8/Leu-2a+ T cells over OKT4/Leu-3a+ T cells commonly observed in this series is also observed, though less commonly, in reactive lymph nodes, but does not alone imply a reaction by "cytotoxic" T cells against "neoplastic" B cells. Moreover, there is good evidence that the clonogenic components of NHL B cells are, like normal B cells, dependent upon T cell factors, and T cells, for growth (Izaguirre et al, 1980).…”

Section: Phenotypic Heterogeneity In Peripheral Tcellsmentioning

confidence: 99%

The cellular content of non Hodgkin lymphomas: A comprehensive analysis using monoclonal antibodies and other surface marker techniques

Habeshaw¹,

Bailey²,

Stansfeld³

et al. 1983

Br J Cancer

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Summary Five samples of tonsil, 10 reactive lymph nodes and 65 consecutive cases of non-Hodgkin lymphoma (NHL) were evaluated in suspension phenotyping with the monoclonal antibodies aLeu-I, aLeu-2a, aLeu-3a, OKT1, OKT3, OKT4, OKT6, OKT8, W6/32, 26/114, DA-2, 2DI, J5, AN51 and OKT9 together with conventional surface marking by rosetting (E, Fcy, Fcts, C3b, C3d) Previous reports on phenotyping in non Hodgkin's lymphoma (NHL) indicate marked heterogeneity within tumours of similar appearance and histological class. Nevertheless such studies have shown broad correlation between histological class and phenotype (Jaffe et al., 1974;Gajl-Peczalska et al., 1975; Stein, 1976; Stathopoulos et al., 1977; Brouet, et al., 1977;Jaffe et al., 1977;Lukes et al., 1978; Stein et al., 1979;Habeshaw et al., 1979;Janossy et al., 1980;Aisenberg, 1981) A significant effect of these studies has been to focus attention on the cellular origin, differentiation and possible pathogenic role of the cell populations within the lesions. This applies particularly to the T cell populations accompanying B lymphocytic neoplasia of follicular and immunoblastic types (Habeshaw et al., 1979;Janossy et al., 1980), which until recently were evaluable only on the basis of E rosette formation, or reactivity with heteroantisera of pan T cell or limited T cell subset specificity (Evans et al., 1978

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“…22,23 Clonogenic methods for B and T lymphocytes were also described. 24,25 Colony formation in culture depends of the presence of bioactive stimulators, colony-stimulating factors or CSF. The rapid development of methods for studying molecules were quickly applied to CSFs.…”

Section: Colonies In Culturementioning

confidence: 99%

Stem cells and diversity

McCulloch

2003

Leukemia

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Modern hematology began with two technology-based revolutions. First, assays were developed for cells earlier in lineages than those recognized morphologically. These opened for study important areas such as regulation and response to injury. Second, the rapid development of molecular biology disclosed complex mechanisms within cells and identified important genetic changes in malignancy. When immediate causative lesions were found, specific treatments directed at them were seen to be highly effective.Recently, several groups have provided evidence of the existence in adult tissues of stem cells that are capable of differentiation into such diverse organ systems as hematopoiesis, muscle, liver and brain. The implications of this stem cell plasticity are just beginning to be realized.The purpose of this paper is to review some of the concepts, advances and problems emerging from colony assays, molecular biology and stem cell plasticity. A hypothesis will be presented that links stem cell plasticity to the clinical entity known as metastases with unknown primary source. Colony assaysClonogenic assays, where a single cell gives rise to a recognizable colony, are selective, since they can measure minority populations; the assays are specific, quantitative, qualitative and provide information about the proliferative and differentiation capacities of the cells of origin. Each of these advantages of colony assays has limitations. The most severe limitation of the method is that analysis of cellular composition to determine progenitor properties shows only what the cell of origin did during clonal expansion, but not what it might do if conditions were improved or the time of observation prolonged.

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Colony formation by normal and malignant human B-lymphocytes

Cited by 67 publications

References 13 publications

Proliferative state and radiosensitivity of human myeloma stem cells

Proliferative state and radiosensitivity of human myeloma stem cells

The cellular content of non Hodgkin lymphomas: A comprehensive analysis using monoclonal antibodies and other surface marker techniques

Stem cells and diversity

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