Colorimetric and fluorometric studies on the borntraeger reaction for anthraquinone drugs

Context: Dillenia indica Linn. (Dilleniaceae) is traditionally used to treat skin inflammation. Objective: This study evaluated the healing effect of Dillenia indica fruit extracts on induced psoriasis-like wounds in Wistar rats. Materials and methods: Extracts were standardized to betulinic acid, including an aqueous ethanolic extract (AEE), ethyl acetate extract (EAE) and petroleum ether extract. Effects against lipid peroxidation were assessed in vitro. Wounds were created at rat tails (n = 12). Topical treatments were applied once daily for 7 days (1 mL of AEE or EAE at 5 or 50 mg/mL). Maximal dose was defined by the extract solubility. A 10-fold lower dose was also tested. Positive and negative controls were treated with clobetasol (0.5 mg/mL) or excipient. Half of each group was euthanized for histology. The remaining animals were observed for 20 days for wound measurements. Results: Yields of AEE and EAE were 4.3 and 0.7%, respectively. Betulinic acid concentrations in AEE and EAE were 4.6 and 107.6 mg/g. Extracts neutralized lipid peroxidation in vitro at 0.02 μg/mL, accelerating healing at 50 mg/mL. Complete healing in mice treated with AEE occurred 16 days after wound induction. This time was 14 and 12 days in mice treated with EAE and clobetasol. Compared to orthokeratosis, parakeratosis was reduced by AEE (25%), EAE (45%) and clobetasol (55%). EAE caused superior protection against biomolecules oxidation of skin compared to AEE. Discussion and conclusion: EAE exhibited activity closer to that of clobetasol. Betulinic acid may be an active constituent, which should be assessed in future studies.

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“…2009 ). Anthraquinones and anthracenes were evaluated using the Borntraeger reaction (Christensen & Abdel-Latif 1949 ).…”

Section: Methodsmentioning

confidence: 99%

Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats

Kviecinski

David

Fernandes

et al. 2016

Pharmaceutical Biology

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“…Extracts of each insect (0.1 g/ml) were subjected to preliminary phytochemical screening (acidic, alkaline and neutral aqueous systems were used when necessary) by standard methods [17] for detection of the following constituents: tannins [18], alkaloids, triterpenoids, carbohydrates, steroids [19], saponins, glucosides [20], flavonoids [21], and anthraquinones [22].…”

Section: Qualitative Phytochemical Analysismentioning

confidence: 99%

Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family

et al. 2020

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In the case of Tenebrionidae family insects, studies focus on larval stage, leaving a lack of information regarding other stages. Therefore, this study was performed in order to understand the differences between the nutritional composition and the bioactivity of two species of this family in their adult stage, fed with a specific diet. Adult beetles of both species were defatted, lyophilized and protein extracted with buffer. Proximal and phytochemical analysis of the extracts of each insect were performed, along with protein extract and hydrolysis analysis by Tris-Tricine and Tris Glycine SDS PAGE. This analysis showed that T. molitor contained more protein and fat than U. dermestoides but contained less crude fiber. The protein extraction was made with PBS, where 130 and 45 kDa bands showed predominant for U. dermestoides, and less protein was present for T. molitor. Antioxidant and antimicrobial activities of the enzymatic protein hydrolysates and protein crude extracts were determined. Presence of protein associated with the antioxidant activity were found in both insects. Nonetheless U. dermestoides had a higher antioxidant activity with the protein extract in contrast with the higher antioxidant activity shown by U. dermestoides once the extracts were digested. After proteolysis, protein extracts showed an increasing antioxidant activity, plus, the ability to inhibit microbial growth of Proteus, Shigella and Bacillus. Insect protein hydrolysates with protease open the possibility for the use of these beetles as new sources of encrypted peptides for microbiological control once characterized.

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“…A modification of the method of White and Rolf ( 278) for the determination of diodrast (diodone) has eliminated the need for special apparatus (26). Borntraeger's reaction has been utilized in the development of colorimetric and fluorometric methods of assay of Alexandria senna leaves (52) and certain anthraquinone drugs (51).…”

Section: Generalmentioning

confidence: 99%

Pharmaceuticals and Natural Drugs

Marsh¹,

Hilty²

1950

Anal. Chem.

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Colorimetric and fluorometric studies on the borntraeger reaction for anthraquinone drugs

Cited by 7 publications

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Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats

Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats

Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family

Pharmaceuticals and Natural Drugs

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