Colorimetric and label free detection of gelatinase positive bacteria and gelatinase activity based on aggregation and dissolution of gold nanoparticles

Mortezaei, Monireh; Dadmehr, Mehdi; Korouzhdehi, Behnaz; Hakimi, Mohammad; Ramshini, Hossein

doi:10.1016/j.mimet.2021.106349

Cited by 23 publications

(8 citation statements)

References 21 publications

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“…Thus, the integration of core–shell structures with graphene would enhance the performance of organic pollutant degradation and result in the increment of the photocatalytic properties. There are several reports on different biological applications of nanomaterials. − Among the different biological assays, cell cytotoxicity would be a crucial and determining factor for synthesized nanostructures, which was also discussed in the previous studies . In the present study, we constructed an effective and low-cost photocatalyst for RhB degradation.…”

Section: Introductionmentioning

confidence: 84%

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

et al. 2022

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Development of light-harvesting properties and inhibition of photogenerated charge carrier recombination are of paramount significance in the photocatalytic process. In the present work, we described the synthesis of core–shell heterostructures, which are composed of titanium oxide (TiO 2 ) and cerium oxide (CeO 2 ) deposited on a reduced graphene oxide (rGO) surface as a conductive substrate. Following the synthesis of ternary rGO-CeO 2 @TiO 2 and rGO-TiO 2 @CeO 2 nanostructures, their photocatalytic activity was investigated toward the degradation of rhodamine B dye as an organic pollutant under UV light irradiation. The obtained structures were characterized with high-resolution transmission electron microscopy, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Brunauer–Emmett–Teller, X-ray photoelectron spectroscopy surface analysis, and UV–Vis spectroscopy. Various parameters including pH, catalyst dosage, temperature, and contact time were studied for photocatalysis optimization. Heterostructures showed considerable advantages because of their high surface area and superior photocatalytic performance. In contrast, rGO-CeO 2 @TiO 2 showed the highest photocatalytic activity, which is attributed to the more effective electron–hole separation and quick suppression of charge recombination at core–shell phases. A biological assay of the prepared heterostructure was performed to determine the cytotoxicity against breast cancer cells (MCF-7) and demonstrated a very low survival rate at 7.65% of cells at the 17.5 mg mL –1 concentration of applied photocatalyst.

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Section: Introductionmentioning

confidence: 84%

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

et al. 2022

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“…Growth media supplement the protein substrates, which were then poured into Petri plates. Commonly used substrates include skim milk agar ( Masi et al, 2021 ; Shaikh et al, 2023 ), casein agar ( Yokota et al, 1988 ; Rathod and Pathak, 2014 ), bovine serum albumin (BSA) agar ( De Azeredo et al, 2001 ), gelatin agar ( Mortezaei et al, 2021 ), keratin agar ( Pereira et al, 2014 ; Nnolim et al, 2020 ), fibrin agar ( Prabhu et al, 2021 ; Anis Ahamed et al, 2022 ), and elastin agar ( Zins et al, 2001 ). When protease was produced in liquid media, the supernatant of fermentation broth (for extracellular proteases) or cell lysate (intracellular proteases) containing protease was collected.…”

Section: Detection Of Microbial Proteasesmentioning

confidence: 99%

Microbial proteases and their applications

Song,

Zhang,

Wang

et al. 2023

Front. Microbiol.

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Proteases (proteinases or peptidases) are a class of hydrolases that cleave peptide chains in proteins. Endopeptidases are a type of protease that hydrolyze the internal peptide bonds of proteins, forming shorter peptides; exopeptidases hydrolyze the terminal peptide bonds from the C-terminal or N-terminal, forming free amino acids. Microbial proteases are a popular instrument in many industrial applications. In this review, the classification, detection, identification, and sources of microbial proteases are systematically introduced, as well as their applications in food, detergents, waste treatment, and biotechnology processes in the industry fields. In addition, recent studies on techniques used to express heterologous microbial proteases are summarized to describe the process of studying proteases. Finally, future developmental trends for microbial proteases are discussed.

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“…Gelatin is in a cationic state when in an acidic environment; it is in an anionic state when in an alkaline environment. , Gelatinase, a bacterial enzyme that targets the hydrolysis of gelatin to generate amino acids, can be secreted. Amino acids can boost resistance and stop microorganisms from reentering an animal’s body . An anionic polymer with a variety of uses, xanthan gum is a type of extracellular microbial polysaccharide .…”

Section: Introductionmentioning

confidence: 99%

“…Amino acids can boost resistance and stop microorganisms from reentering an animal's body. 14 An anionic polymer with a variety of uses, xanthan gum is a type of extracellular microbial polysaccharide. 15 Xanthan gum is now regarded as the best biogum available due to its exceptional water retention and biocompatibility.…”

Section: ■ Introductionmentioning

confidence: 99%

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

Li,

Liu,

Dou

et al. 2024

Langmuir

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Tildipirosin has no significant inhibitory effect on intracellular bacteria because of its poor membrane permeability. To this end, tildipirosin-loaded xanthan gum− gelatin composite nanogels were innovatively prepared to improve the cellular uptake efficiency. The formation of the nanogels via interactions between the positively charged gelatin and the negatively charged xanthan gum was confirmed by powder X-ray diffraction and Fourier transform infrared. The results indicate that the optimal tildipirosin composite nanogels possessed a 3D network structure and were shaped like a uniformly dispersed ellipse, and the particle size, PDI, and ζ potential were 229.4 ± 1.5 nm, 0.26 ± 0.04, and −33.2 ± 2.2 mV, respectively. Interestingly, the nanogels exhibited gelatinase-responsive characteristics, robust cellular uptake via clathrin-mediated endocytosis, and excellent sustained release. With those pharmaceutical properties provided by xanthan gum−gelatin composite nanogels, the anti-Staphylococcus aureus activity of tildipirosin was remarkably amplified. Further, tildipirosin composite nanogels demonstrated good biocompatibility and low in vivo and in vitro toxicities. Therefore, we concluded that tildipirosin-loaded xanthan gum−gelatin composite nanogels might be employed as a potentially effective gelatinase-responsive drug delivery for intracellular bacterial infection.

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Colorimetric and label free detection of gelatinase positive bacteria and gelatinase activity based on aggregation and dissolution of gold nanoparticles

Cited by 23 publications

References 21 publications

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Microbial proteases and their applications

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

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Colorimetric and label free detection of gelatinase positive bacteria and gelatinase activity based on aggregation and dissolution of gold nanoparticles

Cited by 23 publications

References 21 publications

Fabrication of Graphene-Based TiO2@CeO2 and CeO2@TiO2 Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO2@CeO2 and CeO2@TiO2 Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Microbial proteases and their applications

Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum–Gelatin Composite Nanogels

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Product

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Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity