Colorimetric and ratiometric fluorescent detection of sulfite in water via cationic surfactant-promoted addition of sulfite to α,β-unsaturated ketone

Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

“…Hence, fluorescent probes for sulfite and/or bisulfite have been reported [15][16][17][18] and used for imaging of intracellular sulfite/bisulfite levels [19][20][21]. However, to the best of our knowledge, no probes for the determination of sulfite distribution in subcellular organelles are available.…”

An effective colorimetric and ratiometric fluorescent probe for bisulfite in aqueous solution

“…1B). The proposed sensing mechanism involves Michael addition between bisulfite and the a,b-unsaturated ketone [46,47]. The nucleophilic addition of HSO 3 À to the electrophilic C]C double bond shortens the p-conjugation of probe BICO (Scheme 1).…”

“…Three reported probes with large emission wavelengths (l em > 640 nm) use dual excited wavelengths and respond to bisulfite and sulfite [45,48,49]. Two bisulfite probes with a,b-unsaturated ketone group exhibit good properties in practical samples, but they lack living cell imaging [46,47]. Therefore, probes with high sensitivity for cell imaging are needed.…”

A fast‐responsive fluorescent probe for sulfite and its bioimaging

“…As an alternative, the fluorescence method has received much attention due its the advantages of convenience, high sensitivity, fast response and ease of live-cell imaging (14)(15)(16). Fluorescent probes for SO 3 2-have been mainly based on the addition to an aldehyde moiety or a nucleophilic addition (17)(18)(19)(20)(21)(22)(23)(24)(25), and levulinate cleavage mediated by SO 3 2- (26)(27)(28)(29)(30)(31)(32)(33)(34)(35). However, these probes suffer from low selectivity in the presence of biothiols such as cysteine, homocysteine, glutathione and a relatively long reaction time, which are not suitable for detecting or imaging SO 3 2-in living cells.…”