Colorimetric assay of phosphate using a multicopper laccase-like nanozyme

Tris-Copper Nanozyme as a Novel Laccase Mimic for the Detection and Degradation of Phenolic Compounds

“…To study the practical applications of the Tris-Cu nanozyme, the effects of Cl ions and ethanol content on the catalytic activity of the Tris-Cu nanozyme and natural laccase are investigated, respectively. As shown in Figure 4A, Cl ions can promote the oxidation of the substrate by the Tris-Cu nanozyme, which is consistent with the findings reported in the literature [26,[32][33][34]. The reason for this phenomenon is that Cl ions, as inhibitors of laccase, can bind to the type 2/3 (T2/T3) trinuclear copper site of laccase, resulting in the inactivation of laccase [35].…”

A new strategy for RRS-based determination of phosphate with a bifunctional Fe3O4 magnetic nanoparticle surface molecularly imprinted polydopamine probe

“…Electrochemical 32 and fluorescence 33 methods have the advantages of wide measuring range and high sensitivity, but the experimental operation or material preparation is more complicated. Colorimetric method, 34 continuous flow analysis method 35 and this experimental method have the advantages of simple and rapid, and the detection range of continuous analysis is too narrow. In terms of the measurement range, the colorimetric method and this study's method have certain limitations, but based on the magnetic enrichment ability of the experimental material, the concentration of phosphate detected by this experimental method can reach 0.1 μmol L −1 under the condition that the measurement error is tolerable.…”

Nanozymes: powerful catalytic materials for environmental pollutant detection and degradation

“…In this regard, several studies have been performed to fabricate optical approaches to achieve analysis of phosphate ions. [67][68][69][70][71][72] For instance, Huang et al synthesized a Cu-NADH material as a laccase mimic with attractive catalytic activity for the color reaction between 4-aminoantipyrine and 2,4-dichlorophenol. 67 Introduction of phosphate ions (Pi) could strongly inhibit its laccase-like activity.…”

“…[67][68][69][70][71][72] For instance, Huang et al synthesized a Cu-NADH material as a laccase mimic with attractive catalytic activity for the color reaction between 4-aminoantipyrine and 2,4-dichlorophenol. 67 Introduction of phosphate ions (Pi) could strongly inhibit its laccase-like activity. A series of proof of concepts suggested that phosphate could competitively bind to the Cu active sites and lead to the destruction of the coordination between Cu 2+ and the reduced form of nicotinamide adenine dinucleotide (NADH), which further destroyed the nanozyme's structure and reduced its catalytic feature.…”