Colorimetric chemosensor for rapid detection of fluoroquinolone load in environmental water bodies, urine, and counterfeit drug testing

Pal, Tathagata; Mathai, Tennyson; Mukherji, Soumyo

doi:10.1016/j.biosx.2023.100384

Cited by 5 publications

(1 citation statement)

References 52 publications

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“…While enzyme-linked immunosorbent assays (ELISAs) eliminate the need for sample pretreatment and certain matrix interferences can be eliminated by washing between steps, ELISAs require a laboratory setting, technical training, and multiple steps. Compared with colloidal gold-labeled immunochromatography strip assays (ICSAs), the novel immunosensors need to be further validated for practical application in the market, although they have the advantages of low manufacturing cost, simple storage conditions, no/minimal sample preparation, and immediate operability [12,13]. ICSAs offer a simpler and more convenient solution, and previous studies demonstrated that this method is effective in detecting fluoroquinolones immunosorbent [14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

An immunochromatographic strip sensor for marbofloxacin residues

Yang,

Li,

Kwee

et al. 2024

PLoS ONE

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Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.

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