Colorimetric Determination of Hydroxyproline

Miyada, D. S.; Tappel, Al L.

doi:10.1021/ac60113a039

Cited by 67 publications

(16 citation statements)

References 5 publications

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“…). Previous studies have suggested that the amount of collagen present in a tissue can be indirectly determined by measuring the amount of hydroxyproline through an acid hydralization technique (Miyada & Tappel ). To date, the literature has focused on the decellularization of distinct tissues and organs.…”

Section: Introductionmentioning

confidence: 99%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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The aim of this study was to develop a method to generate multi-organ acellular matrices. Using a foetal sheep model have developed a method of systemic pulsatile perfusion via the umbilical artery which allows for simultaneous multi-organ decellularization. Twenty sheep foetuses were systemically perfused with Triton X-100 and sodium dodecyl sulphate. Following completion of the whole-body decellularization, multiple biopsy samples were taken from different parts of 21 organs to ascertain complete cell component removal in the preserved extracellular matrices. Both the natural and decellularized organs were subjected to several examinations. The samples were obtained from the skin, eye, ear, nose, throat, cardiovascular, respiratory, gastrointestinal, urinary, musculoskeletal, central nervous and peripheral nervous systems. The histological results depicted well-preserved extracellular matrix (ECM) integrity and intact vascular structures, without any evidence of residual cellular materials, in all decellularized bioscaffolds. Scanning electron microscope (SEM) and biochemical properties remained intact, similar to their age-matched native counterparts. Preservation of the collagen structure was evaluated by a hydroxyproline assay. Dense organs such as bone and muscle were also completely decellularized, with a preserved ECM structure. Thus, as shown in this study, several organs and different tissues were decellularized using a perfusion-based method, which has not been previously accomplished. Given the technical challenges that exist for the efficient generation of biological scaffolds, the current results may pave the way for obtaining a variety of decellularized scaffolds from a single donor. In this study, there have been unique responses to the single acellularization protocol in foetuses, which may reflect the homogeneity of tissues and organs in the developing foetal body.

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Section: Introductionmentioning

confidence: 99%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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“…To estimate the total lung collagen content, the hydroxyproline content was measured according to an established method with modifications in five or 11 animals in each group on day 14 [20]. In brief, the whole lung from each animal was dissected free of the major bronchi.…”

Section: Methodsmentioning

confidence: 99%

ICAM-1 mediates lung leukocyte recruitment but not pulmonary fibrosis in a murine model of bleomycin-induced lung injury

Matsuse¹,

Teramoto²,

Katayama³

et al. 1999

Eur Respir J

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Bleomycin-induced lung injury has been extensively used as a model of interstitial pneumonia and pulmonary fibrosis. Intercellular adhesion molecule (ICAM)-1 is a ligand for lymphocyte function-associated antigen (LFA)-1alpha and has been shown to be required for leukocyte migration into inflamed areas. The purpose of this report was to investigate the role of the ICAM-1/LFA-1alpha pathway in a murine model of bleomycin-induced lung injury. Animals received 75 mg x kg(-1) bleomycin (BLM) i.v. followed by treatment with phosphate-buffered saline (BLM group), anti-ICAM-1 and LFA-1alpha monoclonal antibodies (mAb) (BLM+mAb group). Inflammatory cell counts of bronchoalveolar lavage (BAL) fluid, hydroxyproline content and histological findings were compared between these groups. In the BLM group, significant increases in total cell count, macrophage count and neutrophil count of BAL fluid were observed on days 7 and 14. In the BLM+mAb group, bleomycin-induced accumulation of neutrophils was significantly reduced on days 7 and 14 (p<0.01). However, the administration of mAb to ICAM-1 and LFA-1alpha did not decrease the lung hydroxyproline content or the histopathological fibrosis grading score, indicating that the antagonism of ICAM-I and LFA-1alpha did not attenuate bleomycin-induced pulmonary fibrosis. This study suggests that the intercellular adhesion molecule-1/lymphocyte function-associated antigen-1alpha pathway mediates the accumulation of inflammatory cells in the injured lung caused by bleomycin; however, other mechanisms are important for the subsequent development of pulmonary fibrosis.

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“…After weighing, the ash was dissolved in 6N-HCI solution to determine calcium and phosphorus content. The hydroxyproline (HOP) content of the femur was measured by the Miyada and Tappel method (23). The significance of the differences was tested by using Student's t test.…”

Section: Methodsmentioning

confidence: 99%

Immobilization osteoporosis and active vitamin D: Effect of active vitamin D analogs on the development of immobilization osteoporosis in rats

et al. 1981

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The therapeutic effects of vitamin D analogs, 1,24(R)-dihydroxycholecalciferol [1,24(R)-(OH)2D3], 1,24(S)-dihydroxycholecalciferol [1,24-(S)(OH)2D3], and 1,25-dihydroxycholecalciferol [1,25(OH)2D3] on immobilization osteoporosis were studied in rats. The right hind limb was immobilized through application of a plaster cast following the section of the sciatic nerve. The left hind limb was intact. Vitamin D analogs were orally administered for 6 weeks at dose levels of 0.02 and 0.10 micrograms/kg/day, respectively. The mean lengths of the immobilized femurs were not significantly different from those of the intact femurs in all the experimental groups. In the immobilized femur of animals treated with 1,24(R)(OH)2D3, 0.10 micrograms/kg, dry and ash weights were heavier and calcium and phosphorus contents greater than those in the nontreated group. Furthermore, the amount of calcified bone mass and the cortical thickness of the femurs of the immobilized limb in 1,24(OH)2D3-treated animals were greater than those in the nontreated animals. Treatment with 1,25(OH)2D3 at 0.10 micrograms/kg caused an increase of the bone mass in both immobilized and intact femurs when compared with those of the control group. It was concluded that the administration of 1,24(R)(OH)2D3 diminished the effect of immobilization in the development of osteoporosis without any side effects.

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Colorimetric Determination of Hydroxyproline

Cited by 67 publications

References 5 publications

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

ICAM-1 mediates lung leukocyte recruitment but not pulmonary fibrosis in a murine model of bleomycin-induced lung injury

Immobilization osteoporosis and active vitamin D: Effect of active vitamin D analogs on the development of immobilization osteoporosis in rats

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