Colorimetric differentiation of hexosamines

Ludowieg, Julio; Benmaman, Joseph D.

doi:10.1016/0003-2697(67)90136-4

Cited by 136 publications

(41 citation statements)

References 9 publications

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“…Neutral sugar analysis of the EPS was performed by GC as described by Fox et al (1989). The aminosugar content of the EPS was analysed by the method of Ludowieg & Benmaman (1967).…”

Section: Methodsmentioning

confidence: 99%

The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide

et al. 2000

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The cpsFGHIJKL genes from the cps cluster of Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of EPS were identified, cloned and nucleotide sequenced. The complete cps cluster is contained on an " 11 2 kb chromosomal region which contains 12 ORFs, including the previously cloned cpsABCDE genes. Functions were assigned to some of the predicted gene products on the basis of homology to known sequences as follows : cpsK encodes a protein thought to be involved in the polymerization and export of the polysaccharide ; cpsE, cpsF, cpsG, cpsH, cpsI and cpsJ encode putative sugar transferases. Two insertion sequences, IS1193 and ISS1, were identified within and flanking the 3' end of the cps cluster respectively. Analysis of the expression of the cpsE gene in Escherichia coli demonstrated that it encodes a glucose-1-phosphate transferase ; the enzyme which catalyses the first step in EPS biosynthesis in S. thermophilus NCFB 2393.

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“…Neutral sugar analysis of the EPS was performed by GC as described by Fox et al (1989). The aminosugar content of the EPS was analysed by the method of Ludowieg & Benmaman (1967).…”

Section: Methodsmentioning

confidence: 99%

The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide

et al. 2000

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“…Hexosamine was determined (Ludowieg & Benmaman 1967) after hydrolysis for 14 hours in 4 M hydrochloric acid at 100OC. Uronic acid was assayed with m-hydroxydiphenyl using Dglucuronic acid as a standard (Blumenkrantz & Asboe-Hansen 1973).…”

Section: Methodsmentioning

confidence: 99%

Glycosaminoglycan Metabolism in Experimental Osteoarthrosis Caused by Immobilization

Eronen¹,

Videman²,

Friman³

et al. 1978

Acta Orthopaedica Scandinavica

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“…,B-D-GNAc (1 -N)Asn andp-n-Gal All sugars except N-acetylneuraminic acid were determined by gas/liquid chromatography as their alditol acetates (19). Hexosamines were also determined colorimetrically (20)(21)(22). N-Acetylneuraminic acid was quantified according to Warren (23).…”

Section: Methodsmentioning

confidence: 99%