Colorimetric immunocapture loop mediated isothermal amplification assay for detection of Impatiens necrotic spot virus (INSV) by GineFinder™ dye

Almasi, Mohammad; Almasi, Galavizh

doi:10.1007/s10658-017-1294-6

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…The fundamental issue in controlling viral disease is that diseases are difficult to cure, and viruses have no cure; therefore, the only choice is to evade infection 16, 17 . Sowing resistant cultivars, and the use of insecticides to remove virus vectors and herbicides to destroy the pathogen's alternate hosts are essential techniques for ZYMV control 18 .…”

Section: Introductionmentioning

confidence: 99%

Copper oxide nanostructures as a potential method for control of zucchini yellow mosaic virus in squash

Derbalah

Abdelsalam

Behiry

et al. 2022

Pest Management Science

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BACKGROUND: Zucchini yellow mosaic virus (ZYMV) infects cucurbits and has been identified as a major limiting factor in their production. The purpose of this study was to create copper oxide nanostructures (CONS) to control ZYMV in squash plants. Protection of squash against ZYMV was assessed in terms of virus severity, ZYMV concentration, transcription of pathogenesisrelated genes and growth enhancement of treated squash. RESULTS:The findings revealed that squash plants treated with CONS had a significant reduction in disease severity when compared with untreated plants. In squash plants treated with CONS, defense genes associated with the salicylic acid signaling pathway were strongly expressed compared with untreated plants. The structural characteristics of CONS, such as their small size and appropriate shape, added to their excellent anti-ZYMV efficacy. CONS-treated squash plants show significantly improved growth traits compared with untreated plants.CONCLUSION: Based on the results of this study, CONS may be a new strategy for the control of ZYMV in squash. This represents an unconventional solution to control this virus, particularly as no chemical pesticides can control viral diseases.

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Section: Introductionmentioning

confidence: 99%

Copper oxide nanostructures as a potential method for control of zucchini yellow mosaic virus in squash

Derbalah

Abdelsalam

Behiry

et al. 2022

Pest Management Science

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“…Under favorable circumstances for the pathogen, the symptoms that viral infections can cause are mottling, drying of leaves, malformation of fruit, necrosis, plant stunting, and even death [9]. The main problem in the control of diseases is that they are challenging to cure, and in the case of viruses, there is no cure; the only options are to prevent or avoid infection [17,18]. Currently, nanoscale materials have emerged as novel antibacterial, antifungal, and antiviral compounds for use in animals and, to a lesser extent, in plants [9,19,20].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles as Potential Antivirals in Agriculture

et al. 2020

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Viruses are estimated to be responsible for approximately 50% of the emerging plant diseases, which are difficult to control, and in some cases, there is no cure. It is essential to develop therapy practices to strengthen the management of these diseases caused by viruses in economically important crops. Metal nanoparticles (MeNPs) possess diverse physicochemical properties that allow for them to have a wide range of applications in industry, including nanomedicine and nano-agriculture. Currently, there are reports of favorable effects of the use of nanoparticles, such as antibacterial, antifungal, and antiviral effects, in animals and plants. The potential antiviral property of MeNPs makes them a powerful option for controlling these histological agents. It is crucial to determine the dosage of NPs, the application intervals, their effect as a biostimulant, and the clarification of the mechanisms of action, which are not fully understood. Therefore, this review focuses on discussing the ability of metal nanoparticles and metal oxides to control viruses that affect agriculture through an exhaustive analysis of the characteristics of the particles and their interaction processes for a possibly beneficial effect on plants.

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“…There is an increasing interest in utilizing biocontrol agents as eco-friendly substitutes for harmful pesticides in plant pest control to enhance long-term sustainability in agriculture and the environment [11,12]. Diseases, such as viruses, provide a challenge in treatment, making prevention the most effective approach [13]. Currently, nanoscale materials have been produced to act as antiviral, antifungal, and antibacterial agents for animals and, to a lesser degree, for plants [14,15].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesized silver nanoparticles mediated by Ammi visnaga extract enhanced systemic resistance and triggered multiple defense-related genes, including SbWRKY transcription factors, against tobacco mosaic virus infection

Aseel,

Ibrahim,

Abdelkhalek

2024

Preprint

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Background Tobacco mosaic virus (TMV) is a highly infectious plant virus that impacts a broad variety of plants and reduces crop yield around the world. Here, we assessed the effectiveness of using Ammi visnaga aqueous extract to synthesize silver nanoparticles (Ag-NPs) and their potential to combat TMV. Different techniques were used to characterize Ag-NPs, such as scanning and transmission electron microscopy (SEM, TEM), energy-dispersive X-ray spectroscopy (EDS), fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). Results TEM demonstrated that the biosynthesized Ag-NPs had a spherical form with an average size of 25 nm. The zeta potential indicated the high stability of Ag-NPs, with a value of -15.9 mV. FTIR revealed various functional groups involved in the stability and capping of Ag-NPs. Interestingly, the Pre-treatment of tobacco plants (protective treatment) with Ag-NPs at 100 or 500 µg/mL significantly suppressed viral symptoms, while the Post-treatment (curative treatment) delayed their appearance. Furthermore, protective and curative treatments significantly increased chlorophyll a and b, total flavonoids, total soluble carbohydrates, and antioxidant enzymes activity. At the same time, it caused a drop in oxidative stress markers. The RT-qPCR results and volcano plots analysis showed that the Ag-NPs treatments triggers and regulates transcriptional of ten defense-related genes (SbWRKY-1, SbWRKY-2, JERF-3, GST-1, POD, PR-1, PR-2, PR-12, PAL-1, HQT-1). The heatmap revealed that GST-1, the primary gene involved in anthocyanidin production, was consistently the most expressed gene across all treatments throughout the study. Analysis of the gene co-expression network revealed that SbWRKY-19 was the most central gene among the studied genes, followed by PR-12 and PR-2. Conclusions Overall, the reported antiviral properties (protective and/or curative) of biosynthesized Ag-NPs against TMV lead us to recommend using Ag-NPs as a simple, stable, and eco-friendly agent in developing pest management programs against plant viral infections.

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Colorimetric immunocapture loop mediated isothermal amplification assay for detection of Impatiens necrotic spot virus (INSV) by GineFinder™ dye

Cited by 10 publications

References 25 publications

Copper oxide nanostructures as a potential method for control of zucchini yellow mosaic virus in squash

Copper oxide nanostructures as a potential method for control of zucchini yellow mosaic virus in squash

Nanoparticles as Potential Antivirals in Agriculture

Biosynthesized silver nanoparticles mediated by Ammi visnaga extract enhanced systemic resistance and triggered multiple defense-related genes, including SbWRKY transcription factors, against tobacco mosaic virus infection

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