Colour changes in Labeo rohita (Ham.) due to pigment translocation in melanophores, on exposure to municipal wastewater of Tung Dhab drain, Amritsar, India

Kaur, Rajbir; Dua, Anish

doi:10.1016/j.etap.2015.01.007

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…The wastewater samples like total suspended solids (TSS), biological oxygen demand (BOD), nitrate as N (NO 3 -N), oil and grease (O and G) and heavy metals like chromium Cr (VI), manganese (Mn) and lead (Pb) did not meet the discharge standards of effluents into inland surface waters as previously described in one of our manuscript (Kaur and Dua 2015c). The recorded values showed deteriorated water quality of Tung Dhab drain, hence supporting the higher incidence of MN, BN and NA in the liver, gill and erythrocytes in the experimental fish.…”

Section: Resultsmentioning

confidence: 63%

Fish liver and gill cells as cytogenotoxic indicators in assessment of water quality

Kaur

Dua

2016

Environ Sci Pollut Res

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The present study was aimed to evaluate the effect of municipal wastewater of Tung Dhab drain to freshwater fish Labeo rohita through genotoxicity assays. The idea is to explore and standardize procedures for assessment of toxic impact of pollutant at the cellular level in aquatic species. Hence, chronic, non-renewal toxicity tests were performed with sublethal concentrations (17.7, 26.6 and 35.4 %) of wastewater for exposure durations of 15, 30 and 60 days. Recovery experiments were also performed for duration of 60 days. Higher micronuclei (MN) and binuclei (BN) frequencies were recorded in fluorescently stained liver cells as compared to frequencies obtained for gill cells and erythrocytes. The induced frequencies of MN, BN and nuclear abnormalities (NA) were found in the following order: MN (liver cells) > BN (liver cells) > BN (gill cells) > MN (gill cells) > NA (erythrocytes) > MN (erythrocytes). The results pointed towards the mutagenic nature of water quality of Tung Dhab drain and recommend the incorporation of the liver and gill cells as biomarkers of cytotoxicity over erythrocytes in chronic experiments during water monitoring programs.

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Section: Resultsmentioning

confidence: 63%

Fish liver and gill cells as cytogenotoxic indicators in assessment of water quality

Kaur

Dua

2016

Environ Sci Pollut Res

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“…As per Gleeson et al (2012), the world's groundwater supplies are rapidly decreasing, most prominently in Asia and North America. Worldwide, surface waters are deteriorating due to unregulated discharge of industrial effluents, agricultural run-off, and domestic sewage (Kaur and Dua, 2015a, 2015b, 2015c. Wastewater management in India has become extremely important due to health concerns and population pressure.…”

Section: Introductionmentioning

confidence: 99%

Induction of histopathological lesions in renal tissue of the fish Labeo rohita upon exposure to municipal wastewater of Tung Dhab Drain, Amritsar, India

Kaur¹,

Dua²

2016

Turk J Zool

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Labeo rohita were subjected to chronic (15, 30, and 60 days) and recovery (60 days) experiments for kidney histopathology studies using sublethal concentrations (35.4%, 26.6%, and 17.7%) of municipal wastewater collected from Tung Dhab Drain, Amritsar, India. Control fish were maintained simultaneously using laboratory tap water, and exhibited normal structures. The results exhibited both dose-and duration-dependent alterations in exposed tissues. The mean degree of tissue change (DTC) values in treated groups ranged from 37.5 ± 6.60 (17.7%, 15 days) to 149.33 ± 7.75 (35.4%, 60 days), demonstrating reparable to irreparable structural changes. The recorded observations showed changes in tubules that included nuclear and cellular hypertrophy, cytoplasmic vacuolation, narrowing of the tubular lumen (stage I), hyaline droplet degeneration, occlusion of the tubular lumen (stage II), and tubular necrosis (stage III). Clear damage was also witnessed in corpuscles that included enlargement of glomerulus, dilatation of glomerular capillaries (stage I), and reduction of Bowman's space (stage II). The recovery experiments showed statistically significant improvement, as the intensity of damage was less than that recorded after 60 days of treatment. The present study indicates toxicity of drain water at the cellular and organ levels, and recommends the use of kidney histopathology as an analytical tool in toxicology and water surveillance studies.

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“…Moreover, the development of melanocytes is controlled by many factors and these factors cooperate with each other to form a gene network that regulates the development and differentiation of melanocytes derived from neural crests (Setty et al, 2007;Mort et al, 2015). The TYR is a key and rate-limiting enzyme that controls melanin synthesis, the low expression level of TYR may directly and closely relate to the albinism of the skin (Koga and Hori, 1997;Li et al, 2014; Hirobe and Ishikawa, 2015; Kaur and Dua, 2015;Jiang et al, 2019). Meanwhile, the MITF is an upstream TF that can directly regulate the TYR gene (Bharti et al, 2012;Raviv et al, 2014), and some genes such as SOX10, MAPK, CATs act directly or indirectly on the MITF gene to regulate the development, growth and differentiation melanocytes (Hou et al, 2000).…”

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confidence: 99%

Insights Into the Albinism Mechanism for Two Distinct Color Morphs of Northern Snakehead, Channa argus Through Histological and Transcriptome Analyses

et al. 2020

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The great northern snakehead (Channa argus) is one of the most important economic and conservational fish in China. In this study, the melanocytes in the skin of two distinct color morphs C. argus were investigated and compared through employment of the microscopic analysis, hematoxylin and eosin (H&E) and Masson Fontana staining. Our results demonstrated the uneven distribution of melanocytes with extremely low density and most of them were in the state of aging or death. Meanwhile, there was no obvious pigment layer and melanocytes distribution pattern found in the albino-type (AT), while the melanocytes were evenly distributed with abundance in the bicolor-type (BT). The transcriptome analysis through Illumina HiSeq sequencing showed that a total of 34.93 Gb Clean Data was obtained, and Q30 base percentage reached 92.66%. The BT and AT northern snakeheads transcriptome data included a total of 56,039,701 and 60,410,063 clean reads (n = 3), respectively. In gene expression analyses, the sample correlation coefficients (r) were ranged between 0.92 and 1.00; the contribution of PC1 and PC2 were 50.25 and 13.73% by using PCA cluster analysis, the total number of DEGs were 1024 (559 up-regulated and 465 down-regulated), and the number of annotated DEGs was 767 (COG 172, KEGG 262, GO 288, SwissProt 548, Pfam 579 and NR 765). Additionally, 46,363 ± 873 and 44,947 ± 392 single nucleotide polymorphisms (SNPs) were compiled via genetic structure analysis, respectively. Ten key pigment-related genes were screened using qRT-PCR. And all of them revealed extremely higher expression levels in the skin of BT than those of AT.This is the first study to analyze the mechanism of albino characteristics of Channa via histology and transcriptomics, and also provide the oretical and practical support for the protection and development of germplasm resources for C. argus.

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Colour changes in Labeo rohita (Ham.) due to pigment translocation in melanophores, on exposure to municipal wastewater of Tung Dhab drain, Amritsar, India

Cited by 6 publications

References 28 publications

Fish liver and gill cells as cytogenotoxic indicators in assessment of water quality

Fish liver and gill cells as cytogenotoxic indicators in assessment of water quality

Induction of histopathological lesions in renal tissue of the fish Labeo rohita upon exposure to municipal wastewater of Tung Dhab Drain, Amritsar, India

Insights Into the Albinism Mechanism for Two Distinct Color Morphs of Northern Snakehead, Channa argus Through Histological and Transcriptome Analyses

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