Colour‐Doppler ultrasound imaging as a laparoscopy substitute to count corpora lutea in superovulated sheep

Pinto, Pedro Henrique Nicolau; Bragança, G. M.; Balaro,; Arashiro, E. K.; Santos, GB dos; Souza, GN de; Souza-Fabjan, J. M. G.; Fonseca, JF; Brandão, F. Z.

doi:10.1111/rda.13089

Cited by 15 publications

(6 citation statements)

References 9 publications

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“…In most sheep MOET programmes, standard methods for evaluating the superovulatory response, collecting and transferring embryos are still performed by surgical methods. However, efforts have been made to replace these procedures and allow MOET to become totally non‐surgical (Fonseca, Oliveira, Brandão, Batista, et al, 2019; Pinto et al., 2018; Wulster‐Radcliffe et al., 1999). In short, this study presents the relevant findings that a CDP based only on oxytocin is efficient and safe for the CL and that a screening test for NSET is useful.…”

Section: Discussionmentioning

confidence: 99%

Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep

Santos

Pinto

Balaro

et al. 2020

Reprod Domestic Animals

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Because of the features of the ovine cervix, including a narrow lumen and misaligned folds, access to the uterine lumen through the cervical canal is particularly challenging in this species (Halbert, Dobson, Walton, & Buckrell, 1990). Therefore, surgical procedures are commonly applied in assisted reproductive technologies in sheep when access to the uterine lumen is needed (Candappa & Bartlewski, 2011, 2014). Surgical intervention is not desired in assisted reproductive technologies because it can lead to the formation of adhesions in the reproductive tract (up to 30%) (Bruno-Galarraga et al., 2014; Pinto et al., 2020) and, consequently, reproductive impairment.

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Section: Discussionmentioning

confidence: 99%

Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep

Santos

Pinto

Balaro

et al. 2020

Reprod Domestic Animals

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“…Finally, from a practical point of view, the synchronized oestrous induction plus eCG single‐shot allows to evaluate by US the donor potential to promote multiple ovulations (Bruno‐Galarraga et al., 2015), as well as simulate all stages of NSER following cervical dilation treatment in synchronized ewes (Figueira et al., 2020). The US is also a powerful tool to measure the efficiency in multiple ovulation and embryo transfer (MOET) programmes based on NSER by CL counting (Oliveira et al., 2018; Pinto et al., 2018). Likewise, ultrasonographic cervical evaluation can also become a valuable tool to classify and select eligible animals for NSER.…”

Section: Discussionmentioning

confidence: 99%

Ultrasonographic cervical evaluation: A tool to select ewes for non‐surgical embryo recovery

Figueira

Alves

Souza-Fabjan

et al. 2020

Reprod Domestic Animals

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Cervical anatomy in sheep is the major limitation for the application of reproductive biotechnologies by non-surgical methods (Fonseca et al., 2016; Kershaw et al., 2005) and is highly variable among breeds (Kaabi et al., 2006). The successful cervical transposing is affected by complex anatomy of the cervical lumen and the difficulty of rectal manipulation of the reproductive tract. Furthermore, cervices with higher degree of convolutions and interdigitations are less penetrable by pipette/catheter (Kershaw et al., 2005). Pharmacological induction of cervical dilation is a crucial point to efficiently perform non-surgical embryo recovery (NSER) in sheep

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“…Goats were mated with fertile bucks with a ratio of 4:1. At the first day of the protocol (D0) and six days after oestrus onset (D13), transrectal US evaluations were done to observe ovarian status and estimate the number of CLs,25 respectively. Seven days after oestrus onset, NSER was performed26 (figure 1).…”

Section: Methodsmentioning

confidence: 99%

Embryo development is impaired in goats that are treated for hydrometra and subsequently subjected to superovulation

Maia¹,

Arrais²,

Prellwitz³

et al. 2020

Veterinary Record

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BackgroundReproductive efficiency after hydrometra (HD) treatment is usually unsatisfactory.MethodsTo identify mechanisms involved in low reproductive efficiency of HD-treated goats, pluriparous dairy goats treated for HD (n=10, HD) or with no reproductive disorders (n=11, control: CONT) were induced to oestrus and superovulated. Goats were mated with fertile bucks and seven days after oestrus, non-surgical embryo recovery was performed. Embryos were evaluated and gene expression was performed.ResultsThere were no differences (P>0.05) in sexual behaviour parameters, superovulation response, mean number of retrieved structures and viable embryos between groups; although embryo recovery rate was higher (P=0.01) in CONT group. Structures in delayed stage (8–16 cells) were more frequent (P<0.05) in HD (29 vs 1 per cent) goats, as well as the percentage of advanced embryos was greater (P<0.05) for CONT (59.3 vs 33.3 per cent) goats. However, the expression of genes related to apoptosis (BAX and Bcl-2), trophectoderm differentiation (CDX2) and pluripotency maintenance (NANOG) was not affected (P>0.05) in embryos that reached the morulae and blastocyst stages.ConclusionAlthough the HD embryos that developed to morula and blastocyst stages showed no change in the expression of genes related to their quality and implantation capacity, overall, embryo development was impaired in HD-treated goats.

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Colour‐Doppler ultrasound imaging as a laparoscopy substitute to count corpora lutea in superovulated sheep

Cited by 15 publications

References 9 publications

Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep

Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep

Ultrasonographic cervical evaluation: A tool to select ewes for non‐surgical embryo recovery

Embryo development is impaired in goats that are treated for hydrometra and subsequently subjected to superovulation

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