Combination of synchrotron radiation-based Fourier transforms infrared microspectroscopy and confocal laser scanning microscopy to understand spatial heterogeneity in aquatic multispecies biofilms

Investigation of corrosion inhibitory process of marine Vibrio neocaledonicus sp. bacterium for carbon steel

“…The peaks at 1639.9 cm −1 and 1493.6 cm −1 are related to carboxylic anion and C=O of amide I [19]. The amide presence likely corresponds to secreted proteins in the biofilm [20]. Two other major peaks are detected in 1130.4 cm −1 and 854.4 cm −1 related both to carbohydrate and C-O stretching in esters [10].…”

Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review

“…Spectroscopic techniques are well-established techniques for identifying functional groups in molecules. They are of outstanding importance for online, non-invasive biofilm monitoring, especially when coupled with for spectral calibration and pattern recognition (Reuben et al 2014). Furthermore, spectroscopic techniques could be used to qualitatively and quantitatively analyze EPS compositions.…”

“…Spectral fingerprints are then obtained, with the contributions of the functional groups of all biochemical molecules in the sample combined. Samples must be dried before FTIR analysis because of the strong absorption of water in the mid-IR region (Reuben et al 2014).…”

“…Therefore, NMR is considered a relatively insensitive method compared with optical methods. Furthermore, NMR for the proton resonance requires labelled substrates by using isotope or non-isotope, and the label-requiring technologies may affect the biofilm physiology (Reuben et al 2014).…”

“…The individual spectral features of FTIR often overlap because of the extreme heterogeneity of biofilm constituents. Consequently, ATR-FTIR is suitable for analyzing the EPS extracted from biofilms (Reuben et al 2014). Notably, the interpretation of spectral changes measured at the molecular level is sometimes subtle and complex, requiring knowledge and experience of ATR-FTIR bacterial fingerprints to be able to identify and differentiate the spectral changes induced by changes in environmental conditions (Humbert and Quilès 2011).…”