Combination therapy with anti-CD20 mAb and IL-10 gene to reverse type 1 diabetes by attenuating pancreatitis and inhibiting apoptosis in NOD mice

Cheng, Li; Zhang, Lijuan; Qiao, Lingyan; Hu, Sicui; Ge, Juan; Hu, Conghui; Tang, Li

doi:10.1016/j.lfs.2020.117985

Cited by 6 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…43 In contrast, gene IL-10 inhibits the activity of caspase, changes the ratio of bax/bcl-2, and blocks the Fas/FasL pathway, thereby reducing the IL-1β-mediated apoptosis of pancreatic β cells. 44 As shown in Fig. 3A-C, compared with the NFD group, the serum concentrations of IL-6 and TNF-α significantly increased ( p < 0.001).…”

Section: Effects Of Svm Intervention On the Levels Of Inflammatory Fa...mentioning

confidence: 75%

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Huang

Liu

et al. 2022

Food Funct.

View full text Add to dashboard Cite

show abstract

Section: Effects Of Svm Intervention On the Levels Of Inflammatory Fa...mentioning

confidence: 75%

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Huang

Liu

et al. 2022

Food Funct.

View full text Add to dashboard Cite

show abstract

“…5,7,8 Post hoc analysis and real-world studies now show a sustained efficacy over a period of up to 7 years 9 and position OCRE and other anti-CD20 treatments as high-efficacy DMTs with a similar profile as natalizumab (NTZ). 10 At a mechanistic level, after binding CD20-expressing B cells, OCRE induces complement-dependent and antibody-dependent cellular cytotoxicity, 11 effectively depleting CD20 + and CD19 + cells 2 weeks after treatment initiation. 7,[12][13][14] CD20 is expressed with increasing concentrations from pre-B cells to naive and memory B cells.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Ocrelizumab Impairs the Phenotype and Function of Memory CD8 ⁺ T Cells

Mathias

Pantazou

Perriot

et al. 2023

Neurol Neuroimmunol Neuroinflamm

View full text Add to dashboard Cite

Background and ObjectiveDepleting CD20+B cells is the primary mechanism by which ocrelizumab (OCRE) is efficient in persons with multiple sclerosis (pwMS). However, the exact role of OCRE on other immune cell subsets directly or indirectly remains elusive. The purpose of this study is to characterize the dynamics of peripheral immune cells of pwMS on OCRE.MethodsWe collected blood samples from 38 pwMS before OCRE onset (T0) and at 6 and 12 months (T6, T12) after initiation. To cover the immune cell diversity, using mass cytometry time of flight, we designed a 38-parameter panel to analyze B, T, and innate immune cell markers and CNS migratory markers. In parallel, viral-specific CD8+T-cell responses were assessed by the quantification of interferon-γ secretion using the enzyme-linked immunospot assay on cytomegalovirus, Epstein-Barr virus, and influenza stimulations.ResultsBeside B-cell depletion, we observed a loss in memory CD8+CD20+and central memory CD8+T cells but not in CD4+CD20+T cells already at T6 and T12 (p< 0.001). The loss of memory CD8+T cells correlated with a lower CXCR3 expression (p< 0.001) and CNS-related LFA-1 integrin expression (p< 0.001) as well as a reduced antiviral cellular immune response observed at both time points (p< 0.001). Of note, we did not observe major changes in the phenotype of the other cell types studied. Seven of 38 (18.4%) patients in our cohort presented with infections while on OCRE; 4 of which were switched from dimethyl fumarate. Finally, using a mixed linear model on mass cytometry data, we demonstrated that the immunomodulation induced by previous disease-modifying therapies (DMTs) was prolonged over the period of the study.DiscussionIn addition to its well-known role on B cells, our data suggest that OCRE also acts on CD8+T cells by depleting the memory compartment. These changes in CD8+T cells may be an asset in the action of OCRE on MS course but might also contribute to explain the increased occurrence of infections in these patients. Finally, although more data are needed to confirm this observation, it suggests that clinicians should pay a special attention to an increased infection risk in pwMS switched from other DMTs to OCRE.

show abstract

“…Mediators of inflammation—TNF-α, IL-1β, the IL-6 family of cytokines, IL-18, and certain chemokines—are believed to be involved in the etiology of diabetes [ 45 ]. IL-6 is regarded as an important proinflammatory factor, and anti-IL-6 therapies have good clinical potential; their use may expand in the future [ 49 ]. In addition to immunoregulatory actions, IL-6 is thought to affect glucose homeostasis and metabolism directly and indirectly by acting on skeletal muscle cells, adipocytes, hepatocytes, pancreatic β-cells, and neuroendocrine cells [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Trehalose Activates Hepatic and Myocardial Autophagy and Has Anti-Inflammatory Effects in db/db Diabetic Mice

Короленко¹,

Ovsyukova²,

Bgatova

et al. 2022

Life

View full text Add to dashboard Cite

Db/db mice (carrying a mutation in the gene encoding leptin receptor) show autophagy suppression. Our aim was to evaluate the effect of autophagy inducer trehalose on liver and heart autophagy in db/db mice and to study inflammation dysregulation and the suitability of chitinases’ expression levels as diabetes markers. Thirty-eight male db/db mice and C57/BL mice (control) were used. The db/db model manifested inflammation symptoms: overexpression of TNF-α in the spleen and underexpression of IL-10 in the liver and spleen (cytokine imbalance). Simultaneously, we revealed decreased expression of chitotriosidase (CHIT1) and acid mammalian chitinase (CHIA) in the liver of db/db mice. CHIA expression in db/db mice is significantly lower only in the spleen. Trehalose treatment significantly reduced blood glucose concentration and glycated hemoglobin. Treatment of db/db mice by trehalose was followed by increased autophagy induction in the heart and liver (increased autolysosomes volume density studied by morphometric electron-microscopic method). Trehalose exerted beneficial cardiac effects possibly via increased lipophagy (uptake of lipid droplets). The autophagy activation by trehalose had several positive effects on the heart and liver of db/db mice; therefore, lipophagy activation seems to be a promising therapy for diabetes.

show abstract

Combination therapy with anti-CD20 mAb and IL-10 gene to reverse type 1 diabetes by attenuating pancreatitis and inhibiting apoptosis in NOD mice

Cited by 6 publications

References 39 publications

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Ocrelizumab Impairs the Phenotype and Function of Memory CD8 ⁺ T Cells

Trehalose Activates Hepatic and Myocardial Autophagy and Has Anti-Inflammatory Effects in db/db Diabetic Mice

Contact Info

Product

Resources

About

Combination therapy with anti-CD20 mAb and IL-10 gene to reverse type 1 diabetes by attenuating pancreatitis and inhibiting apoptosis in NOD mice

Cited by 6 publications

References 39 publications

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Sanghuangporus vaninii mixture ameliorated type 2 diabetes mellitus and altered intestinal microbiota in mice

Ocrelizumab Impairs the Phenotype and Function of Memory CD8 + T Cells

Trehalose Activates Hepatic and Myocardial Autophagy and Has Anti-Inflammatory Effects in db/db Diabetic Mice

Contact Info

Product

Resources

About

Ocrelizumab Impairs the Phenotype and Function of Memory CD8 ⁺ T Cells