Combinatorial Approach Using Caenorhabditis elegans and Mammalian Systems for Aging Research

Lee, Gee-Yoon; Sohn, Jooyeon; Lee, Seung-Jae

doi:10.14348/molcells.2021.0080

Cited by 9 publications

(7 citation statements)

References 83 publications

(107 reference statements)

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“…It was very important to explore the occurrence and mechanism of aging. C. elegans was an excellent model to study with aging due to its physical decline strikingly similar to humans [ 42 ]. Typical markers of aging-relative phenotype in C. elegans included the locomotion decline, feeding ability (pharyngeal pumping frequency), defecation ability (defecation interval), morphological changes, and the aging-associated genes [ 29 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Zhang

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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Nowadays, human beings are exposed to Cu in varieties of environmental mediums, resulting in health risks needing urgent attention. Our research found that Cu shortened lifespan and induced aging-related phenotypes of Caenorhabditis elegans (C. elegans). Transcriptomics data showed differential expression genes induced by Cu were mainly involved in regulation of metabolism and longevity, especially in fatty acid metabolism. Quantitative detection of free fatty acid by GC/MS further found that Cu upregulated free fatty acids of C. elegans. A mechanism study confirmed that Cu promoted the fat accumulation in nematodes, which was owing to disorder of fatty acid desaturase and CoA synthetase, endoplasmic reticulum unfolded protein response (UPRER), mitochondrial membrane potential, and unfolded protein response (UPRmt). In addition, Cu activated oxidative stress and prevented DAF-16 translocating into nuclear with a concomitant reduction in the expression of environmental stress-related genes. Taken together, the research suggested that Cu promoted aging and induced fat deposition and oxidative damage.

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Section: Discussionmentioning

confidence: 99%

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Zhang

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Because studies regarding immunosenescence in C. elegans are limited, identifying novel factors that delay or reverse immune aging is required to further understand the relationship between immunity and aging. In C. elegans, several signaling pathways, including autophagy, unfolded protein response, and DBL-1/TGF-β pathway, regulate immunity and aging, in addition to p38 MAPK and IIS pathways that are discussed here [10,11,15,44]. Therefore, studying the roles of all these pathways in the context of age-dependent changes in immunity will provide additional mechanistic insights into immunosenescence.…”

Section: Discussionmentioning

confidence: 99%

Immunosenescence in Caenorhabditis elegans

2022

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Immunosenescence is an age-dependent decline in immune functions and hallmark of aging in diverse species, ranging from invertebrates to mammals. However, identifying the factors responsible for immunosenescence is challenging because of the complexity of immune systems and aging in mammals. The roundworm Caenorhabditis elegans is suitable for understanding immunosenescence because of its simple immune system and rapid aging process. In this review, we discuss the advances in our understanding of immunosenescence in C. elegans. PMK-1/p38 mitogen-activated protein kinase (MAPK), SKN-1/NRF, and ZIP-10/bZIP transcription factor regulate immunosenescence through p38 MAPK and insulin/IGF-1 signaling pathways. Because these factors and pathways are evolutionarily conserved, the findings discussed in this review may help understand the mechanisms underlying immunosenescence and develop new treatment therapy for immunosenescence in humans.

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“…C. elegans is a versatile model with a powerful genetics due to many advantages such as easy lab maintenance, a large brood size with approximately 300 offspring within a reproductive cycle of only three days and the short lifespan of three weeks in a typical culture condition, and practically no ethical concerns ( Meneely et al, 2019 ). Therefore, C. elegans has been widely used to study various questions asking principles in biological sciences and human diseases ( Chung et al, 2020 ; Kim et al, 2019 ; Lee et al, 2021a ; Levine and Lee, 2020 ).…”

Section: Hervs In Transgenic Animal Modelsmentioning

confidence: 99%

Human Endogenous Retroviruses as Gene Expression Regulators: Insights from Animal Models into Human Diseases

Durnaoglu

Lee

Ahnn

2021

Molecules and Cells

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The human genome contains many retroviral elements called human endogenous retroviruses (HERVs), resulting from the integration of retroviruses throughout evolution. HERVs once were considered inactive junk because they are not replication-competent, primarily localized in the heterochromatin, and silenced by methylation. But HERVs are now clearly shown to actively regulate gene expression in various physiological and pathological conditions such as developmental processes, immune regulation, cancers, autoimmune diseases, and neurological disorders. Recent studies report that HERVs are activated in patients suffering from coronavirus disease 2019 (COVID-19), the current pandemic caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection. In this review, we describe internal and external factors that influence HERV activities. We also present evidence showing the gene regulatory activity of HERV LTRs (long terminal repeats) in model organisms such as mice, rats, zebrafish, and invertebrate models of worms and flies. Finally, we discuss several molecular and cellular pathways involving various transcription factors and receptors, through which HERVs affect downstream cellular and physiological events such as epigenetic modifications, calcium influx, protein phosphorylation, and cytokine release. Understanding how HERVs participate in various physiological and pathological processes will help develop a strategy to generate effective therapeutic approaches targeting HERVs.

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Combinatorial Approach Using Caenorhabditis elegans and Mammalian Systems for Aging Research

Cited by 9 publications

References 83 publications

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Integrating Transcriptomics and Free Fatty Acid Profiling Analysis Reveal Cu Induces Shortened Lifespan and Increased Fat Accumulation and Oxidative Damage in C. elegans

Immunosenescence in Caenorhabditis elegans

Human Endogenous Retroviruses as Gene Expression Regulators: Insights from Animal Models into Human Diseases

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