Combinatorial decolorization performance of Pycnoporus sanguineus MUCL 38531 sourced recombinant laccase/mediator systems on toxic textile dyes

Malcı, Koray; Gur, Gunseli Kurt; Tamerler, Candan; Karataş, Ayten Yazgan

doi:10.1007/s13762-022-04080-4

Cited by 5 publications

(4 citation statements)

References 57 publications

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“…The addition of an appropriate inducer to the medium, such as syringaldehyde, pcoumaric acid, ABTS (2,2 ′ -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), or lignocellulosic agro-wastes, has been considered an effective procedure for regulating and increasing laccase production from Pycnoporus spp. [41][42][43]. The 17-ethinyl estradiol molecule helped to increase the expression of laccase by P. sanguineus in crops using cupuaçu (Theobroma grandiflorum) residues [44].…”

Section: The Genus Pycnoporusmentioning

confidence: 99%

“…Around 80% of LG could be decolorized using violuric acid [87]. Laccase from P. sanguineus MUCL 38531 was able to decolorize the dyes Reactive Black (93.2%), Crystal Violet (90%), Basic Fuchsin (83.4%), and Congo Red (88.2%) [42]. The crude laccase extract from P. sanguineus CS43 was purified, and the laccase fractions (Lac-I and Lac-II) were used to remove the RBBR dye.…”

Section: Decolorization and Degradation Of Synthetic Dyes By Pycnopor...mentioning

confidence: 99%

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Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Cheute,

Uber,

dos Santos

et al. 2024

Biomass

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Substantial amounts of organo-pollutants, often persistent and toxic, are generated globally each year, posing a threat to soil, water, groundwater, and air. The pollutants encompass a wide range of substances from various sources, which include solid as well as liquid ones, such as landfill leachates and wastewaters. The compounds include paper and pulp mill byproducts, pharmaceuticals, diverse types of plastics, hydrocarbons, pigments, and dyes, as well as pesticides and insecticides. Fungal bioremediation stands out as a promising technology that uses the metabolic potential of fungi to eliminate or mitigate the impact of pollutants. Notably, species of the genus Pycnoporus exhibit significant capabilities for degrading a broad spectrum of toxic molecules. This degradation is facilitated by released ligninolytic enzymes, especially laccase, and cellular enzymes pertaining to the cytochrome P450 monooxygenase system. The laccase, which is overproduced by the genus Pycnoporus, is quite remarkable for its high redox potential. The objective of this review is to highlight the proficiency of the Pycnoporus genus in the degradation of pollutants in submerged and solid-state fermentation. Recent studies conducted over the past decade consistently highlight the Pycnoporus genus as a robust contender in the realm of white biotechnology.

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Section: The Genus Pycnoporusmentioning

confidence: 99%

Section: Decolorization and Degradation Of Synthetic Dyes By Pycnopor...mentioning

confidence: 99%

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Cheute,

Uber,

dos Santos

et al. 2024

Biomass

View full text Add to dashboard Cite

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“…Microbial chassis have been extensively investigated for the sustainable and economically viable production of industrially important compounds, including pharmaceuticals [ 1 ], biofuels [ 2 ], enzymes [ 3 ] and polymers [ 4 ] in sustainable and economically attractive ways. Among these microbial hosts, Saccharomyces cerevisiae, commonly known as baker’s yeast, has emerged as the most widely studied eukaryotic synthetic biology chassis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Improved production of Taxol® precursors in S. cerevisiae using combinatorial in silico design and metabolic engineering

Malcı,

Santibáñez,

Jonguitud-Borrego

et al. 2023

Microb Cell Fact

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Background Integrated metabolic engineering approaches that combine system and synthetic biology tools enable the efficient design of microbial cell factories for synthesizing high-value products. In this study, we utilized in silico design algorithms on the yeast genome-scale model to predict genomic modifications that could enhance the production of early-step Taxol® in engineered Saccharomyces cerevisiae cells. Results Using constraint-based reconstruction and analysis (COBRA) methods, we narrowed down the solution set of genomic modification candidates. We screened 17 genomic modifications, including nine gene deletions and eight gene overexpressions, through wet-lab studies to determine their impact on taxadiene production, the first metabolite in the Taxol® biosynthetic pathway. Under different cultivation conditions, most single genomic modifications resulted in increased taxadiene production. The strain named KM32, which contained four overexpressed genes (ILV2, TRR1, ADE13, and ECM31) involved in branched-chain amino acid biosynthesis, the thioredoxin system, de novo purine synthesis, and the pantothenate pathway, respectively, exhibited the best performance. KM32 achieved a 50% increase in taxadiene production, reaching 215 mg/L. Furthermore, KM32 produced the highest reported yields of taxa-4(20),11-dien-5α-ol (T5α-ol) at 43.65 mg/L and taxa-4(20),11-dien-5-α-yl acetate (T5αAc) at 26.2 mg/L among early-step Taxol® metabolites in S. cerevisiae. Conclusions This study highlights the effectiveness of computational and integrated approaches in identifying promising genomic modifications that can enhance the performance of yeast cell factories. By employing in silico design algorithms and wet-lab screening, we successfully improved taxadiene production in engineered S. cerevisiae strains. The best-performing strain, KM32, achieved substantial increases in taxadiene as well as production of T5α-ol and T5αAc. These findings emphasize the importance of using systematic and integrated strategies to develop efficient yeast cell factories, providing potential implications for the industrial production of high-value isoprenoids like Taxol®.

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“…Microbial chassis have been intensely studied to produce industrially essential compounds such as pharmaceuticals [1], biofuels [2], enzymes [3] and polymers [4] in sustainable and economically attractive ways. Among these organisms, baker's yeast, Saccharomyces cerevisiae, is the most widely studied eukaryotic synthetic biology chassis [5].…”

Section: Introductionmentioning

confidence: 99%

Improved Production of Taxol® Precursors inS. cerevisiaeusing Combinatorialin silicoDesign and Metabolic Engineering

Malcı,

Santibáñez,

Jonguitud-Borrego

et al. 2023

Preprint

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Integrated metabolic engineering approaches combining system and synthetic biology tools allow the efficient designing of microbial cell factories to synthesize high-value products. In the present study, in silico design algorithms were used on the latest yeast genome-scale model 8.5.0 to predict potential genomic modifications that could enhance the production of early-step Taxol® in previously engineered Saccharomyces cerevisiae cells. The solution set containing genomic modification candidates was narrowed down by employing the COnstraints Based Reconstruction and Analysis (COBRA) methods. 17 genomic modifications consisting of nine gene deletions and eight gene overexpression were screened using wet-lab studies to determine whether these modifications can increase the production yield of taxadiene, the first metabolite in the Taxol® through the mevalonate pathway. Depending on the cultivation condition, most of the single genomic modifications resulted in higher taxadiene production. The best-performing strain, named KM32, contained four overexpressed genes, ILV2, TRR1, ADE13 and ECM31, from the branched-chain amino acid biosynthesis, thioredoxin system, de novo purine synthesis, and the pantothenate pathway, respectively. Using KM32, taxadiene production was increased by 50%, reaching 215 mg/L of taxadiene. The engineered strain also produced 43.65 mg/L of taxa-4(20),11-dien-α-ol (T5α-ol), and 26.2 mg/L of taxa-4(20),11-dien-5α-yl acetate (T5αAc) which are the highest productions of these early-step Taxol® metabolites reported until now in S. cerevisiae. The findings of this study highlight that the use of computational and integrated approaches can ensure determining promising modifications that are difficult to estimate intuitively to develop yeast cell factories.

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Combinatorial decolorization performance of Pycnoporus sanguineus MUCL 38531 sourced recombinant laccase/mediator systems on toxic textile dyes

Cited by 5 publications

References 57 publications

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Improved production of Taxol® precursors in S. cerevisiae using combinatorial in silico design and metabolic engineering

Improved Production of Taxol® Precursors inS. cerevisiaeusing Combinatorialin silicoDesign and Metabolic Engineering

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