2022
DOI: 10.3390/biom12070918
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Combined Analysis of BSA-Seq Based Mapping, RNA-Seq, and Metabolomic Unraveled Candidate Genes Associated with Panicle Grain Number in Rice (Oryza sativa L.)

Abstract: Rice grain yield is a complex and highly variable quantitative trait consisting of several key components, including the grain weight, the effective panicles per unit area, and the grain number per panicle (GNPP). The GNPP is a significant contributor to grain yield controlled by multiple genes (QTL) and is crucial for improvement. Attempts have been made to find genes for this trait, which has always been a challenging and arduous task through conventional methods. We combined a BSA analysis, RNA profiling, a… Show more

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Cited by 9 publications
(10 citation statements)
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“…The cetyltrimethylammonium bromide (CTAB) method was used to obtain high-quality genomic DNA [ 45 ]. DNA from 30 plants representing tolerance and sensitivity was equally mixed to form tolerance and sensitivity pools [ 25 ].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The cetyltrimethylammonium bromide (CTAB) method was used to obtain high-quality genomic DNA [ 45 ]. DNA from 30 plants representing tolerance and sensitivity was equally mixed to form tolerance and sensitivity pools [ 25 ].…”
Section: Methodsmentioning
confidence: 99%
“…This research indicates that natural genetic diversities provide valuable sources for identifying new candidate genes for breeding salt-tolerant crops. Recently, new strategies that combine several emerging technologies (Bulked Segregate Analysis, RNA-sequencing, metabolome analysis, or phosphoproteomic analysis) have been carried out to find new genes associated with several traits, such as the anthocyanin in cucumber ( Cucumis sativus L.) fruit skin [ 24 ], the panicle grain number in rice [ 25 ], and the plant architecture in Brassica napus [ 26 ].…”
Section: Introductionmentioning
confidence: 99%
“…The phenotypic characteristics of the DP15-CSSLs mature plants, including grain number (GN), TGW, GL, GW, GLWR, PH, LMC, AC, and LSC, were recorded under natural conditions in the experimental field during 2020 (from 20 November 2019 to 10 May 2020) in Sanya, China, and 2021 (from 2 March 2021 to 10 July 2021) in Nanning, Guangxi Province, China, respectively ( Figure 3 , Figures S2 and S3, and Table S5 ). When the seeds were matured under natural conditions, the dry seeds of each line were selected to measure the values of grain traits as previously described [ 47 ]. The tissue slices were processed by the frozen section machine Hestion CM2850.…”
Section: Methodsmentioning
confidence: 99%
“…Rice grain yield is a complex trait, which influenced by many other agronomic traits, such as the number of panicles per plant, grain numbers per panicle, grain weight, and grain filling ratio (Ma et al ., 2022). Grain size is one of the most important factors determining grain yield, appearance, and milling quality in rice.…”
Section: Introductionmentioning
confidence: 99%