Combined analysis of diffusion and relaxation behavior of water in apple parenchyma cells

Sibgatullin, T. A.; Jager, P.A. de; Vergeldt, Frank J.; Gerkema, E.; Анисимов, А. В.; As, Henk Van

doi:10.1134/s0006350907020091

Cited by 23 publications

(18 citation statements)

References 14 publications

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“…To date, NMR relaxometry has mainly been used for the characterization of fruit and vegetable tissues and has been shown to be effective in providing valuable information about cell organization (Sibgatullin et al, 2007). However, although a number of studies have contributed to the interpretation of the NMR results (Snaar and Van As, 1992;Hills and Nott, 1999;Marigheto et al, 2009), this is still not always straightforward, as the NMR signal depends both on the nature of the plant tissue and on the NMR measurement protocol.…”

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confidence: 95%

“…This is far beyond the relative intensity of component 2. On the other hand, the T2 values measured (Table II) may indicate that component 2 corresponds to the water in rapid exchange with cell wall protons according to the attribution proposed for fruit (Sibgatullin et al, 2007). Additionally, the relative water volume enclosed in the cell wall could correspond to the relative NMR signal intensity of component 2 (Fig.…”

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confidence: 99%

“…The T2 relaxation time is more sensitive to small variations in water content and chemical exchange processes than T1 and, therefore, is usually preferred. Indeed, differences in T1 for the different compartments are relatively small, resulting in an averaging effect that results in poor discrimination between water compartments (Van As, 2007).To date, NMR relaxometry has mainly been used for the characterization of fruit and vegetable tissues and has been shown to be effective in providing valuable information about cell organization (Sibgatullin et al, 2007). However, although a number of studies have contributed to the interpretation of the NMR results (Snaar and Van As, 1992;Hills and Nott, 1999;Marigheto et al, 2009), this is still not always straightforward, as the NMR signal depends both on the nature of the plant tissue and on the NMR measurement protocol.…”

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confidence: 99%

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Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

et al. 2013

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Nitrogen use efficiency is relatively low in oilseed rape (Brassica napus) due to weak nitrogen remobilization during leaf senescence. Monitoring the kinetics of water distribution associated with the reorganization of cell structures, therefore, would be valuable to improve the characterization of nutrient recycling in leaf tissues and the associated senescence processes. In this study, nuclear magnetic resonance (NMR) relaxometry was used to describe water distribution and status at the cellular level in different leaf ranks of well-watered plants. It was shown to be able to detect slight variations in the evolution of senescence. The NMR results were linked to physiological characterization of the leaves and to light and electron micrographs. A relationship between cell hydration and leaf senescence was revealed and associated with changes in the NMR signal. The relative intensities and the transverse relaxation times of the NMR signal components associated with vacuole water were positively correlated with senescence, describing water uptake and vacuole and cell enlargement. Moreover, the relative intensity of the NMR signal that we assigned to the chloroplast water decreased during the senescence process, in agreement with the decrease in relative chloroplast volume estimated from micrographs. The results are discussed on the basis of water flux occurring at the cellular level during senescence. One of the main applications of this study would be for plant phenotyping, especially for plants under environmental stress such as nitrogen starvation.The main physiological outcome of leaf senescence is the recycling of organic resources and the provision of nutrients to sink organs such as storage and growing tissues (Buchanan-Wollaston, 1997;Hikosaka, 2005;Krupinska and Humbeck, 2008). In crop plants, senescence progresses from the lower older leaves to the younger top leaves. Macromolecular degradation and the mechanism of reallocation of breakdown products are mediated by the up-regulation of senescence-related genes (Lee et al., 2001) in close relationship with both developmental and environmental conditions (Gombert et al., 2006). This leads to remobilization of carbon and nitrogen (N) compounds mostly from plastidial compartments (Martínez et al., 2008;Guiboileau et al., 2012), involving proteolytic activity in plasts, vacuole, and cytosol (Adam and Clarke, 2002;Otegui et al., 2005), chlorophyll breakdown (Hoertensteiner, 2006), galactolipid recycling (Kaup et al., 2002) in the plastoglobules (Brehelin et al., 2007), and loading of Suc and amino acids into the phloem through appropriate transporters (Wingler et al., 2004;Masclaux-Daubresse et al., 2008). In terms of leaf senescence at the cell level, where chloroplasts are degraded sequentially, relative organelle volume does not seem to be greatly modified, the vacuole remains intact, and in darkness-induced senescence the number of chloroplasts per cell decreases only slightly (Keech et al., 2007). However, major changes in metabolic fluxes and cell water...

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confidence: 99%

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Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

et al. 2013

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“…13 However, for the well-resolved target peak in the 1D sliced DOSY spectrum extracted with an SPLMOD algorism used in this study, the quantitative analysis would be appropriate, since the SPLMOD program automatically attempts to fit one-, two-or three-exponential attenuation functions to the data, and to evaluate the one that gives the best statistical fit, 11 by which the T2 value can also be sufficed to describe the spin-spin relaxation behavior. 21 With the aid of an IS, we tried to quantify the D-(+)-glucose content by the DOSY-qNMR method with the SPLMOD algorism, in which the 1D sliced DOSY spectra from the D value of 5.6 × 10 -10 m 2 /s were taken. In this study, D-glucuronic acid with a D value of 5.6 × 10 -10 m 2 /s was used as an IS candidate, which was produced at low concentrations (< 0.01 g/L) by the tea fungus, 22 but was not present in the fruit juices used in this study ( (Fig.…”

Section: Dosy-qnmr Analysis Of D-(+)-glucosementioning

confidence: 99%

Quantitative Analysis of D-(+)-Glucose in Fruit Juices Using Diffusion Ordered-1H Nuclear Magnetic Resonance Spectroscopy

et al. 2014

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“…The same component attributions were also proposed by Hills and Remigereau [8]. More recently, four components in T 2 were found in apple cells and were assigned to the vacuole, cytoplasm, extracellular space and cell wall [9,10]. However, this attribution and distribution does not necessarily hold for all types of plant tissue.…”

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confidence: 84%

NMR Study of Water Distribution inside Tomato Cells: Effects of Water Stress

2010

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Tomato pericarp tissue was studied by low field nuclear magnetic resonance (NMR) Relaxometry. Two kinds of experiment were performed in order to investigate the correlation between multi-exponential NMR relaxation and the subcellular compartments. Longitudinal (T 1 ) versus transverse (T 2 ) relaxation times were first measured on fresh samples and then the transverse relaxation time was measured on samples exposed to water stress. Four signal components were found in all experiments. The results showed that all signal components corresponded to the water in different cell compartments, and that no signal from non-exchangeable protons was present. Moreover, we demonstrated that NMR relaxation is suitable for the continuous monitoring of water rebalancing between subcellular compartments of plant tissues.

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Combined analysis of diffusion and relaxation behavior of water in apple parenchyma cells

Cited by 23 publications

References 14 publications

Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

Quantitative Analysis of D-(+)-Glucose in Fruit Juices Using Diffusion Ordered-1H Nuclear Magnetic Resonance Spectroscopy

NMR Study of Water Distribution inside Tomato Cells: Effects of Water Stress

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