Combined effect of arginine and fluoride on the growth of Lactobacillus rhamnosus GG

Abstract

The objectives of the in vitro study were: (1) to investigate the effect of combining L-arginine (Arg) and NaF on the growth of Lactobacillus rhamnosus GG (LRG); and (2) to identify an optimum synergistic concentration for the synbiotic (Arg + LRG)-fluoride (SF) therapy. 1% Arg + 2000-ppm NaF (A-SF) and 2% Arg + 2000-ppm NaF (B-SF) demonstrated antagonism against LRG (FIC > 4.0). Both XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) assays showed that A-SF and B-SF enhanced the growth of LRG when compared to 2000-ppm NaF and LRG control. Colony forming units, bacterial weight, and biofilm thickness of A-SF and B-SF were significantly higher than 2000-ppm NaF and LRG control. Biofilm imaging depicted that 2000-ppm NaF inhibited biofilm formation; while 1%/2% Arg, A-SF, and B-SF increased biofilm growth of LRG. Lactic acid formation was the lowest for 2000-ppm NaF, followed by A-SF and then B-SF. The SF buffer potential after 24 h was the highest for B-SF, and then A-SF. Biofilm pH for B-SF was closest to neutral. Fluoride, Arg and LRG bioavailability remained unaffected in B-SF. The relative gene expression for arcA, argG, and argH was significantly higher for B-SF than the respective controls. In conclusion, combining 2% Arg, 2000-ppm NaF, and LRG provides an optimum synbiotic-fluoride synergism.

Fluoride release potential of arginine-incorporated fluoride varnishes

“…The pH estimation was done using a combination double-junction gel filled electrode (Oakton-Cole Palmer, Thermo Fisher Scientific, Loughborough, UK) attached to a potentiometer (Eutech™ 2700 pH meter, Thermo Fisher Scientific) as per previous studies 25,30) . The pH electrode was calibrated against external pH standards -pH: 4.01, 7.0, and 10.01 on each occasion when pH was duly estimated.…”

“…As per previous studies 23,25,30) , the F analysis was performed using F-ion selective electrode (F-ISE; Orion™ Fluoride Electrodes, Thermo Fisher Scientific) attached to an ion benchtop meter (Eutech™ 2,700 pH meter, Thermo Fisher Scientific). For F-ISE calibration, the prepared external standards were subjected to mV potential determination with the ion meter.…”

Research Progress of Probiotic Therapy to Prevent Caries

“…Mohammed et al [36] found that L-arginine can promote the growth of Lactobacillus rhamnosus GG (LrG), and 2% L-arginine and LrG syngenics can jointly inhibit the growth of Streptococcus mutans, which has the potential to become antibacterial agents. The latest study by Mohammed et al [37] found that L-arginine (1% / 2%) combined with NaF Volume 4 Issue 6 2022 Issue 7 2022…”

Оценка антибактериальных эффектов триптофана и цинка аспартата как антибиопленочных агентов в комбинации с доксициклином in vitro

“…Недавние исследования показывают влияние комбинаций L-аргинина и фторида на рост Lactobacillus rhamnosus. Аргинин в зависимости от концентраций может увеличивать и задерживать пленкообразование, 2%-е содержание аминокислоты в среде обеспечивает оптимальный синбиотико-фторидный синергизм [16].…”