Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans

Abstract

Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydrolysis with high-pressure homogenization and ultrasonication were employed in this study to disrupt the cells of the microalga Neochloris oleoabundans. The cell disruption degree was investigated. The cell morphology before and after disruption was assessed with scanning and transmission electron microscopy. The energy requirements and the operation cost for wet cell disruption were also estimated. The highest disruption degree, up to 95.41%, assessed by accounting method was achieved by the combination of enzymatic hydrolysis and high-pressure homogenization. A lipid recovery of 92.6% was also obtained by the combined process. The combined process was found to be more efficient and economical compared with the individual process.

Effective production of bioenergy from marineChlorellasp. by high-pressure homogenization

“…[29] This result was confirmed by similar results from other studies. [30,31] Determination of the sugar content of the lipidextraction by-products…”

Microalgae Potential and Multiple Roles—Current Progress and Future Prospects—An Overview

“…The results revealed that A. brasilense boosted the growth of C. vulgaris through indole acetic acid (IAA) utilization of the amino acid tryptophan conversion process [38]. The Chlorella vulgaris exhibits poor harvesting due to flocculation efficiency of 0%-2% [39], but its co-culturing with bioflocculant-producing bacteria improved the harvesting. This highlights cost-effective production of microalgal biomass for biofuels.…”

Enzymatic pretreatment of Chlorella vulgaris for biogas production: Influence of urban wastewater as a sole nutrient source on macromolecular profile and biocatalyst efficiency

“…For instance, Passos and Ferrer (2015) demonstrated a break in the outer structure of the cell wall and damages in the intracellular structure after thermal pretreatment of Oocystis sp. while Wang et al (2015) showed a break down in the cell wall and leakage of intracellular material of Neochloris oleoabundans after combined mechanicalenzymatic pretreatment.…”