2021
DOI: 10.3390/ijms22147300
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Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells

Abstract: Protein dimerization plays a crucial role in the regulation of numerous biological processes. However, detecting protein dimers in a cellular environment is still a challenge. Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combination of fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis of single-color fluorescence fluctuation data. We named this analysis method brightness and diffusion global analysis (… Show more

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Cited by 4 publications
(3 citation statements)
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“…A similar type of PC diagram was obtained as a result of PCA analysis of the data for the FKBP12 protein (FK506 binding protein 12)-GFP [11] (Fig. 1b).…”
Section: Introductionsupporting
confidence: 59%
See 2 more Smart Citations
“…A similar type of PC diagram was obtained as a result of PCA analysis of the data for the FKBP12 protein (FK506 binding protein 12)-GFP [11] (Fig. 1b).…”
Section: Introductionsupporting
confidence: 59%
“…A number of methods, the most popular of which include PCH (photon counting histogram) [4], FIDA (fl uorescence intensity distribution analysis) [5], and their special modifi cations [6][7][8][9], have been proposed for analysis of the HNP [6][7][8][9]. Joint analysis of autocorrelation functions and HNP-BDGA (brightness and diff usion global analysis) -is used to improve the effi ciency of data analysis [10,11].…”
Section: Introductionmentioning
confidence: 99%
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