Combined metabolome and transcriptome analysis reveal the mechanism of selenate influence on the growth and quality of cabbage (Brassica oleracea var. capitata L.)

Yang, Xiaoyan; Liao, Xiaoli; Yu, Li; Rao, Shen; Chen, Qiangwen; Zhu, Zhenzhou; Cong, Xin; Zhang, Weiwei; Ye, Jiabao; Cheng, Shuiyuan; Xu, Feng

doi:10.1016/j.foodres.2022.111135

Cited by 27 publications

(13 citation statements)

References 55 publications

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“…Previous studies demonstrated that the transformation of selenocompounds varies with different plant species and different exogenous Se forms. The Allium and cruciferous vegetables mainly accumulated MeSeCys and SeMet, while most grains accumulated SeMet [ 58 ]. Funes-Collado et al [ 59 ] analyzed the changes in Se speciation of three leguminous sprouts (alfalfa, soy, and lentil) supplied with different Se-enriched solutions (mainly in selenite and selenate).…”

Section: Discussionmentioning

confidence: 99%

“…Determination of Se speciation in soybean seedlings was performed using liquid chromatography-HG-AFS (LC-AFS530, Beijing Haiguang Instrument, Beijing, China) following the method of Yang et al [ 58 ]. Then, 0.1 g of dry soybean tissue samples were enzymatically hydrolyzed with protease K and pronase E under ultrasonication at 37 °C for 1 h. The solution was then centrifuged at 10,000 rpm for 20 min.…”

Section: Methodsmentioning

confidence: 99%

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Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings

Xiong

Xiang

Xiao

et al. 2023

Plants

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Selenium (Se) is an essential element for mammals, and its deficiency in the diet is a global problem. Agronomic biofortification through exogenous Se provides a valuable strategy to enhance human Se intake. Selenium nanoparticles (SeNPs) have been regarded to be higher bioavailability and less toxicity in comparison with selenite and selenate. Still, little has been known about the mechanism of their metabolism in plants. Soybean (Glycine max L.) can enrich Se, providing an ideal carrier for Se biofortification. In this study, soybean sprouts were treated with SeNPs, and a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing was applied to clarify the underlying molecular mechanism of SeNPs metabolism. A total of 74,662 nonredundant transcripts were obtained, and 2109 transcription factors, 9687 alternative splice events, and 3309 long non-coding RNAs (lncRNAs) were predicted, respectively. KEGG enrichment analysis of the DEGs revealed that metabolic pathways, biosynthesis of secondary metabolites, and peroxisome were most enriched both in roots and leaves after exposure to SeNPs. A total of 117 transcripts were identified to be putatively involved in SeNPs transport and biotransformation in soybean. The top six hub genes and their closely coexpressed Se metabolism-related genes, such as adenylylsulfate reductase (APR3), methionine-tRNA ligase (SYM), and chloroplastic Nifs-like cysteine desulfurases (CNIF1), were screened by WGCNA and identified to play crucial roles in SeNPs accumulation and tolerance in soybean. Finally, a putative metabolism pathway of SeNPs in soybean was proposed. These findings have provided a theoretical foundation for future elucidation of the mechanism of SeNPs metabolism in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings

Xiong

Xiang

Xiao

et al. 2023

Plants

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“…At present, most studies focus on the effect of exogenous selenium on the content of secondary metabolites in tea, but the molecular mechanism is not clear. At present, only a few pieces of literature have reported that the intermediate products of the selenium metabolic pathway in plants can provide precursors for the synthesis of important secondary metabolites such as flavonoids and phenolic acids [ 52 ]. The mechanism of selenium affecting the accumulation of secondary metabolites is also the focus of future research.…”

Section: Effects Of Selenium On Tea Plant Qualitymentioning

confidence: 99%

Research Progress on the Effects of Selenium on the Growth and Quality of Tea Plants

Xiang

Rao

Chen

et al. 2022

Plants

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Selenium (Se) is an essential trace element for humans and animals, and it plays an important role in immune regulation and disease prevention. Tea is one of the top three beverages in the world, and it contains active ingredients such as polyphenols, theanine, flavonoids, and volatile substances, which have important health benefits. The tea tree has suitable Se aggregation ability, which can absorb inorganic Se and transform it into safe and effective organic Se through absorption by the human body, thereby improving human immunity and preventing the occurrence of many diseases. Recent studies have proven that 50~100.0 mg/L exogenous Se can promote photosynthesis and absorption of mineral elements in tea trees and increase their biomass. The content of total Se and organic selenides in tea leaves significantly increases and promotes the accumulation of polyphenols, theanine, flavonoids, and volatile secondary metabolites, thereby improving the nutritional quality of tea leaves. This paper summarizes previous research on the effects of exogenous Se treatment on the growth and quality of tea trees to provide a theoretical basis and technical support for the germplasm selection and exploitation of Se-rich tea.

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“…2 ) [ 93 – 95 ]. The edible parts of crop plants like leaves, stems, roots, seeds, fruits, and flowers are the main source of dietary Se for humans and animals [ 96 ]. After Se uptake from soil, the selenate and selenite species are mainly transformed to selenocysteine (SeCys), selenomethionine (SeMet), and methylselenocystein (MeSeCys), which then can participate in protein synthesis.…”

Section: Selenium Improves Crop Quality and Resistance To Environment...mentioning

confidence: 99%

Selenium species transforming along soil–plant continuum and their beneficial roles for horticultural crops

Guo

Zeng

et al. 2022

Horticulture Research

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Selenium (Se) acquirement from daily diet can help reduce risk of many diseases. The edible parts of crop plants are the main source of dietary Se, while the Se content in crops is determined by Se bioavailability in soil. We summarize recent research on the biogeochemical cycle of Se driven by specific microorganisms and emphasize the oxidizing process in the Se cycle. Moreover, we discuss how plant root exudates and rhizosphere microorganisms affect soil Se availability. Finally, we cover beneficial microorganisms, including endophytes, that promote crop quality and improve crop tolerance to environmental stresses. Se availability to plants depends on the balance between adsorption and desorption, reduction, methylation and oxidation, which are determined by interactions among soil properties, microbial communities and plants. Reduction and methylation processes governed by bacteria or fungi lead to declined Se availability, while Se oxidation regulated by Se-oxidizing microorganisms increases Se availability to plants. Despite a much lower rate of Se oxidization compared to reduction and methylation, the potential roles of microbial communities in increasing Se bioavailability are probably largely underestimated. Enhancing Se oxidation and Se desorption are crucial for the promotion of Se bioavailability and uptake, particularly in Se-deficient soils. Beneficial roles of Se are reported in terms of improved crop growth and quality, and enhanced protection against fungal diseases and abiotic stress through improved photosynthetic traits, increased sugar and amino acid contents, and promoted defense systems. Understanding Se transformation along the plant-soil continuum is crucial for agricultural production and even for human health.

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Combined metabolome and transcriptome analysis reveal the mechanism of selenate influence on the growth and quality of cabbage (Brassica oleracea var. capitata L.)

Cited by 27 publications

References 55 publications

Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings

Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings

Research Progress on the Effects of Selenium on the Growth and Quality of Tea Plants

Selenium species transforming along soil–plant continuum and their beneficial roles for horticultural crops

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