Combined metabolomic and transcriptomic analysis reveals the characteristics of the lignan in Isatis indigotica Fortune

Su, Yong; Huang, Jiabin; Guo, Qiaosheng; Shi, Hongzhuan; Wei, Min; Wang, Chengxiang; Zhao, Kun; Bao, Tao

doi:10.1016/j.gene.2023.147752

Cited by 1 publication

(1 citation statement)

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“…In recent years, transcriptomics and metabolomics have been intensively utilized to screen lignan biosynthesis-related metabolites and genes [ 19 – 21 ]. Although several structural genes and transcription factors implicated in lignan biosynthesis and regulation have been identified in many plants, the regulation of species-specific lignan biosynthesis pathways has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis of the metabolome and transcriptome provides insights into the mechanisms of lignan biosynthesis in Herpetospermum pedunculosum (Cucurbitaceae)

Zhu,

Chen,

Sun

et al. 2024

BMC Genomics

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Background Herpetospermum pedunculosum (Ser.) C. B. Clarke is a traditional Chinese herbal medicine that heavily relies on the lignans found in its dried ripe seeds (Herpetospermum caudigerum), which have antioxidant and hepatoprotective functions. However, little is known regarding the lignan biosynthesis in H. pedunculosum. In this study, we used metabolomic (non-targeted UHPLC-MS/MS) and transcriptome (RNA-Seq) analyses to identify key metabolites and genes (both structural and regulatory) associated with lignan production during the green mature (GM) and yellow mature (YM) stages of H. pedunculosum. Results The contents of 26 lignan-related metabolites and the expression of 30 genes involved in the lignan pathway differed considerably between the GM and YM stages; most of them were more highly expressed in YM than in GM. UPLC-Q-TOF/MS confirmed that three Herpetospermum-specific lignans (including herpetrione, herpetotriol, and herpetin) were found in YM, but were not detected in GM. In addition, we proposed a lignan biosynthesis pathway for H. pedunculosum based on the fundamental principles of chemistry and biosynthesis. An integrated study of the transcriptome and metabolome identified several transcription factors, including HpGAF1, HpHSFB3, and HpWOX1, that were highly correlated with the metabolism of lignan compounds during seed ripening. Furthermore, functional validation assays revealed that the enzyme 4-Coumarate: CoA ligase (4CL) catalyzes the synthesis of hydroxycinnamate CoA esters. Conclusion These results will deepen our understanding of seed lignan biosynthesis and establish a theoretical basis for molecular breeding of H. pedunculosum.

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Section: Introductionmentioning

confidence: 99%