Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology

Abstract

To meet the needs for polymer particulates for the flooding of oil fields, a composite crosslinked copolymer of hydrophilic monomers [acrylamide (AM) and acrylic acid (AA)] and a hydrophobic monomer [methyl methacrylate (MMA)] was synthesized. In this study, we first added a rare‐earth thermal stabilizer and inorganic montmorillonite (MMT) clay to the copolymer; this showed that the composite crosslinked copolymer exhibited good water‐swelling performance at 200°C, with gel static grade of 5, a toughness index of 1.1170, and a salinity limit of 250,000 mg/L. It also had better injection abilities. The AM/AA/MMA ratio was 4 : 1 : 0.03 w/w, the crosslinker additive content was 0.03 wt %, the thermal stabilizer additive content was 5 wt %, and the clay (MMT) additive content was 13 wt %. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 4429–4433, 2006

The use of Urovysion™ fluorescence in situ hybridization in the diagnosis and surveillance of non-urothelial carcinoma of the bladder

“…9,25 Urovysiont FISH is one such molecular test that has been shown to have high sensitivity and specificity for the detection of urothelial carcinoma in urine. 8,14,19,21,26 We recently observed several cases of non-urothelial carcinoma that were Urovysiont FISH-positive. Others have also observed these coincidental overlaps with non-urothelial tumors.…”

“…17,18 Urovysiont FISH has a reported sensitivity of 73-92%, a specificity of 89-96% for urothelial carcinoma detection and several studies have confirmed its usefulness in the diagnosis and surveillance of these tumors. 8,14,[19][20][21][22] The test is designed to detect aneuploidy for chromosomes 3, 7, 17, and loss of the 9p21 locus in malignant urothelial cells that are shed in the urine of persons with urothelial carcinoma. These represent some of the most common chromosomal abnormalities in urothelial carcinoma.…”

Multiprobe Fluorescence in situ Hybridization (UroVysion) for the Detection of Urothelial Carcinoma – FISHing for the Right Catch

“…They generate image galleries that need to be edited at the computer screen for the final result. There are currently 2 US Food and Drug Administration-approved systems for automated UroVysion analysis including the Duet System (Bioview Ltd., Rehovoth, Israel, and Billarica, Mass., USA) [27,28] and the Ikoniscope (Ikonisys Inc., New Haven, Conn., USA) [29]. These systems allow for permanent documentation and retrospective re-analysis of the FISH results.…”

Using urine microscopy and cytology for early detection of bladder cancer in male patients with lower urinary tract symptoms

“…Based on the seven questions, the symptoms were divided into mild (0-7), moderate (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), and severe (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). Patient with significant irritative symptoms was defined as the total scores in irritative domains (frequency, urgency, and nocturia) of nine or more.…”