Combined Non-Steroidal Therapy in Experimental Corneal Injury

Abstract

Purpose: The effects of anti-inflammatory non-steroidal therapy combined with free-radical scavengers were studied and compared to corticosteroid use in the treatment of experimental corneal injury. Method: Eighty New Zealand albino rabbits were used in this study. A corneal alkali burn was induced by applying 1-N NaOH filter paper on the central axis of the right cornea for 30 s. Animals were distributed into five treatment groups: group 1 (control group) was only given gentamicin; group 2 was treated with 0.5% dimethylthiourea (DMU); group 3 received 1 % dexamethasone; group 4 was given combined 0.5% DMU and 1% indomethacin; group 5 was treated with 0.5% DMU and 0.1% diclofenac sodium. One 50-μl drop of gentamicin was instilled every 12 h, whereas the other drugs were instilled every 6 h (50 μl). All groups received the same antibiotic treatment as the control group. The animals were killed on the 5th day. Inflammatory index, area and perimeter of the wounded corneal zone, and corneal transparency were evaluated. Results: No significant differences in the inflammatory index were found between the treatment groups and the control group after 72 h. Significant differences (p < 0.001) were observed at 24 h in groups 3–5 when compared with the control group. Planimetry showed significant differences in group 4 when compared with the other groups (p < 0.05). Corneal transparency study showed statistically significantly better values in groups 4 and 5, when compared with the other groups, including group 3 (p < 0.05). Conclusions: The use of 0.5% DMU combined with 1% indomethacin can be considered an alternative to corticosteroid treatment in our experimental chemical corneal injury.

Inhibition of Corneal Neovascularization by Hydrazinocurcumin

“…It is reported that corneal neovascularization induced through two intrastromal implantations of VEGF polymer is associated with corneal inflammation and resembles inflammatory corneal diseases [20,21]. Therefore, corneal neovascularisation induced through two intrastromal implantations of VEGF polymer serves as a suitable animal model for the study of inflammatory corneal neovascularization.…”

Clinical and Experimental Research of Corneal Lymphangiogenesis after Keratoplasty

“…Serial photographs of the human corneas were taken before keratoplasty. The IF was analyzed as previously described [12]. Briefly, the IF was analyzed based on the following parameters: ciliary hyperemia (0 = absent; 1 = present but less than 1 mm; 2 = present between 1 and 2 mm; 3 = present and more than 2 mm); central corneal edema (0 = absent; 1 = present with visible iris details; 2 = present without visible iris details; 3 = present without visible pupil); peripheral corneal edema (0 = absent; 1 = present with visible iris details; 2 = present without visible iris details; 3 = present with no visible iris).…”

A Mouse Model of Limbal Stem Cell Deficiency Induced by Topical Medication With the Preservative Benzalkonium Chloride

“…21 The inflammatory index was analyzed based on the following parameters: ciliary hyperemia (absent, 0; present but less than 1 mm, 1; present between 1 and 2 mm, 2; present and more than 2 mm, 3), central corneal edema (absent, 0; present with visible iris details, 1; present without visible iris details, 2; present …”