Combined –omics framework reveals how ant symbionts benefit the Neotropical ant-plant Tococa quadrialata at different levels

Müller, Andrea T.; Reichelt, Michael; Cosio, Eric G.; Salinas, Norma; Nina, Alex; Wang, Ding; Moossen, Heiko; Geilmann, Heike; Gershenzon, Jonathan; Köllner, Tobias G.; Mithöfer, Axel

doi:10.1016/j.isci.2022.105261

Cited by 9 publications

(3 citation statements)

References 109 publications

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“…The extract was diluted 1:10 (v/v) with water containing the 13 C, 15 N-labeled amino acid mix (Isotec, Miamisburg, OH, USA) as the internal standard. The amino acids in the diluted extracts were directly analyzed using LC-MS/MS as described in [39] using a QTRAP 6500 mass spectrometer (Sciex, Darmstadt, Germany) coupled to an Agilent 1260 series HPLC system.…”

Section: Extraction and Quantification Of Amino Acids Using Lc-ms/msmentioning

confidence: 99%

“…The extraction procedure and phytohormone determination was carried out according to Müller et al (2022) [39]. The tissue was extracted and homogenized in 1.5 mL methanol containing 60 ng D4-SA (Santa Cruz Biotechnology, Santa Cruz, CA, USA), 60 ng D6-JA (HPC Standards GmbH, Borsdorf, Germany), 60 ng D6-ABA (Toronto Research Chemicals, Toronto, ON, Canada) and 12 ng D6-JA-Ile (HPC Standards GmbH, Borsdorf, Germany) as the internal standards.…”

Section: Extraction and Quantification Of Phytohormones Using Lc-ms/msmentioning

confidence: 99%

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The Beneficial Fungus Mortierella hyalina Modulates Amino Acid Homeostasis in Arabidopsis under Nitrogen Starvation

Svietlova,

Reichelt,

Zhyr

et al. 2023

IJMS

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Non-mycorrhizal but beneficial fungi often mitigate (a)biotic stress-related traits in host plants. The underlying molecular mechanisms are mostly still unknown, as in the interaction between the endophytic growth-promoting soil fungus Mortierella hyalina and Arabidopsis thaliana. Here, abiotic stress in the form of nitrogen (N) deficiency was used to investigate the effects of the fungus on colonized plants. In particular, the hypothesis was investigated that fungal infection could influence N deficiency via an interaction with the high-affinity nitrate transporter NRT2.4, which is induced by N deficiency. For this purpose, Arabidopsis wild-type nrt2.4 knock-out and NRT2.4 reporter lines were grown on media with different nitrate concentrations with or without M. hyalina colonization. We used chemical analysis methods to determine the amino acids and phytohormones. Experimental evidence suggests that the fungus does not modulate NRT2.4 expression under N starvation. Instead, M. hyalina alleviates N starvation in other ways: The fungus supplies nitrogen (15N) to the N-starved plant. The presence of the fungus restores the plants’ amino acid homeostasis, which was out of balance due to N deficiency, and causes a strong accumulation of branched-chain amino acids. We conclude that the plant does not need to invest in defense and resources for growth are maintained, which in turn benefits the fungus, suggesting that this interaction should be considered a mutualistic symbiosis.

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Section: Extraction and Quantification Of Amino Acids Using Lc-ms/msmentioning

confidence: 99%

Section: Extraction and Quantification Of Phytohormones Using Lc-ms/msmentioning

confidence: 99%

The Beneficial Fungus Mortierella hyalina Modulates Amino Acid Homeostasis in Arabidopsis under Nitrogen Starvation

Svietlova,

Reichelt,

Zhyr

et al. 2023

IJMS

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“…However, despite ample ecological study (Mayer et al, 2014), few studies have applied ‘omics approaches to ant-plant interactions. On the plant side, metabolomic and transcriptomic comparisons have assessed the effects of ant colonization on Tococa ant-plants (Müller et al, 2022). On the ant side, Malé et al (2017) suggested that the cGMP-PKG signaling pathway modulates ant protection of plants, while Rubin and Moreau (2016) found higher rates of molecular evolution genome-wide in mutualistic acacia-ants compared to their non-mutualistic close relatives, but there has been little additional research.…”

Section: Introductionmentioning

confidence: 99%

In sickness and in health: RNA-Seq finds viruses associated with mutualist quality in the Amazonian plant-antAllomerus octoarticulatus

Tocora,

Reid,

Huisken

et al. 2024

Preprint

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Ant-plant symbioses are classic examples of mutualism in which ant "bodyguards" defend myrmecophytic plants against enemies in exchange for nest sites and often food. We used RNA-Seq to profile the transcriptomes ofAllomerus octoarticulatusant workers, which aggressively defend the Amazonian plantCordia nodosaagainst herbivores, but to varying degrees. Field behavioral assays with herbivores in the Peruvian Amazon showed striking variation among colonies in the relative zeal with whichA. octoarticulatusworkers defend their host plant. Highly effective and ineffective bodyguards differed in their gene expression profiles, which revealed viral infections significantly associated with ant bodyguarding behavior. Transcripts from eight new positive-sense single-stranded RNA viruses were differentially expressed between colonies with high- or low-quality bodyguards. Colonies of high- and low-quality bodyguards were infected by distinct viruses, including viruses clustering phylogenetically with viruses known to cause aggression or reduced locomotion, respectively, in bees. Gene expression, including of immunity-related genes, also differed between broodcare workers and bodyguard ants, suggesting bodyguarding is a distinct worker task. Ant colony health and viral infections may influence ant cooperation with plants in ant-plant mutualisms.

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Fusarium incarnatum Strain K23 Reprograms the Response of Tomato Seedlings to Salt Stress

Pallavi,

Reddy,

Govind

et al. 2024

J Plant Growth Regul

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Combined –omics framework reveals how ant symbionts benefit the Neotropical ant-plant Tococa quadrialata at different levels

Cited by 9 publications

References 109 publications

The Beneficial Fungus Mortierella hyalina Modulates Amino Acid Homeostasis in Arabidopsis under Nitrogen Starvation

The Beneficial Fungus Mortierella hyalina Modulates Amino Acid Homeostasis in Arabidopsis under Nitrogen Starvation

In sickness and in health: RNA-Seq finds viruses associated with mutualist quality in the Amazonian plant-antAllomerus octoarticulatus

Fusarium incarnatum Strain K23 Reprograms the Response of Tomato Seedlings to Salt Stress

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