Combined resveratrol and vitamin D treatment ameliorate inflammation-related liver fibrosis, ER stress, and apoptosis in a high-fructose diet/streptozotocin-induced T2DM model

Anapali, Merve; Dağıstanlı, Fatma Kaya; Akdemir, Ayse Seda; Aydemir, Duygu; Ulusu, Nuriye Nuray; Ulutin, Turgut; Uysal, Ömer; Tanrıverdi, Gamze; Öztürk, Melek

doi:10.1007/s00418-022-02131-y

Cited by 10 publications

(5 citation statements)

References 83 publications

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“…In this study, we found increased expression of CHOP and GRP78 in PA-induced MIN6 cells compared with normal cells, which was reversed after 1,25(OH) 2 D 3 treatment. A recent study has demonstrated that vitamin D combined with resveratrol can inhibit ERS to alleviate the symptoms of T2DM 58 . Notably, we found that the OS inhibitor, NAC, enhanced the alleviating effects of vitamin D on cell death, GSIS-insulin deficiency, inflammation, OS, and ERS in PA-induced MIN6 cells.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment

Liu,

Zhang,

Shi

et al. 2023

Exp Clin Endocrinol Diabetes

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Objective Type 2 diabetes mellitus (T2DM) is a common metabolic disorder with rising incidence worldwide. This study explored the anti-T2DM role of vitamin D, thereby providing novel therapeutic strategies. Methods C57BL/6 J mice and MIN6 cells were used to induce in vivo T2DM and damaged β-cell models, respectively. Body weights, fasting blood glucose, and fasting insulin were measured in mice. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were conducted on mice. Lipid indices (TG, TC, LDL-C, and HDL-C) were detected in mouse serum. Hematoxylin-eosin staining was used to evaluate pancreatic tissue injury. ELISA was used to assess insulin and oxidative stress (OS) markers (MDA, GSH, and SOD) in mice and MIN6 cells. Production of ROS was detected in islet β-cells and MIN6 cells. Cell viability and apoptosis were evaluated using CCK-8 and flow cytometry, respectively. QRT-PCR and western blotting were used to detect pro-inflammatory factors (TNF-α and IL-6) and endoplasmic reticulum stress (ERS) markers (CHOP and GRP78), respectively. Results Vitamin D reduced body weights, fasting blood glucose, and insulin and ameliorated glucose tolerance and insulin sensitivity in T2DM mice. Besides, vitamin D decreased serum TG, TC, LDL-C, and increased HDL-C in T2DM mice. Vitamin D inhibited pancreatic histopathological injury, cell apoptosis, OS, and β-cell decline in T2DM mice. Moreover, vitamin D alleviated cell death, insufficient insulin secretion, inflammation, OS, and ERS in damaged MIN6 cells. Notably, N-acetyl-L-cysteine (an OS inhibitor) enhanced these effects of vitamin D. Conclusions Vitamin D relieved T2DM symptoms by alleviating OS-induced β-cell impairment.

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Section: Discussionmentioning

confidence: 99%

Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment

Liu,

Zhang,

Shi

et al. 2023

Exp Clin Endocrinol Diabetes

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“…Depending on the organ to be studied, the demonstration of glucose transports such as insulin receptors, insulin receptor substrates, Glut-1, -2, -3, and -4 by molecular or biochemical techniques constitute the most basic stages of the study. 90 , 91 In these studies, obesity develops due to an HFD, unlike the type 1 diabetes model ( Table 7 ). 92 - 100 …”

Section: In Vitro Enzyme Activitymentioning

confidence: 99%

Experimental Approaches to Diabetes Mellitus

Yayla¹,

Binnetoğlu²

2023

Eurasian J Med

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One of the most common health problems today, diabetes is a serious, chronic, and complex disease characterized by high blood glucose levels. Nowadays, experimental diabetes models are being developed to study existing diabetes in depth, to improve diabetes medications, or to develop new medications. The protocols developed to date to create an experimental diabetes model are finalized in different time intervals and depending on various factors. With these models, which can be designed in vivo and in vitro, a picture similar to type 1 and type 2 diabetes can be created. In this review, we aimed to present the methodology, advantages, and disadvantages of all currently used experimental diabetes models in the light of current literature.

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“…Additionally, resveratrol ameliorates high glucose-induced endoplasmic reticulum stress, cellular apoptosis and hyperuricemia-induced renal injury ( Xiao et al, 2021 ; Zhang et al, 2020 ). Moreover, the anti-fibrotic effects of resveratrol have been well documented in several organ systems, including kidney ( Wang et al, 2022b ; Zhang et al, 2019 ), liver ( Anapali et al, 2022 ; Mostafa et al, 2023 ), lung ( Liu et al, 2023 ; Singh et al, 2023 ) and prostate ( Zeng et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol inhibits calcium oxalate crystal growth, reduces adhesion to renal cells and induces crystal internalization into the cells, but promotes crystal aggregation

Peerapen,

Putpeerawit,

Boonmark

et al. 2024

Current Research in Food Science

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Combined resveratrol and vitamin D treatment ameliorate inflammation-related liver fibrosis, ER stress, and apoptosis in a high-fructose diet/streptozotocin-induced T2DM model

Cited by 10 publications

References 83 publications

Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment

Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment

Experimental Approaches to Diabetes Mellitus

Resveratrol inhibits calcium oxalate crystal growth, reduces adhesion to renal cells and induces crystal internalization into the cells, but promotes crystal aggregation

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