Combined transcriptomic and proteomic analysis of developmental features in the immune system of Plutella xylostella during larva-to-adult metamorphosis

Zhang, Zhantao; Zhu, Shaojie; Mandal, Surajit De; Gao, Yanfu; Yu, Jing; Zeng, Lu; Huang, Junhai; Zafar, Junaid; Jin, Fengliang; Xu, Xi

doi:10.1016/j.ygeno.2022.110381

Cited by 11 publications

(10 citation statements)

References 62 publications

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“…Since then, peaks of AMP expression at metamorphosis have been reported in many insects, either systemically or locally in the gut. For example, AMPs were found transcriptionally upregulated with other immune effectors in the pupae of several other lepidopteran species [72][73][74][75][76]82] and red flour beetle T . castaneum [77], in the absence of experimental infections (table 1).…”

Section: Amps' Involvement During Developmental Processesmentioning

confidence: 99%

Cereal weevils' antimicrobial peptides: at the crosstalk between development, endosymbiosis and immune response

Galambos,

Vincent-Monegat,

Vallier

et al. 2024

Phil. Trans. R. Soc. B

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Interactions between animals and microbes are ubiquitous in nature and strongly impact animal physiology. These interactions are shaped by the host immune system, which responds to infections and contributes to tailor the associations with beneficial microorganisms. In many insects, beneficial symbiotic associations not only include gut commensals, but also intracellular bacteria, or endosymbionts. Endosymbionts are housed within specialized host cells, the bacteriocytes, and are transmitted vertically across host generations. Host–endosymbiont co-evolution shapes the endosymbiont genome and host immune system, which not only fights against microbial intruders, but also ensures the preservation of endosymbionts and the control of their load and location. The cereal weevil Sitophilus spp. is a remarkable model in which to study the evolutionary adaptation of the immune system to endosymbiosis owing to its binary association with a unique, relatively recently acquired nutritional endosymbiont, Sodalis pierantonius . This Gram-negative bacterium has not experienced the genome size shrinkage observed in long-term endosymbioses and has retained immunogenicity. We focus here on the sixteen antimicrobial peptides (AMPs) identified in the Sitophilus oryzae genome and their expression patterns in different tissues, along host development or upon immune challenges, to address their potential functions in the defensive response and endosymbiosis homeostasis along the insect life cycle. This article is part of the theme issue ‘Sculpting the microbiome: how host factors determine and respond to microbial colonization’.

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Section: Amps' Involvement During Developmental Processesmentioning

confidence: 99%

Cereal weevils' antimicrobial peptides: at the crosstalk between development, endosymbiosis and immune response

Galambos,

Vincent-Monegat,

Vallier

et al. 2024

Phil. Trans. R. Soc. B

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“…The purified proteins were washed out using the elution buffer (20 mM Tris-HCl, 500 mM NaCl, pH 7.4, 500mM imidazole). The buffer was then replaced with PBS through dialysis, and the purified protein was ultimately stored in PBS buffer at 4 • C [40].…”

Section: The Prokaryotic Expression and Purification Of Recombinant P...mentioning

confidence: 99%

“…Additionally, significant knowledge gaps in the regulatory mechanisms of miRNA-based pest control pose further challenges for its field application. In our previous study, three PAPs (PAP1, PAP3, and PAP3a) were identified in P. xylostella [40], and based on transcriptome data, we predicted that non-coding RNAs might regulate them. Here, we aim to delineate the interaction mechanism between LncRNA and miRNA of P. xylostella infected with Metarhizium anisopliae, using in vitro and in vivo experiments.…”

Section: Introductionmentioning

confidence: 96%

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

Zhang,

Jin,

Huang

et al. 2024

IJMS

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MicroRNAs (miRNAs) play a pivotal role in important biological processes by regulating post-transcriptional gene expression and exhibit differential expression patterns during development, immune responses, and stress challenges. The diamondback moth causes significant economic damage to crops worldwide. Despite substantial advancements in understanding the molecular biology of this pest, our knowledge regarding the role of miRNAs in regulating key immunity-related genes remains limited. In this study, we leveraged whole transcriptome resequencing data from Plutella xylostella infected with Metarhizium anisopliae to identify specific miRNAs targeting the prophenoloxidase-activating protease1 (PAP1) gene and regulate phenoloxidase (PO) cascade during melanization. Seven miRNAs (pxy-miR-375-5p, pxy-miR-4448-3p, pxy-miR-279a-3p, pxy-miR-3286-3p, pxy-miR-965-5p, pxy-miR-8799-3p, and pxy-miR-14b-5p) were screened. Luciferase reporter assays confirmed that pxy-miR-279a-3p binds to the open reading frame (ORF) and pxy-miR-965-5p to the 3′ untranslated region (3′ UTR) of PAP1. Our experiments demonstrated that a pxy-miR-965-5p mimic significantly reduced PAP1 expression in P. xylostella larvae, suppressed PO activity, and increased larval mortality rate. Conversely, the injection of pxy-miR-965-5p inhibitor could increase PAP1 expression and PO activity while decreasing larval mortality rate. Furthermore, we identified four LncRNAs (MSTRG.32910.1, MSTRG.7100.1, MSTRG.6802.1, and MSTRG.22113.1) that potentially interact with pxy-miR-965-5p. Interference assays using antisense oligonucleotides (ASOs) revealed that silencing MSTRG.7100.1 and MSTRG.22113.1 increased the expression of pxy-miR-965-5p. These findings shed light on the potential role of pxy-miR-965-5p in the immune response of P. xylostella to M. anisopliae infection and provide a theoretical basis for biological control strategies targeting the immune system of this pest.

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“…Melanin is toxic to parasites, bacteria, fungi, and viruses [ 106 ]. It was reported that the expression of Serpin and PAP s was significantly affected by Bt HD73 in P. xylostella [ 112 ]. Serine proteases and serine protease inhibitors are also highly regulated in Bt -susceptible and -resistant Anticarsia gemmatalis strains in response to Bt [ 113 ].…”

Section: Immunity Induced By Btmentioning

confidence: 99%

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Xiao

Yao

Bai

et al. 2023

Insects

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Bacillus thuringiensis (Bt) is the safest, economically successful entomopathogen to date. It is extensively produced in transgenic crops or used in spray formulations to control Lepidopteran pests. The most serious threat to the sustainable usage of Bt is insect resistance. The resistance mechanisms to Bt toxins depend not only on alterations in insect receptors, but also on the enhancement of insect immune responses. In this work, we review the current knowledge of the immune response and resistance of insects to Bt formulations and Bt proteins, mainly in Lepidopteran pests. We discuss the pattern recognition proteins for recognizing Bt, antimicrobial peptides (AMPs) and their synthetic signaling pathways, the prophenoloxidase system, reactive oxygen species (ROS) generation, nodulation, encapsulation, phagocytosis, and cell-free aggregates, which are involved in immune response reactions or resistance to Bt. This review also analyzes immune priming, which contributes to the evolution of insect resistance to Bt, and puts forward strategies to improve the insecticidal activity of Bt formulations and manage insect resistance, targeting the insect immune responses and resistance.

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Combined transcriptomic and proteomic analysis of developmental features in the immune system of Plutella xylostella during larva-to-adult metamorphosis

Cited by 11 publications

References 62 publications

Cereal weevils' antimicrobial peptides: at the crosstalk between development, endosymbiosis and immune response

Cereal weevils' antimicrobial peptides: at the crosstalk between development, endosymbiosis and immune response

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

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