Combined transcriptomic, proteomic and genomic analysis identifies reproductive-related proteins and potential modulators of female behaviors in Spodoptera litura

Xiao, Haiyan; Li, Gen-Ceng; Wang, Zhengquan; Guo, Yu-Ruo; Liu, Nai-Yong

doi:10.1016/j.ygeno.2021.04.006

Cited by 18 publications

(23 citation statements)

References 83 publications

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“…Combining FPKM and RT-PCR approaches, the expression profiles of 23 DabiCSPs were determined. In line with those from other moths (Gong et al, 2007;Xiao et al, 2021), DabiCSPs were broadly expressed in various tissues, and none of the tissue-specific genes were found (Figure 3B). In particular, some of them displayed a very high transcription in each tissue (FPKM > 180), such as DabiCSP5, CSP9, and CSP13.…”

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Chemosensory Proteinssupporting

confidence: 82%

“…Intriguingly, 5 DabiOBPs (DabiOBP9, OBP18, OBP25, OBP36, and OBP37) had obviously high expression in male abdomens (FPKM > 13) but very low levels in females (FPKM < 1) (Supplementary Table 4). As indicated in S. litura, a number of SlitOBPs were abundantly presented in male reproductive tissues (Xiao et al, 2021). Thus, the 5 DabiOBPs may be candidate reproductive-related genes for modulating female or male reproductive-associated behaviors.…”

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Odorant Binding Proteinsmentioning

confidence: 80%

“…However, two subfamily expansions were observed in some species, including 9 BmorOBPs in Minus-C OBPs (each five in D. abietella and S. litura) and 17 SlitOBPs in ABP Is (8 in B. mori and 9 in D. abietella). In addition, the ortholog of PBP4, which was previously defined as a novel subclass of the Lepidopteraspecific PBP/GOBPs (Xiao et al, 2021), was not found in D. abietella (Figure 2A).…”

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Odorant Binding Proteinsmentioning

confidence: 84%

“…In the trees of OBPs and CSPs, the proteins from B. mori, D. abietella and S. litura were selected (Gong et al, 2009;Xiao et al, 2021). The OR tree was inferred with the ORs from D. abietella, Helicoverpa armigera, and O. furnacalis (Zhang T. et al, 2015;Guo et al, 2020).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

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Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Wang

Chun

et al. 2021

Front. Ecol. Evol.

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In Lepidoptera, RNA sequencing has become a useful tool in identifying chemosensory genes from antennal transcriptomes, but little attention is paid to non-antennal tissues. Though the antennae are primarily responsible for olfaction, studies have found that a certain number of chemosensory genes are exclusively or highly expressed in the non-antennal tissues, such as proboscises, legs and abdomens. In this study, we report a global transcriptome of 16 tissues from Dioryctria abietella, including chemosensory and non-chemosensory tissues. Through Illumina sequencing, totally 952,658,466 clean reads were generated, summing to 142.90 gigabases of data. Based on the transcriptome, 235 chemosensory-related genes were identified, comprising 42 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs), 75 odorant receptors (ORs), 62 gustatory receptors (GRs), 30 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). Compared to a previous study in this species, 140 novel genes were found. A transcriptome-wide analysis combined with PCR results revealed that except for GRs, the majority of other five chemosensory gene families in Lepidoptera were expressed in the antennae, including 160 chemosensory genes in D. abietella. Using phylogenetic and expression profiling analyses, members of the six chemosensory gene repertoires were characterized, in which 11 DabiORs were candidates for detecting female sex pheromones in D. abietella, and DabiOR23 may be involved in the sensing of plant-derived phenylacetaldehyde. Intriguingly, more than half of the genes were detected in the proboscises, and one fourth of the genes were found to have the expression in the legs. Our study not only greatly extends and improves the description of chemosensory genes in D. abietella, but also identifies potential molecular targets involved in olfaction, gustation and non-chemosensory functions for control of this pest.

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Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Chemosensory Proteinssupporting

confidence: 82%

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Odorant Binding Proteinsmentioning

confidence: 80%

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Odorant Binding Proteinsmentioning

confidence: 84%

Section: Phylogenetic Analysismentioning

confidence: 99%

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Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Wang

Chun

et al. 2021

Front. Ecol. Evol.

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“…Unfortunately, B. odoriphaga has developed increased resistance to insecticides because of heavy reliance on chemical insecticides ( Chen et al, 2017 ). To investigate insecticide resistance mechanisms and promote integrated pest management (IPM) strategies, researchers have studied several pests over the past few decades and achieved important progress in several areas, including genomics ( Xiao et al, 2021 ), transcriptomics ( Cheng et al, 2020 ; Nazar et al, 2020 ; Nor Muhammad et al, 2020 ; Wang et al, 2020 ; Fu et al, 2021 ; Zou et al, 2021 ), proteomics ( Prajapati et al, 2020 ; Chen et al, 2021 ), insecticide resistance ( Wang et al, 2020 ; Gong et al, 2021 ; Ullah et al, 2021 ), RNA interference ( Koo et al, 2020 ; Silver et al, 2021 ), and gene functions ( Yu et al, 2020 ; Li L. L. et al, 2021 ; Luo et al, 2021 ). However, further studies on the mechanism of insecticide resistance are required to clarify the genes directly involved in resistance and regulatory mechanisms associated with those genes.…”

Section: Introductionmentioning

confidence: 99%

Selection and Validation of Reference Genes for RT-qPCR Normalization in Bradysia odoriphaga (Diptera: Sciaridae) Under Insecticides Stress

Huang

Chen

et al. 2022

Front. Physiol.

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Bradysia odoriphaga (Diptera: Sciaridae) is the most serious root maggot pest which causes substantial damage to the Chinese chive. Organophosphate (OP) and neonicotinoid insecticides are widely used chemical pesticides and play important roles in controlling B. odoriphaga. However, a strong selection pressure following repeated pesticide applications has led to the development of resistant populations of this insect. To understand the insecticide resistance mechanism in B. odoriphaga, gene expression analysis might be required. Appropriate reference gene selection is a critical prerequisite for gene expression studies, as the expression stability of reference genes can be affected by experimental conditions, resulting in biased or erroneous results. The present study shows the expression profile of nine commonly used reference genes [elongation factor 1α (EF-1α), actin2 (ACT), elongation factor 2α (EF-2α), glucose-6-phosphate dehydrogenase (G6PDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein L10 (RPL10), ribosomal protein S3 (RPS3), ubiquitin-conjugating enzyme (UBC), and α-tubulin (TUB)] was systematically analyzed under insecticide stress. Moreover, we also evaluated their expression stability in other experimental conditions, including developmental stages, sexes, and tissues. Five programs (NormFinder, geNorm, BestKeeper, RefFinder, and ΔCt) were used to validate the suitability of candidate reference genes. The results revealed that the most appropriate sets of reference genes were RPL10 and ACT across phoxim; ACT and TUB across chlorpyrifos and chlorfluazuron; EF1α and TUB across imidacloprid; EF1α and EF2α across developmental stages; RPL10 and TUB across larvae; EF1α and ACT across tissues, and ACT and G6PDH across sex. These results will facilitate the standardization of RT-qPCR and contribute to further research on B. odoriphaga gene function under insecticides stress.

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Mating triggers immediate upregulation of the heat shock response but downregulation of immune activity in the female reproductive tract of moths

Wang

Gao

et al. 2022

Journal of Asia-Pacific Entomology

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Combined transcriptomic, proteomic and genomic analysis identifies reproductive-related proteins and potential modulators of female behaviors in Spodoptera litura

Cited by 18 publications

References 83 publications

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Selection and Validation of Reference Genes for RT-qPCR Normalization in Bradysia odoriphaga (Diptera: Sciaridae) Under Insecticides Stress

Mating triggers immediate upregulation of the heat shock response but downregulation of immune activity in the female reproductive tract of moths

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