In this paper, we investigate how to cancel multi-user interference without feedback amount over multiple-input multiple-output (MIMO) interference channel using space-time code. Three transmitter-receiver pairs are considered and each node is equipped with four antennas. Space-time codeword with dimension 4 × 4 is designed for three transmitters, respectively. The number of interfering codeword is reduced through the design of codeword. Then, multi-user interference is cancelled by a linear operation on the received signals. Compared with the existing scheme for the same scene, the transmission efficiency is improved, and the unidirectional cooperative link is not required. Simulation results demonstrate the validity of theoretical analysis.