Combining lipidomics and efficacy‐oriented compatibility revealed that Qi Ge decoction compatibility improved lipid metabolism in hyperlipidemic rats

Abstract

The mechanism underlying traditional Chinese medicine (TCM) compatibility is difficult to understand. This study combined lipidomics and efficacy-oriented compatibility to explore underlying compatibility mechanisms of Qi Ge decoction (QG) for improving lipid metabolism in hyperlipidemic rats. The QG was divided into three groups according to the efficacy group strategy: the Huangqi-Gegen (HG), Chenpi (CP), and QG groups. Hyperlipidemic rats were treated with QG, HG, CP, or atorvastatin for 3 weeks. The mass spectral data of widely targeted lipidomics were used to evaluate lipid changes. Principal component analysis and orthogonal partial least squares discriminant analysis were used to assess the lipidomic differences between the groups. MetaboAnalyst 5.0 was used to explore metabolic pathways. Compared with the model group, serum cholesterol, triglyceride, and hepatic steatosis were significantly reduced by QG, whereas HG and CP had no significant effects on these indexes. Lipidomics showed that QG, HG, and CP back-regulated 60, 11, and 14 lipids, respectively. Compared with HG and CP, QG had more metabolic targets in diglycerides, triglycerides, ceramides, and phosphatidylethanolamines. Pathway analysis indicated that QG mainly regulated glycerophospholipid and glycerolipid metabolism.This study provided a new method of combining lipidomics and efficacy-oriented compatibility for exploring the scientific connotation of TCM compatibility.

An assessment of the antihyperlipidemic ingredients of Qi Ge Decoction based on metabolomics combined with serum pharmacochemistry

“…Preliminary research has established a QG fingerprint (Ke et al, 2017), determined the optimal ratio and extraction process (Huang et al, 2017;Li, 2016), identified its main components (Li et al, 2022;Yu et al, 2021), and confirmed its pharmacological efficacy (Zhou, 2021;Zhou et al, 2021;Zhu, 2019). QG has lipid-lowering and hepatoprotective effects in hyperlipidemic rats (Fan et al, 2022(Fan et al, , 2023Peng et al, 2019Peng et al, , 2023, but its effective substances and their targets of action remain unclear.…”