Combining micro- and portable-XRF as a tool for fast identification of virus infections in plants: The case study of ASa-Virus in Fraxinus ornus L.

Porfido, Carlo; Köpke, Kira; Allegretta, Ignazio; Bandte, Martina; Bargen, Susanne von; Rybak, Malgorzata; Falkenberg, Gerald; Mimmo, Tanja; Cesco, Stefano; Büttner, Carmen; Terzano, Roberto

doi:10.1016/j.talanta.2023.124680

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…X-ray fluorescence (XRF) spectroscopy, a qualitative and quantitative technique for the simultaneous analysis of multiple elements, has the advantage of non-destructive analysis and is well suited for the identification and analysis of the essential and toxic elements in plants (Singh et al, 2022), foods (Li et al, 2022) and biological samples (Figueroa et al, 2022). The distribution of elements in plants (Porfido et al, 2023), such as in roots (Zandi et al, 2023) and leaves (Nayak et al, 2011), can be mapped using m-XRF and has been widely used in agricultural, forestry and environmental research (Marguı ´et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

Sun,

Yang,

Luo

2023

J Synchrotron Radiat

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Vegetables are crucial to a human diet as they supply the body with essential vitamins, minerals, etc. Heavy metals that accumulate in plants consequently enter the food chain and endanger people's health. Studying the spatial distribution and chemical forms of elements in plant/vegetable tissues is vital to comprehending the potential interactions between elements and detoxification mechanisms. In this study, leek plants and soil from vegetable gardens near lead–zinc mines were collected and cultivated with 500 mg L−1 PbNO3 solutions for three weeks. Micro X-ray fluorescence was used to map the distribution of Pb and other chemical elements in leek roots, and X-ray absorption near-edge spectroscopy was used to assess the Pb speciation in leek roots and leaves. These findings demonstrated that Pb, Cu, Mn, Cr, Ti and Fe were detected in the outer rings of the root's cross section, and high-intensity points were observed in the epidermis. Zn, K and Ca, on the other hand, were distributed throughout the root's cross section. Leek root and leaf contained significant quantities of lead phosphate and basic lead carbonate at more than 80%, followed by lead sulfide (19%) and lead stearate (11.1%). The capacity of leek roots to convert ambient lead into precipitated lead and fix it on the root epidermis and other inner surfaces is a key mechanism for reducing the toxic effects of Pb.

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Section: Introductionmentioning

confidence: 99%

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

Sun,

Yang,

Luo

2023

J Synchrotron Radiat

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Sample Preparation for X‐Ray Fluorescence Analysis

Marguí,

Queralt

2024

Encyclopedia of Analytical Chemistry

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Forest Tree Virome as a Source of Tree Diseases and Biological Control Agents

Vainio,

Rumbou,

Diez

et al. 2024

Curr. For. Rep.

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Purpose of Review The collective virome of forest trees can be considered to include not only plant viruses, but also viral communities harbored by all tree-associated organisms. In this review, we will concentrate on reviewing recent developments in the two fields of forest tree virology that have received the most research input during the last 5 years: (1) current knowledge of virus diseases affecting forest trees and their causal agents and (2) fungal viruses (mycoviruses) and properties that are required for utilizing them for biocontrol purposes. Recent Findings The discovery of plant and mycoviruses has been revolutionized during the last few years due to high-throughput sequencing (HTS). This has altered our view of virus diversity and prevalence, but also their host ranges, transmission routes, and host effects. Utilization of HTS has greatly expanded our knowledge of plant virus diversity and disease etiology in forest trees and revealed the commonness of cross-kingdom transmission events between fungi, oomycetes, plants, and arthropods. Research on mycoviruses has also identified several new mycoviruses that restrict the growth or virulence of forest pathogenic fungi. Summary Gaining knowledge of the collective virome of forest ecosystems is essential not only for understanding virus evolution and diversity but also for improving our understanding on virus impacts, and our ability for biocontrol-based and environmentally friendly management of viral and fungal diseases that affect economically important plants and beneficial insects, and for preventing possible disease outbreaks in the future. Virus infections play a central role in plant health, but viral symptoms on forest trees remain often unrecognized and may be confused with other biotic or abiotic damages. However, recent studies have revealed previously unknown viruses as causes of forest tree symptoms and suggest that viruses are responsible for far greater economic losses than recognized earlier. However, many knowledge gaps still need to be filled, particularly on the diversity of viruses that infect different species of forest trees, their irregular distribution within the plant, their mode of transmission, epidemiology and choice of hosts also regarding crop plants, their effect on the metabolism of their host tree, and their interaction with other microorganisms. Mycovirus research has already deciphered detailed information on many critical properties that affect utilizing them for biocontrol purposes. Still, more knowledge is needed concerning mycoviral transmission mode and stability in field conditions, the level of host tolerance against mycoviral infection, and the occurrence of interspecies mycovirus transmission in nature, and safety issues related to these topics.

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Combining micro- and portable-XRF as a tool for fast identification of virus infections in plants: The case study of ASa-Virus in Fraxinus ornus L.

Cited by 5 publications

References 34 publications

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

Sample Preparation for X‐Ray Fluorescence Analysis

Forest Tree Virome as a Source of Tree Diseases and Biological Control Agents

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