Comments on the elevated IL-27 expression in neonates: Relevance between detecting nucleotide sequence or protein synthesized

Antas, Paulo Renato Zuquim; Lima, J.W. Marangon; Pedro, Thaíze Q.C.; Silva, Fernanda C.; Ponte, Carlos G.G.

doi:10.1016/j.imlet.2015.01.002

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…Therefore, we sought to determine whether IL‐27 and its receptor are preferentially present in the brain of MS patients. As IL‐27 mRNA levels do not always predict protein expression (Antas et al, ), we performed immunohistochemistry to detect IL‐27 protein subunits on brain tissues obtained from MS patients and controls (Table ). Brain sections were stained for p28 (green) and EBI‐3 (red) and adjacent sections were used for appropriate isotype controls.…”

Section: Resultsmentioning

confidence: 99%

Production of IL‐27 in multiple sclerosis lesions by astrocytes and myeloid cells: Modulation of local immune responses

Sénécal

Deblois

Beauseigle

et al. 2015

Glia

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The mechanisms whereby human glial cells modulate local immune responses are not fully understood. Interleukin-27 (IL-27), a pleiotropic cytokine, has been shown to dampen the severity of experimental autoimmune encephalomyelitis, but it is still unresolved whether IL-27 plays a role in the human disease multiple sclerosis (MS). IL-27 contribution to local modulation of immune responses in the brain of MS patients was investigated. The expression of IL-27 subunits (EBI3 and p28) and its cognate receptor IL-27R (the gp130 and TCCR chains) was elevated within post-mortem MS brain lesions compared with normal control brains. Moreover, astrocytes (GFAP(+) cells) as well as microglia and macrophages (Iba1(+) cells) were important sources of IL-27. Brain-infiltrating CD4 and CD8 T lymphocytes expressed the IL-27R specific chain (TCCR) implying that these cells could respond to local IL-27 sources. In primary cultures of human astrocytes inflammatory cytokines increased IL-27 production, whereas myeloid cell inflammatory M1 polarization and inflammatory cytokines enhanced IL-27 expression in microglia and macrophages. Astrocytes in postmortem tissues and in vitro expressed IL-27R. Moreover, IL-27 triggered the phosphorylation of the transcription regulator STAT1, but not STAT3 in human astrocytes; indeed IL-27 up-regulated MHC class I expression on astrocytes in a STAT1-dependent manner. These findings demonstrated that IL-27 and its receptor were elevated in MS lesions and that local IL-27 can modulate immune properties of astrocytes and infiltrating immune cells. Thus, therapeutic strategies targeting IL-27 may influence not only peripheral but also local inflammatory responses within the brain of MS patients.

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Section: Resultsmentioning

confidence: 99%

Production of IL‐27 in multiple sclerosis lesions by astrocytes and myeloid cells: Modulation of local immune responses

Sénécal

Deblois

Beauseigle

et al. 2015

Glia

View full text Add to dashboard Cite

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Human neonates display altered ex vivo monokine production related to healthy adults

et al. 2016

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The inflammatory response plays an important role during the induction of several neonatal diseases. Previous studies have shown that during newborn infections, the natural imbalance between pro- and anti-inflammatory responses shifts toward the production of pro-inflammatory cytokines. In this study, we employed an array system to detect 9 pro- and anti-inflammatory cytokines, and performed ELISA for 6 other cytokines. We then compared the immune response profiling in umbilical cord blood (UV) plasma samples with circulating levels in otherwise healthy donors (HD). Concentrations of ex vivo monokine levels, such as interleukins (IL)-18, IL-23 and IL-27, were profoundly reduced in the UV in relation to the HD group (p-values of 0.003, 0.009 and <0.0001, respectively). Conversely, UV-plasmatic TGF-β1 levels displayed marked enhancement (p-value=0.005) in relation to HD. Several factors may be implicated in these neonatal alterations, and additional characterization of a broader cytokine panel is warranted to reveal other possible candidates.

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Comments on the elevated IL-27 expression in neonates: Relevance between detecting nucleotide sequence or protein synthesized

Cited by 2 publications

References 5 publications

Production of IL‐27 in multiple sclerosis lesions by astrocytes and myeloid cells: Modulation of local immune responses

Production of IL‐27 in multiple sclerosis lesions by astrocytes and myeloid cells: Modulation of local immune responses

Human neonates display altered ex vivo monokine production related to healthy adults

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