Commercial Microalgal Cultivation Systems

Olaizola, Miguel; Grewe, Claudia

doi:10.1007/978-3-030-25233-5_1

Cited by 26 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was not planned for the station to use a temperature regulation system. However, the system used for monitoring temperature allowed for controlling it, which is very important for maintaining the highest possible productivity [19]. The results of measurements collected over the course of experiments confirmed that the work of the station in an air-conditioned room at a constant temperature of 22 • C prevents the upper-temperature threshold from being exceeded, as proposed by other authors, e.g., in [20,21].…”

Section: Discussionsupporting

confidence: 74%

See 1 more Smart Citation

Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

Borowiak

Pstrowska

Wiśniewski³

et al. 2020

Applied Sciences

View full text Add to dashboard Cite

An increasing number of microalgae strains are used for commercial production of metabolites. When conducting research, the moment of the process scaling tends to be very difficult. One of the most complex issues is related to planning and designing an efficient system for propagation of appropriately high amounts of inoculum required for inoculating cultures on a semi-technical and industrial scale. The following paper aimed at designing an automated station for the preparation of microalgae inoculation material intended for inoculation of the system, comprising of six 90 dm3 volume photobioreactors. The system, comprised of eight airlift photobioreactors of 12 dm3 volume each, installed in mobile storage units connected to the control system in the form of a docking station. Each of the photobioreactors had a separate system used for monitoring temperature and pH, mixing, and LED lighting. The station constituted the last stage of preparing the inoculation material for inoculating technical-scale photobioreactors, used for conducting experiments with Haematococcus pluvialis microalgae. Achieved results, repeatability of the processes, and the ergonomics of the station increased the productivity and quality of the research and development processes.

show abstract

Section: Discussionsupporting

confidence: 74%

“…Providing carbon dioxide ensures simultaneously access to the source of carbon and adjustment of the pH value of the culture medium. This method works exceptionally well in vertical photobioreactors with aeration [19].…”

Section: Discussionmentioning

confidence: 95%

Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

Borowiak

Pstrowska

Wiśniewski³

et al. 2020

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…The FAs profiled for these different microalgae were consistent with what has been reported in previous studies for each alga, concerning FA, but with differences in the FA contents [ 31 , 51 , 52 , 53 , 54 , 55 , 56 ], namely the present work reports higher amounts of ω-3 FA. These differences may be due to the different growth conditions for the microalgae studied, the use of different extraction methodologies (e.g., using different solvents and mixtures of solvents), different derivatization methodologies, or different platforms for data acquisition (e.g., GC-FID) [ 57 , 58 ]. Despite a few common FA species, each microalga had a unique FA signature ( Table 2 ), with microalgae from the same phylum showing similar profiles and clustering at higher levels, as observed in the principal component analysis (PCA) ( Figure 2 ) and in the hierarchical clustering analysis ( Figure S1 ).…”

Section: Discussionmentioning

confidence: 99%

Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity

et al. 2021

View full text Add to dashboard Cite

The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.

show abstract

“…Tubular photobioreactors are an important type among closed photobioreactor systems used for the phototrophic production of microalgal biomass worldwide (Takache et al, 2009;Grewe and Griehl, 2013;Karemore et al, 2015;Olaizola and Grewe, 2019). Biomass productivity and hence space-time yield (often referred to as volumetric yield) of a photoautotrophic bioprocess are influenced by many abiotic factors, which also change during the course of the day as well as throughout the cultivation cycle (Bernard et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Experimental and Model-Based Analysis to Optimize Microalgal Biomass Productivity in a Pilot-Scale Tubular Photobioreactor

Weise

Grewe²,

Pfaff

2020

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

A dynamic coarse-grained model of microalgal growth considering light availability and temperature under discontinuous bioprocess operation was parameterized using experimental data from 15 batch cultivations of Nannochloropsis granulata in a pilot-scale tubular photobioreactor. The methodology applied consists of a consecutive two-step model parameter estimation using pooled, clustered and reorganized data to obtain initial estimates and multi-experiment fitting to obtain the final estimates, which are: maximum specific growth rate µ max = 1.56 d −1 , specific photon half-saturation constant K S,ph = 1.89 mol ph g X −1 d −1 , specific photon maintenance coefficient m ph = 0.346 mol ph g X −1 d −1 and the cardinal temperatures T min = 2.3 • C, T opt = 27.93 • C and T max = 32.59 • C. Biomass productivity prediction proved highly accurate, expressed by the mean absolute percent error MAPE = 7.2%. Model-based numerical optimization of biomass productivity for repeated discontinuous operation with respect to the process parameters cultivation cycle time, inoculation biomass concentration and temperature yielded productivity gains of up to 35%. This optimization points to best performance under continuous operation. The approach successfully applied here to small pilot-scale confirms an earlier one to lab-scale, indicating its transferability to larger scale tubular photobioreactors.

show abstract

Commercial Microalgal Cultivation Systems

Cited by 26 publications

References 44 publications

Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity

Experimental and Model-Based Analysis to Optimize Microalgal Biomass Productivity in a Pilot-Scale Tubular Photobioreactor

Contact Info

Product

Resources

About