Common Tag STSs in the AZF Region Associated with Azoospermia and Severe Oligospermia in Infertile Egyptian Men~!2009-12-21~!2010-04-13~!2010-06-18~!

Abstract

Abstract:Screening of Yq has become one of the most frequently performed postnatal molecular genetic tests in Egypt. Our purpose was to determine the tag sequence-tagged sites (STSs) in the AZF -region of Yq associated with azoospermia and severe oligospermia in infertile Egyptian men. We analyzed blood samples from 49 infertile men (28 with azoospermia and 21 with severe oligospermia) using multiplex PCR for six common AZFa, AZFb, and AZFc STS markers,, as recommended by the European Academy of Andrology. Twenty-four (37%) microdeletions with five separate deletions were identified. We found 66.7% of the deletions in the AZFb locus, 20.8% in the AZFa locus, and 12.5% in the AZFc locus. Some common haplotypes (7 of 10) were identified in our sample population. Haplotypes H3 (corresponding to sY127) and H4 (corresponding to sY134) were the most common. We suggest that screening with a minimum of three STSs-sY86, sY127, and sY134-would provide the highest level of clinical sensitivity in genetic testing among infertile Egyptian men. Moreover, separate microdeletions were localized in infertile Y-chromosome patients.

Detection of partial and/or complete Y chromosome microdeletions of azoospermia factor a (AZFa) sub‐region in infertile Iraqi patients with azoospermia and severe oligozoospermia

“…These findings were in agreement with the findings of Dutta et al 27 and in contrast with the results of Elsaid et al 26 Also, in line with our findings, Sha et al 28 from China, Asadi et al 30 from Iran, and Kim et al 31 from Korea, reported higher prevalence of AZFa microdeletions in patients with azoospermia than in those with oligozoospermia. Although in this study, AZFa microdeletion was found in both groups, but in previous studies from Egypt, 16 , 32 India, 27 China, 29 Iran, 30 and Korea, 31 it was identified only in the azoospermia patients, which was contrary to this research.…”

Y-chromosome microdeletions and the MTHFR C677T polymorphism in Egyptian men with nonobstructive azoospermia

“…However, a study carried out in 2010 in Egypt on 49 patients reported a much higher percentage of microdeletions (37%) and the highest frequency of microdeletions was in the AZFb locus, followed by the AZFa locus and then the AZFc locus [32]. This was not in agreement with the results of the present study and many other studies that reported the highest frequency of microdeletion in the AZFc locus [33,34].…”

Prevalence of Y chromosome microdeletions in infertile Tunisian men

“…The low frequency of the AZF microdeletions in our cohort is in accordance with some previous studies reported from Algeria (1.3%) [10], Sri Lanka (1.45%) [11] and Iran (1.74%) [12]. However, this rate is lower than the frequencies reported from Morocco (3.1%) [25], Turkey (3.3 and 6%) [13,28], Lativia (4.8%) [26], Kuwait (7.75%) [14], China (8.9 and 19,4%) [15,16], Serbia (15.5%) [17], and Egypt (36.7%) [18] (table 1).…”