Common themes in centriole and centrosome movements

Vaughan, Sue; Dawe, Helen R.

doi:10.1016/j.tcb.2010.09.004

Cited by 33 publications

(29 citation statements)

References 98 publications

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“…Astral microtubules connect centrosomes to the cell cortex and apply pulling forces to the cortical cytoskeleton during mitosis (Manneville and Etienne-Manneville 2006;Kunda and Baum 2009;Vaughan and Dawe 2010). Cortical actin patches may localize microtubule anchoring and/or motor proteins such as APC2 and dynein to counteract such forces (McCartney et al 2001;Sandquist et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Merlin/ERM proteins establish cortical asymmetry and centrosome position

et al. 2012

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The ability to generate asymmetry at the cell cortex underlies cell polarization and asymmetric cell division. Here we demonstrate a novel role for the tumor suppressor Merlin and closely related ERM proteins (Ezrin, Radixin, and Moesin) in generating cortical asymmetry in the absence of external cues. Our data reveal that Merlin functions to restrict the cortical distribution of the actin regulator Ezrin, which in turn positions the interphase centrosome in single epithelial cells and three-dimensional organotypic cultures. In the absence of Merlin, ectopic cortical Ezrin yields mispositioned centrosomes, misoriented spindles, and aberrant epithelial architecture. Furthermore, in tumor cells with centrosome amplification, the failure to restrict cortical Ezrin abolishes centrosome clustering, yielding multipolar mitoses. These data uncover fundamental roles for Merlin/ERM proteins in spatiotemporally organizing the cell cortex and suggest that Merlin's role in restricting cortical Ezrin may contribute to tumorigenesis by disrupting cell polarity, spindle orientation, and, potentially, genome stability.

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Section: Discussionmentioning

confidence: 99%

Merlin/ERM proteins establish cortical asymmetry and centrosome position

et al. 2012

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“…Centrosome dynamics play key roles in the regulation of mitotic and non-mitotic cell function (Vaughan and Dawe, 2010). We have uncovered a novel type of asymmetric centrosome dynamics during cytokinesis of asymmetrically dividing progenitor cells.…”

Section: Discussionmentioning

confidence: 99%

Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

et al. 2013

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SUMMARYAsymmetric cell division generates cell fate diversity during development and adult life. Recent findings have demonstrated that during stem cell divisions, the movement of centrosomes is asymmetric in prophase and that such asymmetry participates in mitotic spindle orientation and cell polarization. Here, we have investigated the dynamics of centrosomes during Drosophila sensory organ precursor asymmetric divisions and find that centrosome movements are asymmetric during cytokinesis. We demonstrate that centrosome movements are controlled by the cell fate determinant Numb, which does not act via its classical effectors, Sanpodo and α-Adaptin, but via the Collapsin Response Mediator Protein (CRMP). Furthermore, we find that CRMP is necessary for efficient Notch signalling and that it regulates the duration of the pericentriolar accumulation of Rab11-positive endosomes, through which the Notch ligand, Delta is recycled. Our work characterizes an additional mode of asymmetric centrosome movement during asymmetric divisions and suggests a model whereby the asymmetry in centrosome movements participates in differential Notch activation to regulate cell fate specification.

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“…The centrosome functions as the main MT-organizing center for cells and has key roles in many biological processes (Vaughan and Dawe, 2011), including neurogenesis and neuronal migration (Higginbotham and Gleeson, 2007). However, conflicting evidence has come from studies of centrosome function in axon development.…”

Section: Introductionmentioning

confidence: 99%

Centrosome movements in vivo correlate with specific neurite formation downstream of LIM homeodomain transcription factor activity

Andersen

Halloran

2012

Development

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SUMMARYNeurons must develop complex structure to form proper connections in the nervous system. The initiation of axons in defined locations on the cell body and their extension to synaptic targets are critical steps in neuronal morphogenesis, yet the mechanisms controlling axon formation in vivo are poorly understood. The centrosome has been implicated in multiple aspects of neuronal morphogenesis; however, its function in axon development is under debate. Conflicting results from studies of centrosome function in axonogenesis suggest that its role is context dependent and underscore the importance of studying centrosome function as neurons develop in their natural environment. Using live imaging of zebrafish Rohon-Beard (RB) sensory neurons in vivo, we discovered a spatiotemporal relationship between centrosome position and the formation of RB peripheral, but not central, axons. We tested centrosome function by laser ablation and found that centrosome disruption inhibited peripheral axon outgrowth. In addition, we show that centrosome position and motility are regulated by LIM homeodomain transcription factor activity, which is specifically required for the development of RB peripheral axons. Furthermore, we show a correlation between centrosome mislocalization and ectopic axon formation in bashful (laminin alpha 1) mutants. Thus, both intrinsic transcription factor activity and extracellular cues can influence centrosome position and axon formation in vivo. This study presents the first positive association between the centrosome and axon formation in vivo and suggests that the centrosome is important for differential neurite formation in neurons with complex axonal morphologies.

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Common themes in centriole and centrosome movements

Abstract: 11Centrioles are found in nearly all eukaryotic cells and are required for growth and 12 maintenance of the radial array of microtubules, the mitotic spindle, and cilia and flagella.

Cited by 33 publications

References 98 publications

Merlin/ERM proteins establish cortical asymmetry and centrosome position

Merlin/ERM proteins establish cortical asymmetry and centrosome position

Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

Centrosome movements in vivo correlate with specific neurite formation downstream of LIM homeodomain transcription factor activity

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