Communications Between the Nuclotide-and Actin-Binding Site of the Myosin Head in Muscle Fibers

A review of TNP-ATP in protein binding studies: benefits and pitfalls

“…TNP-ATP has long been used, and continues to be used, to study how ATP interacts with individual proteins ( 3 ). TNP-ATP fluorescence is used in fluorescence microscopy to visualize the cellular location of proteins ( 14 , 15 , 16 , 17 , 18 ) and as a Förster or fluorescence resonance energy transfer (FRET) pair ( 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ). Many physical properties of TNP-ATP, including absorption and fluorescence, have been previously reported and reviewed ( 3 , 13 ).…”