Compact adaptive optics system for multiphoton fundus imaging

Bille, Josef F.; Agopov, Mikael; Alvarez-diez, Cristina; Han, Meng; Korablinova, Nina; Pape, Ulrich; Schiazza, Olivier La; Schwingel, Melanie; Zhang, Hongwei; Müller, Frank

doi:10.1080/09500340701608024

Cited by 5 publications

(7 citation statements)

References 13 publications

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“…Parameters such as cell density and inter-distance have been analyzed for different eccentricities in order to provide additional information on cellular arrangement. Although retinal cells have been imaged with this microscopy technique [20][21][22][24][25][26][27], to our knowledge this is the first time that TPEF images have been used to compare densities and spacial distributions between myopic and emmetropic eyes.…”

Section: Discussionmentioning

confidence: 99%

“…Since Imanishi and colleagues reported that both the retinal pigment epithelium (RPE) [18] and the PR outer segments [19] of the mouse retina provided TPEF signal, different authors have explored the visualization and distribution of retinal cells in animal models [20][21][22] and humans [23][24][25][26]. In particular, the combination of AO and multiphoton microscopy provided improved images of every retinal layer in nonstained human and chicken retinas [16,23,27].…”

Section: Introductionmentioning

confidence: 99%

“…Although chicken retinal cells have recently been imaged in vivo [25,26], there are few data on the effects of induced myopia on retinal cell spacing, distribution and density in chickens, except for a histological study on the spacing and arborization of retinal beta ganglion cells [29]. Moreover, PR densities have not been mapped out.…”

Section: Introductionmentioning

confidence: 99%

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Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy

Bueno¹,

Palacios²,

Giakoumaki³

et al. 2014

Biomed. Opt. Express

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Abnormal eye growth induced by visual deprivation can modify the structure and density of the retinal cells. We have used an adaptive optics multiphoton microscope to image photoreceptors (PRs) and ganglion cells (GCs) at different retinal locations in unstained retinas of chicken eyes with about 10D of myopia and their normal-sighted fellow eyes. In all samples, the local averaged inter-PR distance increased with eccentricity. No significant differences in PR density were found between control and myopic eyes. GC density declined in myopic eyes compared to control eyes and the inter-cell distance increased. In normal eyes, the size of the GC cell bodies increased approximately two-fold between the area centralis and the peripheral retina. In myopic eyes, this trend was preserved but the GC bodies were larger at each retinal location, compared to control eyes. Obviously, GC morphology is changing when the retinal area is enlarged in myopic eyes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy

Bueno¹,

Palacios²,

Giakoumaki³

et al. 2014

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…And this leads to wavefront designed glasses, contact lenses, intraocular implants, and wavefront-guided laser vision correction. Also, fundus cameras, SLO, Optical coherence tomography (OCT) and two-photon ophthalmoscopy (TPO) have incorporated adaptive optics to achieve diffraction-limited imaging system [9][10][11].…”

Section: Brief History Of Adaptive Opticsmentioning

confidence: 99%

“…Further, the OCT has also been integrated with adaptive optics for aberration-free retinal imaging to improve the axial and lateral resolution. Adaptive optics was integrated with ultrahigh resolution OCT and spectral domain OCT [10,23].Likewise, the adaptive optics has been incorporated with TPO aimed at detecting the early onset of retinal diseases [9]. In Fig.…”

Section: Aberration-free Retinal Imagingmentioning

confidence: 99%