Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs

Putame, Giovanni; Gabetti, Stefano; Carbonaro, Dario; Meglio, Franca Di; Romano, Veronica; Sacco, Anna Maria; Belviso, Immacolata; Serino, Gianpaolo; Bignardi, Cristina; Morbiducci, Umberto; Castaldo, Clotilde; Massai, Diana Nada Caterina

doi:10.1016/j.medengphy.2020.07.018

Cited by 19 publications

(12 citation statements)

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“…In order to create PDL-cementum complexes, it becomes paramount to tailor the cell environment (mimicking the ECM) by selecting the best scaffold capable of supporting PDLSC growth and differentiation in humans, which implies the adoption of the most up-to-date 3D printing techniques. In parallel, the biomimetic approach demands the use of technological devices for supporting the in vitro maturation of the tissue to be grafted, particularly bioreactors providing controlled physical stimuli to reproduce the native environment [ 167 , 168 , 169 ]. Indeed, mechanical stimulation can be essential for promoting specific cellular and tissue processes with the final aim of inducing the correct orientation of the fibers forming the PDL and of maintaining tissue homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Challenges of Periodontal Tissue Engineering: Increasing Biomimicry through 3D Printing and Controlled Dynamic Environment

et al. 2022

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In recent years, tissue engineering studies have proposed several approaches to regenerate periodontium based on the use of three-dimensional (3D) tissue scaffolds alone or in association with periodontal ligament stem cells (PDLSCs). The rapid evolution of bioprinting has sped up classic regenerative medicine, making the fabrication of multilayered scaffolds—which are essential in targeting the periodontal ligament (PDL)—conceivable. Physiological mechanical loading is fundamental to generate this complex anatomical structure ex vivo. Indeed, loading induces the correct orientation of the fibers forming the PDL and maintains tissue homeostasis, whereas overloading or a failure to adapt to mechanical load can be at least in part responsible for a wrong tissue regeneration using PDLSCs. This review provides a brief overview of the most recent achievements in periodontal tissue engineering, with a particular focus on the use of PDLSCs, which are the best choice for regenerating PDL as well as alveolar bone and cementum. Different scaffolds associated with various manufacturing methods and data derived from the application of different mechanical loading protocols have been analyzed, demonstrating that periodontal tissue engineering represents a proof of concept with high potential for innovative therapies in the near future.

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Section: Discussionmentioning

confidence: 99%

Challenges of Periodontal Tissue Engineering: Increasing Biomimicry through 3D Printing and Controlled Dynamic Environment

et al. 2022

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“…For applying isotonic cyclic load to the ECTs, the bioreactor can be equipped with an actuation system driven by a captive stepper motor (NEMA 14, Nanotec Electronic GmbH & Co. KG, Feld- kirchen, DE) that generates a linear motion with a resolution of 10 μm/step ( Putame et al., 2020 ). The stepper motor controls the cyclic translation of the mechanical stimulation module’s comb-like structure, in which the stainless steel wires are fixed.…”

Section: Methodsmentioning

confidence: 99%

Bizonal cardiac engineered tissues with differential maturation features in a mid-throughput multimodal bioreactor

et al. 2022

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“…RNA obtained was resuspended in RNase-free water and the final concentration was determined by spectrophotometric analysis with Nanodrop 2000 (Thermo Scientific). RNA extracted was then retrotranscribed with QuantiTect Reverse Transcription kit (Qiagen) following the protocol provided by the supplier as previously described ( Putame et al, 2020 ). In order to assess the presence and the quantity of specific transcripts, a Real-Time PCR was performed using PrecisionPLUS TM MasterMix kit (Primerdesign, Southampton, United Kingdom) according to manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro

Romano¹,

Belviso²,

Sacco³

et al. 2022

Front. Physiol.

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Although human Cardiac Progenitor Cells (hCPCs) are not retained by host myocardium they still improve cardiac function when injected into ischemic heart. Emerging evidence supports the hypothesis that hCPC beneficial effects are induced by paracrine action on resident cells. Extracellular vesicles (EVs) are an intriguing mechanism of cell communication based on the transport and transfer of peptides, lipids, and nucleic acids that have the potential to modulate signaling pathways, cell growth, migration, and proliferation of recipient cells. We hypothesize that EVs are involved in the paracrine effects elicited by hCPCs and held accountable for the response of the infarcted myocardium to hCPC-based cell therapy. To test this theory, we collected EVs released by hCPCs isolated from healthy myocardium and evaluated the effects they elicited when administered to resident hCPC and cardiac fibroblasts (CFs) isolated from patients with post-ischemic end-stage heart failure. Evidence emerging from our study indicated that hCPC-derived EVs impacted upon proliferation and survival of hCPCs residing in the ischemic heart and regulated the synthesis and deposition of extracellular-matrix by CFs. These findings suggest that beneficial effects exerted by hCPC injection are, at least to some extent, ascribable to the delivery of signals conveyed by EVs.

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Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs

Cited by 19 publications

References 65 publications

Challenges of Periodontal Tissue Engineering: Increasing Biomimicry through 3D Printing and Controlled Dynamic Environment

Challenges of Periodontal Tissue Engineering: Increasing Biomimicry through 3D Printing and Controlled Dynamic Environment

Bizonal cardiac engineered tissues with differential maturation features in a mid-throughput multimodal bioreactor

Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac Repair in Vitro

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