In the dynamic and rapid detection of retroreflection coefficient in road traffic markings, different factors may affect the detection results. This study investigates the significance of different levels of environmental light intensity and vehicle speed on the detection results of retroreflection coefficient in road markings using one-way ANOVA analysis. The results show that within a 95% confidence interval, there is no statistically significant difference in the detection results of retroreflection coefficient under different levels of environmental light intensity and vehicle speed. The variance effect size calculation indicates that for the detection of retroreflection coefficient in road markings, vehicle speed control is more important than light intensity. To obtain more reliable detection results, it is recommended to maintain a stable and uniform speed of no more than 80 km/h during the detection process.