Comparability of Lipoprotein Particle Number Concentrations Across ES-DMA, NMR, LC-MS/MS, Immunonephelometry, and VAP: In Search of a Candidate Reference Measurement Procedure for apoB and non-HDL-P Standardization

Abstract

This study demonstrates that ALT methods do not yet provide equivalent results for the measurement of non-HDL-P and apoB-100. The better agreement between methods harmonized to the WHO/IFCC reference material suggests that standardizing ALT methods by use of a common commutable calibrator will improve cross-platform comparability. This study provides further evidence that LC-MS/MS is the most suitable candidate reference measurement procedure to standardize apoB-100 measurement, as it would provide results with SI traceability. The absence of freezing and thawing effect suggests that frozen serum pools could be used as secondary reference materials.

Quantifying atherogenic lipoproteins for lipid-lowering strategies: consensus-based recommendations from EAS and EFLM

“…The International Federation of Clinical Chemistry (IFCC) and World Health Organization (WHO) developed the SP3 reference material, value-assigned using immunonephelometry as interim reference method for apoB [64]. Common calibration with the IFCC/WHO SP3 reference material has reduced between-laboratory variability of apoB measurements from >19% to <10%, although concerns about the variability among immunoassays and their comparability with apoB100 derived from NMR and other methods still exist [65][66][67]. Liquid chromatography tandem-mass spectrometry (LC-MSMS)-based quantification of apolipoproteins has the potential to further improve apoB100 standardization and between-method comparability [67].…”

“…Common calibration with the IFCC/WHO SP3 reference material has reduced between-laboratory variability of apoB measurements from >19% to <10%, although concerns about the variability among immunoassays and their comparability with apoB100 derived from NMR and other methods still exist [65][66][67]. Liquid chromatography tandem-mass spectrometry (LC-MSMS)-based quantification of apolipoproteins has the potential to further improve apoB100 standardization and between-method comparability [67]. The IFCC has therefore initiated development of LC-MSMS as candidate definitive reference method for apoB [67].…”

“…Liquid chromatography tandem-mass spectrometry (LC-MSMS)-based quantification of apolipoproteins has the potential to further improve apoB100 standardization and between-method comparability [67]. The IFCC has therefore initiated development of LC-MSMS as candidate definitive reference method for apoB [67]. Another advantage of LC-MSMS is that it enables the simultaneous (multiplexed) measurement of multiple apolipoproteins in addition to apoB100 in a single run of the assay, thus making it possible to achieve a complete apolipoprotein profile in the patient, including HDL-and VLDL-associated apolipoproteins for comprehensive characterization of dyslipidemias [68].…”

Lipid findings from the Diabetes Education to Lower Insulin, Sugars, and Hunger (DELISH) Study

“…Several differences in study design may account for at least some of these discrepant findings. First, we used a different advanced lioprotein assayp (NMR LipoProfile) that is widely accessible but, tends to differ somewhat in results from the methods used by Chiu and colleagues [48]. Second, Chiu and colleagues’ sample was primarily male (85%), whereas ours was primarily female (64%).…”

“…Although this study used the widely available NMR Lipoprofile, such testing is limited because it lacks standardization and comparability of information provided by other forms of advanced lipoprotein assays [51]. For example, after finding that different advanced lipid measurement methods yielded differential non-HDL-P values, Delatour and colleagues [48] recently called for standardizing these methods by use of a common commutable calibrator to improve cross-platform comparability.…”