Comparação do processo de reparo ósseo em tíbias de ratas normais e osteopênicas

Sartori, Angélica Rossi; Moreira, Julieta Aparecida; Santos, Antonio Marcos Martins; Cintra, Dennys Esper; Sartori, Lucas Rossi; Baraúna, Mario Antonio; Canto, Roberto Sérgio Tavares

doi:10.1590/s1413-78522008000100007

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…[28] Although the loose connective tissue and animal species in the study area is very slim, the same degree of interference in the repair of bone tissue was extremely high, preventing the direct action of Cramoll-1,4 on bone tissue.…”

Section: Resultsmentioning

confidence: 99%

Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats

Santos-Oliveira

Lima-Ribeiro

Carneiro-Leão

et al. 2013

Int J App Basic Med Res

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Context:Regeneration corresponds to the replacement of damaged cells with ones that have the same morphology and function. For experimental evaluation of materials that may favor the process of bone healing, defects are created with dimensions that prevent spontaneous regeneration. For the development and use of new drugs, it is necessary to study its effects in vitro, which depends on the formulation, concentration, and rate of irradiation in vivo and the route and frequency of administration; thus, it is possible to characterize the physiological and molecular mechanisms involved in the response and cellular effects.Objective:The objective of this study was to assess the effect of Cramoll-1,4 on the process of bone repair.Materials and Methods:A formulation of biopharmaceutical lectin Cramoll-1,4 at a concentration of 300 mg/100 mL was applied in a single application via gamma radiation and its effect on the process of bone repair in rats was assessed.Results:Histologically, it was observed that the bone defect is coated by loose connective tissue rich in fibroblasts, providing a range similar to the thick bone original and competing with site of new bone formation. This prevented direct contact between the formulation and experimental bone tissue, as, despite its proven effectiveness in experiments on the repair of skin lesions, the formulation used did not promote bone stimulation that would have promoted the tissue repair process.Conclusion:Because of the direct interference of loose tissue repair that prevented direct contact of the implant with the bone interface, the formulation did not promote bone stimulation.

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Section: Resultsmentioning

confidence: 99%

Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats

Santos-Oliveira

Lima-Ribeiro

Carneiro-Leão

et al. 2013

Int J App Basic Med Res

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show abstract

“…Previous studies on osteoporotic fracture healing have demonstrated the influence of osteoporosis in both the initial ( 10 ) and late ( 11 ) bone healing phases. Osteoporosis can speed up the initial healing phase and delay bony callus mineralization that forms between and around the edges of bone fractures ( 12 ), and speeds up the fracture-healing processes ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study

Paiva

Yanagihara

Macedo

et al. 2016

Braz J Med Biol Res

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Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail suspension, when the osteopenic condition was already set. The fractured limb was then immobilized by orthosis. Tibias were collected 2 and 6 weeks after osteotomy, and were analyzed by bone densitometry, mechanical testing, and histomorphometry. Bone mineral density values from bony calluses were significantly lower in the 2-week post-osteotomy groups compared with the 6-week post-osteotomy groups (multivariate general linear model analysis, P<0.000). Similarly, the mechanical properties showed that animals had stronger bones 6 weeks after osteotomy compared with 2 weeks after osteotomy (multivariate general linear model analysis, P<0.000). Histomorphometry indicated gradual bone healing. Results showed that osteopenia did not influence the bone healing process, and that time was an independent determinant factor regardless of whether the fracture was osteopenic. This suggests that the body is able to compensate for the negative effects of suspension.

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A high-fat diet can affect bone healing in growing rats

et al. 2017

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A high-fat diet (HFD) can have a negative effect on bone quality in young and old people. Although bone healing in children is normally efficient, there is no evidence that children who have a diet rich in fat have compromised bone fracture regeneration compared with children with recommended dietary fat levels. The purpose of the present study was to evaluate the effects of an HFD on bone healing in growing female rats. Twenty-six postweaning female Wistar rats were divided into two groups (13 animals per group): a standard diet (SD) group and an HFD (with 60% of energy from fat) group. The rats received the assigned diets for 5 weeks, and in the third week they were submitted to an osteotomy procedure of the left tibia. Body mass and feed intake were recorded during the experiment. One day before euthanasia, an insulin tolerance test was performed. After euthanasia, the tibiae were removed and analyzed by densitometry, mechanical testing, histomorphometry, stereology and immunohistochemistry. An HFD caused an adaptive response to maintain energetic balance by decreasing feed intake and causing insulin insensitivity. There was no change in bone mineral density, collagen amount and immunostaining for bone formation, but maximal load and stiffness were decreased in the HFD group. In addition, bone volume had a tendency to be higher in the SD group than in the HFD group. Compared with rats receiving an SD, growing rats receiving an HFD for 5 weeks had similar bone mineral density but altered mechanical properties at the osteotomy defect site.

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Comparação do processo de reparo ósseo em tíbias de ratas normais e osteopênicas

Cited by 8 publications

References 15 publications

Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats

Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study

A high-fat diet can affect bone healing in growing rats

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