Comparaison de la sensibilité aux antibiotiques de Corynebacterium diphtheriae par les méthodes de dilution en bouillon et de diffusion (E-test et disques)

Benamrouche, Nabila; Lazri, M.; Tali-Maamar, Hassiba; Rahal, K

doi:10.1016/j.medmal.2014.07.007

Cited by 9 publications

(2 citation statements)

References 4 publications

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“…The antimicrobial of essential oils were determined according to the standard agar disk diffusion method with a slight modification ( Luangtongkum et al, 2007 ; Gaudreau et al, 2008 ; Benamrouche et al, 2014 ; Wu et al, 2018 ; Wu et al, 2021 ). A 0.5 McFarland (1 × 10 7 to 1 × 10 8 CFU/mL) concentration of the bacterial suspension was uniformly inoculated onto MHA solidified in 120 mm Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition, Antimicrobial and Insecticidal Activities of Essential Oils of Discarded Perfume Lemon and Leaves (Citrus Limon (L.) Burm. F.) as Possible Sources of Functional Botanical Agents

Tang

Jian

et al. 2021

Front. Chem.

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Two essential oils were isolated from discarded perfume lemon and leaves (Citrus limon (L.) Burm. F.) by hydro-distillation with good yield (0.044% for perfume lemon and 0.338% for leaves). Their biological activities were evaluated against five selected bacterial strains and Aedes albopictus (Ae. albopictus, Diptera: Culicidae). Chemical composition indicated that both essential oils were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpene. These constituents revealed some variability among the oils displaying interesting chemotypes (R)-(+)-limonene (12.29–49.63%), citronellal (5.37–78.70%) and citronellol (2.98–7.18%). The biological assessments proved that the two essential oils had similar effect against bacterial (inhibition zones diameter ranging from 7.27 ± 0.06 to 10.37 ± 0.15 mm; MICs and MBCs ranging from 1.6 to 6.4 mg/mL); against Ae. albopictus larvae (LC50 ranging from 384.81 to 395.09 ppm) and adult mosquito (LD50 ranging from 133.059 to 218.962 μg/cm2); the activity of the two chemotypes ((R)-(+)-limonene and citronellal): larvae (LC50 ranging from 267.08 to 295.28 ppm), which were all presented in dose-dependent manners. Through this work, we have showcased that recycling and reusing of agriculture by-products, such as discarded perfume lemon and leaves can produce eco-friendly alternatives in bacterial disinfectants and mosquito control product.

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Section: Methodsmentioning

confidence: 99%

Chemical Composition, Antimicrobial and Insecticidal Activities of Essential Oils of Discarded Perfume Lemon and Leaves (Citrus Limon (L.) Burm. F.) as Possible Sources of Functional Botanical Agents

Tang

Jian

et al. 2021

Front. Chem.

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“…The antimicrobial activity of WU-FA-00 and WU-FA-01 was determined according to the standard agar disk diffusion method with a slight modification. 33 – 35 A 0.5 McFarland (1×10 7 –1×10 8 CFU/mL) concentration of the bacterial suspension was uniformly inoculated onto MHA solidified in 120 mm Petri dishes. Once the dishes were prepared, 6 mm diameter discs of filter paper containing 5 µL of the examined compound, which had been diluted 10 times with DMSO, were pressed gently against the surface of the agar.…”

Section: Methodsmentioning

confidence: 99%

The biological evaluation of fusidic acid and its hydrogenation derivative as antimicrobial and anti-inflammatory agents

Wu¹,

He²,

Hong³

et al. 2018

IDR

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BackgroundFusidic acid (FA) (WU-FA-00) is the only commercially available antimicrobial from the fusidane family that has a narrow spectrum of activity against Gram-positive bacteria.MethodsHerein, the hydrogenation derivative (WU-FA-01) of FA was prepared and both compounds were examined against a panel of six bacterial strains. In addition, their anti-inflammatory properties were evaluated using a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model.ResultsThe results of the antimicrobial assay revealed that both WU-FA-00 and WU-FA-01 displayed a high level of antimicrobial activity against Gram-positive strains. Moreover, killing kinetic studies were performed and the results were in accordance with the minimum inhibitory concentration and minimum bactericidal concentration results. We also demonstrated that the topical application of WU-FA-00 and WU-FA-01 effectively decreased TPA-induced ear edema in a dose-dependent manner. This inhibitory effect was associated with the inhibition of TPA-induced upregulation of proinflammatory cytokines IL-1β, TNF-α, and COX-2. WU-FA-01 significantly suppressed the expression levels of p65, IκB-α, and p-IκB-α in the TPA-induced mouse ear model.ConclusionOverall, our results showed that WU-FA-00 and WU-FA-01 not only had effective antimicrobial activities in vitro, especially to the Gram-positive bacteria, but also possessed strong anti-inflammatory effects in vivo. These results provide a scientific basis for developing FA derivatives as antimicrobial and anti-inflammatory agents.

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A novel 18β-glycyrrhetinic acid derivative supramolecular self-assembly hydrogel with antibacterial activity

et al. 2021

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Hydrogels are kinds of soft materials with 3D network structure, which play important roles in medical materials, such as contact lenses, surgical implants, wound dressings, artificial skin, drug release carriers, suture coatings, especially antibacterial materials that could solve problems of microbial infections. There are many studies on 18b-glycyrrhetinic acid (GA) derivatives hydrogel, but few on application in antibacterial materials. GA-O-20, one of GA derivatives, showed marvelous antibacterial property compared with its parent compound GA. Therefore, it was adopted as basic functional material to prepare supramolecular self-assembly hydrogels in the mixture solvent of water and ethanol. This hydrogel exhibits outstanding thermodynamic stability; the gel melting temperature (T gel ) is high to 96.6 ± 0.48 °C. Besides, it presented exceptional antibacterial that its minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) value are 6.25 and 12.5 lM, respectively, which was possibility caused by its sustained self-released property. This supramolecular self-assembly hydrogel may have enormous potentials in the application of antibacterial materials and drug delivery, which can be helpful to expand the application of GA derivatives.

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Comparaison de la sensibilité aux antibiotiques de Corynebacterium diphtheriae par les méthodes de dilution en bouillon et de diffusion (E-test et disques)

Cited by 9 publications

References 4 publications

Chemical Composition, Antimicrobial and Insecticidal Activities of Essential Oils of Discarded Perfume Lemon and Leaves (Citrus Limon (L.) Burm. F.) as Possible Sources of Functional Botanical Agents

Chemical Composition, Antimicrobial and Insecticidal Activities of Essential Oils of Discarded Perfume Lemon and Leaves (Citrus Limon (L.) Burm. F.) as Possible Sources of Functional Botanical Agents

The biological evaluation of fusidic acid and its hydrogenation derivative as antimicrobial and anti-inflammatory agents

A novel 18β-glycyrrhetinic acid derivative supramolecular self-assembly hydrogel with antibacterial activity

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