Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis

Abstract

SummaryThis study represents the first genome-wide transcriptomic and lipidomic analysis of H. pluvialis to reveal the regulation of astaxanthin biosynthesis and lipid metabolism during the encystment process under high irradiation.

Optimizing light regimes on growth and lipid accumulation in Ankistrodesmus fusiformis H1 for biodiesel production

“…On the contrary, dramatic reductions in GL and PL were occurred in ML and HL cultures. It was evident to find that higher light intensity was benefit for the storage of NL, and this trend is similar to that of previous reports (Ho et al, 2014;Gwak et al, 2014;He et al, 2015a,b;Xiao et al, 2015). Overall, light regimes were important for NL accumulation in A. fusiformis H1.…”

“…The results was consistent with previous reports in which the lipid content was promoted under HL, mainly in the form of neutral lipid (Gwak et al, 2014;He et al, 2015a,b;Xiao et al, 2015). Summarized the productivities in Table 2 intensity affects the relative size of the lipid pools and the fixation of carbon increased with light, thus, lipid storage can provide a larger sink for the available energy, especially when light was in excess to the requirement for growth (He et al, 2015a,b).…”

“…motisis, the TCA cycle and lipid b-oxidation occur during the dark period (Chauton et al, 2013). Numerous studies have reported the lipid content in microalgae (e.g., Nannochloropsis, Chlorella sp., Monoraphidium dybowskii) can accumulatively increase under higher light intensity (Wahidin et al, 2013;Gwak et al, 2014;He et al, 2015b), but it had seldom reported for Ankistrodesmus sp. The lipid in microalgae primarily constitute of polar structural lipids (such as phospholipids and glycolipids) together with storage neutral lipids especially TAG which is the most important component for biodiesel production (Olofsson et al, 2012).…”

“…Summarized the productivities in Table 2 intensity affects the relative size of the lipid pools and the fixation of carbon increased with light, thus, lipid storage can provide a larger sink for the available energy, especially when light was in excess to the requirement for growth (He et al, 2015a,b). In many literatures, it had been proved that higher light can synchronously accumulate both biomass and lipid to achieve large amounts of lipid products eventually (Damiani et al, 2010;Ho et al, 2014;Gwak et al, 2014;He et al, 2015a,b;Xiao et al, 2015). However, the necessity of light varied in microalgae from one to another, even in same species.…”

Metabolite Profiling and Integrative Modeling Reveal Metabolic Constraints for Carbon Partitioning under Nitrogen Starvation in the Green Algae Haematococcus pluvialis

“…Whereas the initial astaxanthin synthesis utilizes the existing ␤-carotene as a precursor, bulk accumulation of astaxanthin esters will depend on de novo ␤-carotene synthesis (53). In fact, almost all of the genes involved in astaxanthin biosynthesis from isopentenyl pyrophosphate were found to be simultaneously up-regulated in transcriptional expression in high irradiance-induced red cells of H. pluvialis (54). Similarly, high expression levels of enzymes involved in the synthesis of terpenoids were also found in Neochloris oleoabundans, under nitrogen deficiency (55).…”