Article Info Received : 29.04.2017 Accepted : 08.07.2017 Soil microbial communities and their related characteristics are an important agent for soil fertility, productivity, and sustainability. Also, they are useful indicators of soil quality and life index in agricultural systems. The objectives of this study were the effect of nanozeolite and plant residues on soil microbial communities and their characteristics and also, the assessment of incubation timing on soil microbial properties. Soil microorganisms are very important in the decomposition of plant residues. In this regard, the soil samples were treated by nanozeolite (0, 10 and 30% Weight), Alfalfa and wheat straw (0 and 5% Weight). The treated soil samples were incubated in lab condition for 90 days. The result of this study showed that Bacterial, Fungal, and Actinomycete populations increased by the addition of 30% of nanozeolite and 5% of plant residues, especially alfalfa straw. Also, the addition of nanozeolite and plant residues treatments improved MBC, BR, and SIR as microbial characteristics. These parameters increased after 30 days of starting incubation, then decreased until the 75 th day and finally increased slightly on the 90 th day. In fact, the addition of nanozeolite and plant residues into the soil had positive effects on improvement of carbon pools and increasing carbon sequestration in it. Applied nanozeolite and plant residues in soil, improved carbon pools and increased carbon sequestration in soil. Also the application of nanozeolite and plant residues especially alfalfa straw had positive effects on improvement of soil biological communities and characteristics.