Comparative analysis of biofilm models to determine the efficacy of antimicrobials

Besser, Manuela; Brill, Florian H. H.; Geffken, Maria; Plattfaut, Isabell; Severing, Anna-Lena; Wiencke, Vanessa; Rembe, Julian‐Dario; Naumova, Ella A.; Kampe, Andreas; Debus, Sebastian; Smeets, Ralf

doi:10.1016/j.ijheh.2021.113744

Cited by 12 publications

(13 citation statements)

References 30 publications

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“…OCT is known as a highly effective antibacterial agent, 58,59 and our research confirmed that this antimicrobial effect of OCT remains preserved even when OCT is not free but when it is bonded onto polymer surfaces. These results can be important for many purposes when we need the development of new bacteriaresistant material.…”

Section: Resultssupporting

confidence: 74%

Antibacterial nanocomposite supporting cell growth and spheroid formation by chemical surface treatment of polymer foil

Vostiňáková

Herma

Lupínková

et al. 2022

Surface & Interface Analysis

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Octenidine dihydrochloride (OCT) has a wide spectrum of antibacterial, antifungal and virucidal activity. OCT is also newly used in tissue engineering. The aim of this work was to create a new nanocomposite consisting of OCT‐grafted polymer with (i) antibacterial effect and/or (ii) surface for better cell adhesion and proliferation. The polymer foils were chemically activated with Piranha solution and subsequently grafted with OCT. Changes in surface properties before and after modifications were detected by electrokinetic analysis, goniometry, atomic force microscopy, scanning electron microscopy and X‐ray photoelectron spectroscopy. The final nanocomposite polymer/OCT exhibits antibacterial activity against Staphylococcus epidermidis (S. epidermidis). The new nanocomposite material has also been shown to support the growth of B14 cell culture on the substrate and to form cell multilayers, which could lead to the formation of spheroids. This behaviour strongly depends on the concentration of OCT grafted onto the polymer surfaces. This new nanocomposite could be used in medicine, for bioapplications, environmental protection.

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Section: Resultssupporting

confidence: 74%

Antibacterial nanocomposite supporting cell growth and spheroid formation by chemical surface treatment of polymer foil

Vostiňáková

Herma

Lupínková

et al. 2022

Surface & Interface Analysis

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“…Therefore, a variety of in vitro tests was proposed – to reduce the number of variables and to provide insight into the possibilities of fighting them off with the use of antimicrobial substances ( Stuermer et al., 2021 ; Vyas et al., 2022 ; Pham et al., 2023 ). Of note, certain in vitro wound biofilm models are performed on smooth, abiotic surfaces (which do not resemble the wound surface), and they lack also such relevant factors as flow or adequate medium resembling the wound fluid.…”

Section: Discussionmentioning

confidence: 99%

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

Paleczny

Junka²,

Krzyżek³

et al. 2023

Front. Cell. Infect. Microbiol.

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Chronic wound infection is highly associated with morbidity and endangers the patient's life. Therefore, wound care products must have a potent antimicrobial and biofilm-eradicating effect. In this work, the antimicrobial/antibiofilm activity of two low-concentrated chlorine-based and releasing solutions was investigated on a total of 78 strains of methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, using the cohesive spectrum of in vitro settings, including microtiter plate models, biofilm-oriented antiseptic test, cellulose-based biofilm model, biofilm bioreactors and Bioflux model. The antiseptic containing polyhexamethylene biguanide was used in the character of usability control of performed tests. The results obtained by static biofilm models indicate that low-concentrated chlorine-based and releasing solutions display none to moderate antibiofilm activity, while data obtained by means of the Bioflux model, providing flow conditions, indicate the moderate antibiofilm activity of substances compared with the polyhexanide antiseptic. Considering in vitro data presented in this manuscript, the earlier reported favorable clinical results of low-concentrated hypochlorites should be considered rather an effect of their rinsing activity combined with low cytotoxicity but not the antimicrobial effect per se. For the treatment of heavily biofilm-infected wounds, polyhexanide should be considered the agent of choice because of its higher efficacy against pathogenic biofilms.

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“…In wound care, microbial biofilms are recognized as one of the main causes of ineffective treatment and wound chronicity . It is proposed that at least 78% of chronic wounds contain biofilms . Mature biofilms develop already during the first 10 h and persist indefinitely when the wound remains untreated.…”

Section: Introductionmentioning

confidence: 99%

“…Clinical decisions for treatment are usually made based on planktonic bacteria in the wound, and this approach enables the treatment of acute wound infections. ,, However, to successfully treat wound infections in nonhealing wounds, the biofilm needs to be removed from the wound via surgical debridement . As the next step, local antimicrobial therapy is needed to kill the released bacteria and attack the bacteria still residing in the biofilm residuals.…”

Section: Introductionmentioning

confidence: 99%

“…As the next step, local antimicrobial therapy is needed to kill the released bacteria and attack the bacteria still residing in the biofilm residuals. Because bacterial biofilms in chronic and nonhealing wounds continue to be a medical challenge, the search for local therapies against biofilm formation and the treatment of formed biofilms are becoming increasingly important …”

Section: Introductionmentioning

confidence: 99%

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Development of In Vitro and Ex Vivo Biofilm Models for the Assessment of Antibacterial Fibrous Electrospun Wound Dressings

et al. 2023

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Increasing evidence suggests that the chronicity of wounds is associated with the presence of bacterial biofilms. Therefore, novel wound care products are being developed, which can inhibit biofilm formation and/or treat already formed biofilms. A lack of standardized assays for the analysis of such novel antibacterial drug delivery systems enhances the need for appropriate tools and models for their characterization. Herein, we demonstrate that optimized and biorelevant in vitro and ex vivo wound infection and biofilm models offer a convenient approach for the testing of novel antibacterial wound dressings for their antibacterial and antibiofilm properties, allowing one to obtain qualitative and quantitative results. The in vitro model was developed using an electrospun (ES) thermally crosslinked gelatin–glucose (GEL-Glu) matrix and an ex vivo wound infection model using pig ear skin. Wound pathogens were used for colonization and biofilm development on the GEL-Glu matrix or pig skin with superficial burn wounds. The in vitro model allowed us to obtain more reproducible results compared with the ex vivo model, whereas the ex vivo model had the advantage that several pathogens preferred to form a biofilm on pig skin compared with the GEL-Glu matrix. The in vitro model functioned poorly for Staphylococcus epidermidis biofilm formation, but it worked well for Escherichia coli and Staphylococcus aureus, which were able to use the GEL-Glu matrix as a nutrient source and not only as a surface for biofilm growth. On the other hand, all tested pathogens were equally able to produce a biofilm on the surface of pig skin. The developed biofilm models enabled us to compare different ES dressings [pristine and chloramphenicol-loaded polycaprolactone (PCL) and PCL−poly(ethylene oxide) (PEO) (PCL/PEO) dressings] and understand their biofilm inhibition and treatment properties on various pathogens. Furthermore, we show that biofilms were formed on the wound surface as well as on a wound dressing, indicating that the demonstrated methods mimic well the in vivo situation. Colony forming unit (CFU) counting and live biofilm matrix as well as bacterial DNA staining together with microscopic imaging were performed for biofilm quantification and visualization, respectively. The results showed that both wound biofilm models (in vitro and ex vivo) enabled the evaluation of the desired antibiofilm properties, thus facilitating the design and development of more effective wound care products and screening of various formulations and active substances.

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Comparative analysis of biofilm models to determine the efficacy of antimicrobials

Cited by 12 publications

References 30 publications

Antibacterial nanocomposite supporting cell growth and spheroid formation by chemical surface treatment of polymer foil

Antibacterial nanocomposite supporting cell growth and spheroid formation by chemical surface treatment of polymer foil

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic

Development of In Vitro and Ex Vivo Biofilm Models for the Assessment of Antibacterial Fibrous Electrospun Wound Dressings

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