Comparative analysis of clinics, pathologies and immune responses in BALB/c and C57BL/6 mice infected with Streptobacillus moniliformis

Fornefett, Juliane; Krause, Jaqueline; Klose, Kristin; Fingas, Felix; Hassert, Rayk; Eisenberg, Tobias; Schrödl, Wieland; Grünwald, Thomas; Müller, Ulrich; Baums, Christoph Georg

doi:10.1016/j.micinf.2017.10.001

Cited by 20 publications

(23 citation statements)

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“…The number of animals included in this study was based on the objectives to reveal putative differences between BALB/c and C57BL/6 mice in susceptibility, to evaluate sentinel monitoring and to obtain convalescence sera for a further immunoproteomics study. All animals were housed as described in a previous study in detail [ 32 ], which included ventilated cages with HEPA filters and air conditioning as well as ad libitum feeding and drinking. One week before infection blood samples were collected from the submandibular vein and stored at − 20 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Whole cell extract was prepared from pelleted bacteria as described for Streptobacillus moniliformis previously [ 32 ]. Three hundred nanogramms of P. pneumotropica antigen (either whole cell extract or a concentrated culture supernatant) or casein (background measurement) were used per well to coat Corning Costar® assay plates in carbonate buffer (pH 8.1).…”

Section: Methodsmentioning

confidence: 99%

“…Three hundred nanogramms of P. pneumotropica antigen (either whole cell extract or a concentrated culture supernatant) or casein (background measurement) were used per well to coat Corning Costar® assay plates in carbonate buffer (pH 8.1). Blocking with casein and washing of ELISA plates was conducted as described [ 32 ]. Twofold serial dilutions of sera and TNLs in PBS with 2 mM EDTA, 0.1% Tween20 and 0.1% bovine casein were applied to the ELISA plates including also reference sera/reference TNLs as well as negative controls.…”

Section: Methodsmentioning

confidence: 99%

“…For detection of P. pneumotropica -specific serum-IgG, serum-IgG2c or TNL-IgA, plates were incubated for 1 h at RT with a 1:10,000 dilution of a HRP-conjugated goat anti-mouse IgG antibody (Jackson Immuno Research Laboratories), or a 1:10,000 dilution of a HRP-conjugated goat anti-mouse IgG2c antibody (Biorad; C57BL/6 mice only) or a 1:5000 dilution of HRP-conjugated goat anti-mouse IgA antibody (BIOMOL GmbH), respectively. ELISA plates were developed with 3,3′,5,5′-tetramethylbenzidine as described [ 32 ]. Absorbance was measured at 450 nm (reference at 630 nm).…”

Section: Methodsmentioning

confidence: 99%

“…Determination of IgG subtypes and calculation of respective ratios was conducted as described previously [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

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Comparative analysis of humoral immune responses and pathologies of BALB/c and C57BL/6 wildtype mice experimentally infected with a highly virulent Rodentibacter pneumotropicus (Pasteurella pneumotropica) strain

et al. 2018

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BackgroundMice are a natural host for Rodentibacter (R.) pneumotropicus. Despite specific monitoring, it is still one of the most important infectious agents in laboratory animals. The objective of this study was to determine the virulence of a prevalent pathotype of R. pneumotropicus and characterize the host response in a new animal model.ResultsIntranasal infection of C57BL/6 and BALB/c mice with a R. pneumotropicus strain (JF4Ni) bearing the genes of the three known repeats in toxin (RTX) toxins resulted in an unprecedented high mortality and morbidity above 50 and 80%, respectively. Morbidity was associated with severe weight loss as well as conjunctivitis and dyspnea. A main pathology was a catarrhal purulent to necrotic bronchopneumonia. Specific immune globuline (Ig) A was detected in tracheonasal lavages of most surviving mice which were still colonized by R. pneumotropicus. Furthermore, all surviving animals showed a distinct production of IgG antibodies. To differentiate T-helper cell (Th) 1 and Th2 immune responses we used subclasses of IgGs as indicators. Mean ratios of IgG2b to IgG1 were below 0.8 in sera drawn from both mice strains prior infection and from BALB/c mice post infection. In contrast, C57BL/6 mice had a mean IgG2b/IgG1 ratio of 1.6 post infection indicating a Th1 immune response in C57BL/6 versus a Th2 response in BALB/c mice associated with a tenfold higher bacterial load in the lung. In accordance with a Th1 response high antigen-specific IgG2c titers were detected in the majority of surviving C57BL/6 mice.ConclusionsR. pneumotropicus JF4Ni is a highly virulent strain causing severe pneumonia and septicemia after intranasal infection of C57BL/6 and BALB/c mice. Persisting infections in the two mice strains are associated with Th1 and Th2 immune responses, respectively, and differences in the bacterial burden of the lung. The described model is ideally suited for future vaccination studies using the natural host.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1186-8) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Determination of IgG subtypes and calculation of respective ratios was conducted as described previously [ 32 ].…”

Section: Methodsmentioning

confidence: 99%