Comparative Analysis of Enterococcus faecalis Biofilm Formation on Different Substrates

Guerreiro-Tanomaru, Juliane Maria; Faria-Júnior, Norberto Batista de; Duarte, Marco Antônio Húngaro; Ordinola‐Zapata, Ronald; Graeff, Márcia Sirlene Zardin; Tanomaru-Filho, Mário

doi:10.1016/j.joen.2012.09.027

Cited by 70 publications

(76 citation statements)

References 33 publications

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“…36 Accordingly, mature biofilms confer greater resistance against antimicrobial agents to bacterial cells, which are organized into overlapping layers deeply embedded in the matrix. 24,37 Thus, the use of mature biofilm (14 days old) 18 and dentin as the substrate may explain the difficulties for its complete biofilm elimination observed in the present study.…”

Section: Stojicic Et Al 15 Observed Greater Antibiofilm Activity Formentioning

confidence: 73%

“…Hydroxyapatite, 16,18 human dentin, 21,22 bovine dentin 6,14 and polystyrene membrane 21 have been used as substrates for biofilm formation. E. faecalis biofilm shows organization after growing on bovine dentin blocks for 14 days.…”

Section: Discussionmentioning

confidence: 99%

“…E. faecalis biofilm shows organization after growing on bovine dentin blocks for 14 days. 18 Bovine dentin has been widely used 14,17,18 due to its similarities with human dentin. Biofilm maturity may influence its response to antimicrobial agents.…”

Section: Discussionmentioning

confidence: 99%

“…18 The culture medium (BHI) of each specimen was replaced every 48 hours. The purity of the E. faecalis strain was tested by Gram stain and colony morphology.…”

Section: 18mentioning

confidence: 99%

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Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Nascimento

Tanomaru‐Filho

Faria-Júnior

et al. 2014

The Journal of Contemporary Dental Practice

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Aim: To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212]. Materials and methods:The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey's tests at 5% significance.Results: All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05), but was unable to completely eliminate the microorganisms. CTR and QMiX did not differ from each other.Conclusion: CTR addition to CHX and NaOCl solutions did not improve the antimicrobial activity against biofilm. All evaluated irrigants and associations presented activity against planktonic E. faecalis. Only NaOCl and NaOCl + CTR eliminated biofilm after 1 and 3 minutes of direct contact.Clinical relevance: Addition of CTR does not modify the antibiofilm effectiveness of CHX and NaOCl.

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Section: Stojicic Et Al 15 Observed Greater Antibiofilm Activity Formentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…18 The culture medium (BHI) of each specimen was replaced every 48 hours. The purity of the E. faecalis strain was tested by Gram stain and colony morphology.…”

Section: 18mentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Nascimento

Tanomaru‐Filho

Faria-Júnior

et al. 2014

The Journal of Contemporary Dental Practice

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“…The cell culture plates with the submerged bovine dentine blocks were kept in 5% CO 2 (Ultra Safe, HF212-UV) at 37°C for 14 days. 19,20 The BHI medium of each specimen was completely changed every 48 h without adding new microorganisms.…”

Section: Analysis Of Antibiofilm Activitymentioning

confidence: 99%

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm Abstract: Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO 2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14 th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

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A new model for the formation of an Enterococcus faecalis endodontic biofilm with nutritional restriction

et al. 2021

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An in vitro model for the formation of an Enterococcus faecalis endodontic biofilm under nutritional restriction was established, simulating clinical conditions for the evaluation of antimicrobial substances. Biofilm formation in dentin was standardized using root quarters incubated with E. faecalis ATCC 29212 at 37°C without nutritional changes. Biofilms were evaluated at 7, 14, and 30 days, counting bacterial colony-forming units using conventional culture and verified scanning electron microscopy. Bacterial viability and biovolume were determined with confocal laser microscopy. Colonization of E. faecalis and biofilm formation on the dentinal surface was confirmed after 7 and 14 days, respectively. Microorganism colonization was homogeneous over the entire root surface at each time point, without significant differences in the viability percentage and biovolume. On the contrary, a decrease in viability and an increase in biovolume were observed when the time was increased. Compared with other incubation times, 14 days was found to be the best time for the establishment of the biofilm in terms of biovolume and bacterial viability. This in vitro model for the formation of endodontic biofilm will allow future evaluation of the efficacy of antimicrobial substances with a more adequate clinical approach.

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Comparative Analysis of Enterococcus faecalis Biofilm Formation on Different Substrates

Cited by 70 publications

References 33 publications

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

A new model for the formation of an Enterococcus faecalis endodontic biofilm with nutritional restriction

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