Comparative analysis of extractable proteins from Clostridium perfringens type A and type C strains showing varying degree of virulence

Objectives To identify immunogenic proteins of C. botulinum type B secretome by immunoproteomic analysis. Results In the present study, an attempt was made to elucidate the vaccine candidates/diagnostic molecules against botulism using immuno proteomic approach. C. botulinum type B secretome was elucidated when it was grown in TPGY as well as CMM media. Predominant 51 proteins were identified in both the media using 2-DE and mass spectrometry analysis. 2D gels (CMM & TPGY) were probed with respected proteins mice antiserum and obtained 17 and 10 immunogenic proteins in TPGY as well as CMM media respectively. Hypothetical protein CLOSPO_00563, ornithine carbamoyl transferase, FlaA, molecular chaperone GroEL and secreted protease proteins were found as the common immuno dominant proteins in both media. Polyclonal Antibodies raised against C. botulinum types A and E showed cross-reactivity with secretome C. botulinum type B at the lowest dilution (1:1000) but did not show cross reactivity with highest dilution (1:30,000) with C. botulinum type B secretome. Polyclonal antibodies against C. botulinum type F secretome did not show cross reactivity with C. botulinum type B secretome. Conclusions Identified immunogenic proteins can be used as vaccine candidates and diagnostic markers for the infant and wound botulism but common immunogenic proteins may be the best vaccine candidate molecule for development of vaccine as well as diagnostic system against the infant and wound botulism. Supplementary Information The online version contains supplementary material available at 10.1007/s10529-021-03091-4.

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Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism

Sharma

Ponmariappan

Rani

et al. 2021

Biotechnol Lett

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Secretome, surfome and immunome: emerging approaches for the discovery of new vaccine candidates against bacterial infections

Dwivedi¹,

Alam

Tomar³

2016

World J Microbiol Biotechnol

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Functional genomics has made possible advanced structure-to-function investigation of pathogens and helped characterize virulence mechanisms. Proteomics has been become a tool for large-scale identification of proteins involved during invasion and infection by the pathogens. Bacterial surface and secreted proteins play key role in the interaction between the bacterial cell and the host environment. Thus exoproteome and surface proteome of a microorganism are hypothesized to contain components of effective vaccines. Surfome and exoproteome analysis strategy facilitates identification of novel vaccine antigen and overall helps in progress of discovery of vaccine. The study of the antibody response can advance how proteomics is used, because it investigates antibody-antigen interactions and also unravel the relationship of antibody responses to pathogen and host characteristics. System immunology integrating with proteome i.e. immunoproteomics is applicable to those infections that are having tendency of diverse antibody target recognition and thus accurately reflects progression of the infection.

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Comparative analysis of extractable proteins from Clostridium perfringens type A and type C strains showing varying degree of virulence

Cited by 2 publications

References 52 publications

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism

Immunoproteomic analysis of Clostridium botulinum type B secretome for identification of immunogenic proteins against botulism

Secretome, surfome and immunome: emerging approaches for the discovery of new vaccine candidates against bacterial infections

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